Analysis of Protein Expression in Serum of KOA Subjects

NCT ID: NCT06553313

Last Updated: 2024-08-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

50 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-08-04

Study Completion Date

2025-07-23

Brief Summary

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In the previous study "Using omics technology to explore the mechanism of acupuncture treatment of different acupoints of knee osteoarthritis: a randomized controlled trial (CMUH109-REC3-113)", we found that acupuncture for knee osteoarthritis(KOA) significantly reduces inflammation, apoptosis, and metabolism-related serum protein profiles. However, due to the lack of serum protein data from the control group, it is impossible to establish standard values for comparison. To analyze the protein expression in the serum of KOA control group subjects, we also measured the spectral energy values of the radial artery pulse wave obtained from the pulse sphygmograph and the meridian energy values obtained from Ryodoraku. The results obtained can be compared with previous studies, thus this experiment was designed.

Detailed Description

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Conditions

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Knee Osteoarthritis

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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the control group of knee arthritis

Monitoring the stability of protein expression, pulse wave and meridian energy in the serum of the KOA control group (participants without knee pain).

Group Type OTHER

Micro-Western Array

Intervention Type DEVICE

The subjects first took the energy measurement of the pulse sphygmograph and Ryodoraku, rested for 20 minutes, and then repeated the measurements again. In order to comply with the acupuncture treatment course was 5 weeks in the previous study, we have two blood draw point. Each subject's blood was drawn for the first time, and then for the second time in 5 weeks later. Each subject drew 10ml of blood each time. The blood samples are analyzed by Micro-Western Array (MWA) and Enzyme-linked immunosorbent assay (ELISA) to obtain the serum protein content value of healthy individuals.

Interventions

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Micro-Western Array

The subjects first took the energy measurement of the pulse sphygmograph and Ryodoraku, rested for 20 minutes, and then repeated the measurements again. In order to comply with the acupuncture treatment course was 5 weeks in the previous study, we have two blood draw point. Each subject's blood was drawn for the first time, and then for the second time in 5 weeks later. Each subject drew 10ml of blood each time. The blood samples are analyzed by Micro-Western Array (MWA) and Enzyme-linked immunosorbent assay (ELISA) to obtain the serum protein content value of healthy individuals.

Intervention Type DEVICE

Other Intervention Names

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Enzyme-linked immunosorbent assay pulse sphygmograph Ryodoraku

Eligibility Criteria

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Inclusion Criteria

* Adults over 50 years old without knee pain

Exclusion Criteria

1. Clinical diagnosis of knee osteoarthritis
2. Taking anti-inflammatory and analgesic drugs in the past month
3. Taking blood-lipid-lowering drugs or health foods in the past month
4. Received any acupuncture treatment in the past 3 weeks
Minimum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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China Medical University Hospital

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Wan-Zhen Yu

Role: PRINCIPAL_INVESTIGATOR

China Medical University, China

Locations

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China Medical University

Taichung, , Taiwan

Site Status RECRUITING

Countries

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Taiwan

Central Contacts

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Wan-Zhen Yu

Role: CONTACT

8864-22053366 ext. 3500

Yu-Chen Lee

Role: CONTACT

8864-22053366 ext. 3500

Facility Contacts

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Yu-Chen Lee

Role: primary

8864-22053366 ext. 3500

Other Identifiers

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CMUH113-REC3-115

Identifier Type: -

Identifier Source: org_study_id

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