Senescent Endometrial Cells in Patients with Thin Endometrial Lining

NCT ID: NCT06450301

Last Updated: 2024-11-21

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

100 participants

Study Classification

OBSERVATIONAL

Study Start Date

2024-09-11

Study Completion Date

2027-06-30

Brief Summary

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The goal of this observational study is to determine if thin endometrial lining that is unresponsive to estrogen might be associated with the presence of senescent cells in patients following long-term use of oral contraceptives. The main question it aims to answer is:

Are there any senescent cells present in thin endometrial lining?

During the luteal phase of the cycle, participants will will undergo:

* Gynecologic US to measure the endometrial lining thickness and pattern.
* Endometrial biopsy with Pipelle catheter

Detailed Description

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The investigators hypothesized that senescent cells in the endometrium may be the cause of the failure of thin endometrium to respond to normal growth stimuli such as estrogen.

The outcome that research team anticipate to measure is the percentage of senescent cells in the endometrial biopsies of women with thin endometrium compared to the control group biopsies.

Conditions

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Asherman's Syndrome Thin Endometrial Lining Cell Senescence

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Thin endometrial lining

Patients following long-term use of oral contraceptives with known thin endometrial lining (less than 7mm, confirmed in at least two cycles and unresponsive to estrogen-induced growth).

We will ask each woman to undergo an endometrial biopsy as part of the study during their luteal phase.

Endometrial Biopsy

Intervention Type PROCEDURE

An endometrial biopsy is the removal of a small piece of tissue from the endometrium, which is the lining of the uterus. Endometrial biopsies are commonly done to check for the window of implantation, to determine ovulation or for enhancing implantation (procedure called endometrial scratching). For this purpose, we will use a "Pipelle" catheter. This procedure is very common in gynecologic practice and

generally considered safe. In most of the cases this procedure is indicated for evaluation of irregular vaginal bleeding, vaginal bleeding in post-menopausal women and for infertility related problems (recurrent implantation failures for example).

Asherman's syndrome

Patients with Asherman's syndrome following Dilation and curettage (D\&C). We will ask each woman to undergo an endometrial biopsy as part of the study during their luteal phase.

Endometrial Biopsy

Intervention Type PROCEDURE

An endometrial biopsy is the removal of a small piece of tissue from the endometrium, which is the lining of the uterus. Endometrial biopsies are commonly done to check for the window of implantation, to determine ovulation or for enhancing implantation (procedure called endometrial scratching). For this purpose, we will use a "Pipelle" catheter. This procedure is very common in gynecologic practice and

generally considered safe. In most of the cases this procedure is indicated for evaluation of irregular vaginal bleeding, vaginal bleeding in post-menopausal women and for infertility related problems (recurrent implantation failures for example).

Control

Patients already scheduled to undergo an endometrial biopsy for infertility management.

We will ask each woman to undergo an endometrial biopsy as part of the study during their luteal phase. In some cases, we may be able to access archived endometrial samples from women in the control group who agree to participate and who have already had an endometrial biopsy.

Endometrial Biopsy

Intervention Type PROCEDURE

An endometrial biopsy is the removal of a small piece of tissue from the endometrium, which is the lining of the uterus. Endometrial biopsies are commonly done to check for the window of implantation, to determine ovulation or for enhancing implantation (procedure called endometrial scratching). For this purpose, we will use a "Pipelle" catheter. This procedure is very common in gynecologic practice and

generally considered safe. In most of the cases this procedure is indicated for evaluation of irregular vaginal bleeding, vaginal bleeding in post-menopausal women and for infertility related problems (recurrent implantation failures for example).

Interventions

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Endometrial Biopsy

An endometrial biopsy is the removal of a small piece of tissue from the endometrium, which is the lining of the uterus. Endometrial biopsies are commonly done to check for the window of implantation, to determine ovulation or for enhancing implantation (procedure called endometrial scratching). For this purpose, we will use a "Pipelle" catheter. This procedure is very common in gynecologic practice and

generally considered safe. In most of the cases this procedure is indicated for evaluation of irregular vaginal bleeding, vaginal bleeding in post-menopausal women and for infertility related problems (recurrent implantation failures for example).

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Age 18-39
* BMI - up to 35
* Thin endometrium after OCP use
* Asherman's syndrome
* Women already undergoing endometrial biopsy for infertility testing (eg. EMMA testing)
* Women with archived endometrial biopsy samples

Exclusion Criteria

* Women with systemic disease (Rheumatic disease, DM)
* Women with coagulopathies
* Women with active pelvic inflammatory process
* Uterine anomalies
* Pregnant women
* Any hormonal contraception use or IUD
Minimum Eligible Age

18 Years

Maximum Eligible Age

39 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Trio Fertility

OTHER

Sponsor Role lead

Responsible Party

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Dr. Robert F. Casper

Scientific Director, MD, FRCSC, REI

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Trio Fertility

Toronto, Ontario, Canada

Site Status RECRUITING

Countries

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Canada

Facility Contacts

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Robert. F. Casper, Dr.

Role: primary

416-506-0804 ext. 3228

Yasaman Sadeghi, M.Sc.

Role: backup

416-506-0804 ext. 2268

Robert. F. Casper, Dr.

Role: backup

Other Identifiers

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3533

Identifier Type: -

Identifier Source: org_study_id

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