Phase 1 Trial to Assess the Safety and Immunogenicity of an Inactivated, Adjuvanted Whole Zika Virus Vaccine Candidate (VLA1601) in Healthy Adults

NCT ID: NCT06334393

Last Updated: 2025-08-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ACTIVE_NOT_RECRUITING

Clinical Phase

PHASE1

Total Enrollment

150 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-03-25

Study Completion Date

2026-04-27

Brief Summary

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This phase 1 clinical trial consists of an initial open-label sentinel run-in (n=25) and a randomized, double-blind, dose-finding (n=125) investigating three antigen dose levels (low, medium and high) of VLA1601 and bedside mixing of the low-dose formulation with one of the two additional adjuvants (CpG1018®, 3M-052-AF/AP 60-702). VLA1601 will be administered according to a two-dose regimen (i.e., on Day 1 and Day 29).

The primary objective of this trial is to assess the safety and tolerability of the vaccine candidate up to 7 days after each vaccination; and to assess the immune response induced by the vaccine candidate 28 days after the second vaccination. Additionally, safety and immune response of the vaccine candidate will be monitored throughout the trial.

Detailed Description

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VLA1601 is a second generation, highly purified, inactivated, whole ZIKV vaccine candidate (adsorbed on aluminum hydroxide) designed for active immunization for the prevention of disease caused by the flavivirus ZIKV.

This is a phase 1 trial, consisting of an initial open-label sentinel run-in (n=25) phase and a randomized, double-blind, dose-finding trial (n=125) in flavivirus naïve adults aged 18 to 49 years. In total approximately 150 participants will be vaccinated in this trial.

The trial will investigate three antigen dose levels (low, medium and high) of VLA1601. In addition, CpG 1018® or 3M-052-AF/AP 60-702 are investigated as add-on adjuvants in the low dose group (bedside mixing). Each dose is formulated with alum (aluminum hydroxide) adjuvant.

In each of the five treatment arms 30 participants (each with 5 sentinel/run-in and 25 randomized participants) will be vaccinated. Each participant will receive 2 vaccinations, one on Day 1 and one on Day 29, which will be administered intramuscularly (i.m.) in the deltoid muscle (non-dominant arm). The screening period can last up to 21 days.

The trial began with the vaccination of 25 sentinel participants (5 participants in each of the 5 treatment arms) in a sequential open-label, staggered dose-escalation manner.

Up to approximately 125 participants will be randomized 1:1:1:1:1, stratified by trial site to 5 treatment arms. The injection volume in each treatment arm will be 0.45 mL at each of the 2 vaccinations.

The primary objective of this trial is to assess the safety and tolerability of the vaccine candidate up to 7 days after each vaccination; and to assess the immune response induced by the vaccine candidate 28 days after the second vaccination.

Following a sponsor review of available safety and immunogenicity data up to 6 months after the second vaccination, all sentinels and randomized participants from most favorable treatment arm(s) will be selected for an on-site visit at Day 395 for long-term safety and immunogenicity assessment. All other treatment arms will be followed only by phone-call for the Day 395 assessment of long-term safety.

Conditions

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Zika Zika Virus Infection

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

TRIPLE

Participants Investigators Outcome Assessors

Study Groups

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VLA1601 Low dose

Group Type EXPERIMENTAL

VLA1601

Intervention Type BIOLOGICAL

0.45mL (milliliter), Day 1 and 29

VLA1601 Low dose + CpG 1018®

Group Type EXPERIMENTAL

VLA1601

Intervention Type BIOLOGICAL

0.45mL (milliliter), Day 1 and 29

CpG 1018®

Intervention Type BIOLOGICAL

CpG 1018® will be investigated in combination with VLA1601 Low dose

VLA1601 Low dose + 3M-052-AF

Group Type EXPERIMENTAL

VLA1601

Intervention Type BIOLOGICAL

0.45mL (milliliter), Day 1 and 29

3M-052-AF

Intervention Type BIOLOGICAL

3M-052-AF will be investigated in combination with VLA1601 Low dose

VLA1601 Medium dose

Group Type EXPERIMENTAL

VLA1601

Intervention Type BIOLOGICAL

0.45mL (milliliter), Day 1 and 29

VLA1601 High dose

Group Type EXPERIMENTAL

VLA1601

Intervention Type BIOLOGICAL

0.45mL (milliliter), Day 1 and 29

Interventions

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VLA1601

0.45mL (milliliter), Day 1 and 29

Intervention Type BIOLOGICAL

CpG 1018®

CpG 1018® will be investigated in combination with VLA1601 Low dose

Intervention Type BIOLOGICAL

3M-052-AF

3M-052-AF will be investigated in combination with VLA1601 Low dose

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* 18 to 49 years of age
* BMI of ≥18.5 and \<30 kg/m2
* generally healthy as determined by the investigator's clinical judgement based on medical history, physical examination, and screening laboratory tests.
* If trial participant is of childbearing potential: negative pregnancy test; employ adequate birth control measures up to Day 208.
* Male participant agrees to employ adequate birth control measures up to 90 days after last vaccination.

Exclusion Criteria

Participant

* has a known history of the following flavivirus infection: Zika Virus (ZIKV), Japanese Encephalitis Virus (JEV), Dengue Virus (DENV), Yellow Fever Virus (YFV), West-Nile Virus (WNV), or Tick-Borne Encephalitis Virus (TBEV).
* received or has plans to receive a licensed or investigational flavivirus vaccine during the course of the trial.
* travelled within 4 weeks prior to trial enrollment or has plans to travel to areas (including within the US) with Zika virus (ZIKV), Japanese Encephalitis Virus (JEV), Dengue Virus (DENV) or Yellow Fever Virus (YFV) active transmission/circulation during the course of the trial .
* received active or passive immunization within 4 weeks prior or planned to get such vaccination after any trial-vaccination.
* presents with clinically significant abnormal laboratory values, as determined by the investigator.
* tests positive for human immunodeficiency virus (HIV), hepatitis B surface antigen (HBsAg) or hepatitis C virus (HCV).
* has history of significant cardiovascular, respiratory (including asthma), metabolic, neurological (including Guillain-Barre syndrome \[GBS\]), psychiatric, hepatic, rheumatic, autoimmune, hematological, gastrointestinal, or renal disorder.
* with known or suspected defect of the immune system that would prevent an immune response to the vaccine.
* received immuno-suppressive therapy within 4 weeks prior to first vaccination. Radiation therapy or immunosuppressive cytotoxic drugs/ monoclonal antibodies in the previous 3 years.
* with a history of severe hypersensitivity reactions or anaphylaxis.
* with a history of any vaccine related contraindicating event .
* with acute febrile infections within two weeks prior to vaccination in this trial.
* donated blood within 4 weeks or received blood-derived products (e.g. plasma) within 12 weeks prior to vaccination in this trial or plans to donate blood or use blood products during the course of the trial.
* has a rash, dermatological condition or tattoos that would, in the opinion of the investigator, interfere with injection site reaction rating.
* presents with clinical conditions representing a contraindication to intramuscular vaccination and blood draws.
* is currently enrolled (ICF signed) or has participated in another clinical trial involving an investigational medicinal product (IMP) or device within 4 weeks prior to trial enrollment or is scheduled to participate in another clinical trial involving an IMP or investigational device during the course of this trial.
* has a known or suspected problem with alcohol or drug abuse
Minimum Eligible Age

18 Years

Maximum Eligible Age

49 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Valneva Austria GmbH

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Flourish Research

Chicago, Illinois, United States

Site Status

Velocity Clinical Research

Sioux City, Iowa, United States

Site Status

Velocity Clinical Research

Lincoln, Nebraska, United States

Site Status

Velocity Clinical Research

Omaha, Nebraska, United States

Site Status

Countries

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United States

Other Identifiers

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VLA1601-102

Identifier Type: -

Identifier Source: org_study_id

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