Prevalence of FGIDs and Probiotics Study

NCT ID: NCT06309199

Last Updated: 2025-07-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

512 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-03-14

Study Completion Date

2026-02-28

Brief Summary

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This is a study to evaluate the prevalence of FGIDs in infants using the Thai version of Rome IV diagnostic questionnaire for functional gastrointestinal disorders in infants and evaluate the efficacy of Limosilactobacillus reuteri DSM 17938 to prevent FGIDs in infants.

Detailed Description

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This is a randomized controlled trial study about the the efficacy of Lactobacillus reuteri DSM 1987 to prevent FGIDs in healthy infants and the effect to gut microbiota diversity. The incidence of FGIDs in Thai infant using Thai version of ROME IV questionnaire (authorized by Rome Foundation) for infant and toddler and the associated factors of FGIDs also evaluate.

Population: target and control populations are the healthy infants born at King Chulalongkorn Memorial Hospital, Bangkok, Thailand, and can come to follow up at the hospital at 1, 2, 4, and 12 months of age Inclusion criteria

1. Healthy and term (GA 37-41 weeks) infants
2. Appropriate weight for age
3. APGAR score more than 8 at 10 minutes of life
4. Normal physical examination
5. Mothers have no previous probiotics use Methodology

* Participants were double-blinded randomized using computer generation in block of four
* Target population were received Limosilactobacillus reuteri DSM 17938 5 drops (0.185 mL) per day for 60 days
* Control population were received mixture of sunflower oil and MCT oil 5 drop (0.185 mL) per day for 60 days
* There were 5 visits at birth, 1, 2, 4, 12 months of life: the demographic data/Rome IV questionnaire for FGIDs in infant and associated factors with FGIDs record, physical examination and stool collection The study will be analyzed (open the randomized group) after all participants complete the 4-month follow-up period
* Research assistance will telephone to them to take the Rome IV questionnaire for FGIDs at 3, 6, 9 months of life

Biological specimen collection Stool will be collected by rectal stimulation in each visit. The amount of \>5 gm stool will be preserved in DNA and kept at -80 °C. All stool specimen will be processed the DNA extraction (DNA \> 40 microlitre with concentration \>50 ng/L). The DNA extraction for further amplification 16S rRNA using Illumina. The rest of the specimen will be kept up to 10 years for further evaluation.

Conditions

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Functional Gastrointestinal Disorders Infant Conditions

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Intervention group received probiotics Control group received sunflower and MCT oil
Primary Study Purpose

TREATMENT

Blinding Strategy

TRIPLE

Participants Caregivers Investigators
These 2 groups was assigned using computer generate with block of 4 and ratio 1:1.

The intervention and placebo were prepare in the same container with the same color, odor and blinded to investigators, participants and guardians.

Study Groups

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Probiotics

Limosilactobacillus reuteri DSM 17938 with sunflower oil and MCT oil were packed into a container. Infants will received 5 drops per day in the morning for 60 +/- 20 days

Group Type EXPERIMENTAL

Biogaia

Intervention Type DIETARY_SUPPLEMENT

Biogaia is a probiotics product that contain Limosilactobacillus reuteri DSM 17938, sunflower oil and MCT oil

Placebo

Sunflower oit and MCT oil were paced into a container. Infants will received 5 drops per day in the morning for 60+/- 20 days

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type OTHER

Limosilactobacillus reuteri DSM 17938, sunflower oil and MCT oil

Interventions

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Biogaia

Biogaia is a probiotics product that contain Limosilactobacillus reuteri DSM 17938, sunflower oil and MCT oil

Intervention Type DIETARY_SUPPLEMENT

Placebo

Limosilactobacillus reuteri DSM 17938, sunflower oil and MCT oil

Intervention Type OTHER

Other Intervention Names

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Sunflower oil + MCT oil

Eligibility Criteria

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Inclusion Criteria

1. Healthy and term (GA 37-41 weeks) infants
2. Appropriate weight for age
3. APGAR score more than 8 at 10 minutes of life
4. Normal physical examination
5. Mothers have no previous probiotics use

Exclusion Criteria

1. not willing to anticipate in the study
2. cannot come to follow-up until 1 year of age
Minimum Eligible Age

3 Days

Maximum Eligible Age

1 Year

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Chulalongkorn University

OTHER

Sponsor Role lead

Responsible Party

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Palittiya Sintusek, MD

Director, Head of Center of Excellence in Thai Pediatric Gastroenterology, Hepatology and Immunology

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Yong Poovorawan, MD

Role: STUDY_DIRECTOR

Center of Excellence in Clinical Virology

Locations

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Maha Chakri Sirindhorn Clinical Research Center (Chula CRC)

Bangkok, Bangkok, Thailand

Site Status RECRUITING

Countries

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Thailand

Central Contacts

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Palittiya Sintusek, PhD

Role: CONTACT

+66818288082

Sittichoke Prachuapthunyachart, MD

Role: CONTACT

+66829740649

Facility Contacts

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Role: primary

02 251 6704

Other Identifiers

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0855/66

Identifier Type: -

Identifier Source: org_study_id

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