First-line EXL01 With Nivolumab and FOLFOX for PD-L1 CPS ≥5 Metastatic Gastric Cancer

NCT ID: NCT06253611

Last Updated: 2025-09-22

Basic Information

• Recruitment Status: RECRUITING
• Clinical Phase: PHASE2
• Total Enrollment: 120 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2024-04-16
• Study Completion Date: 2029-04-30

Brief Summary

This is a randomized non-comparative, multicenter phase II study in patients with PD-L1 PD-L1 combined positive score (CPS) ≥5 advanced gastric cancer to evaluate the efficacy and safety of nivolumab and FOLFOX in combination with EXL01 as first-line treatment.

After signing the informed consent form, and upon confirmation of the patient's eligibility, patients will be randomized in a 2:1 ratio to either the nivolumab and FOLFOX plus EXL01 arm (experimental) or the nivolumab and FOLFOX arm (control). In both arms, treatment will be given until PD, unacceptable toxicity or for a maximum of 24 months (52 cycles).

Detailed Description

The primary objective of the study is to assess the objective response rate (ORR) at 4 months (based on Response Evaluation Criteria in Solid Tumor [RECIST] criteria v1.1) of patients with PD-L1 CPS ≥ 5 advanced gastric cancer treated by EXL01 plus nivolumab and FOLFOX as first-line treatment.

With a randomization ratio of 2:1 it will be necessary to randomize 40 patients in the control arm, 80 patients in the experimental arm and so a total of 120 evaluable patients in the study. Randomization will be stratified by PD-L1 expression level, center, and prior gastrectomy.

In the experimental arm, the primary analysis will be on modified intent-to-treat (mITT) population. Confirmative analysis will be conducted firstly in the ITT population and secondly, in the Per Protocol (PP). Analyses of safety will be conducted in safety population.

The Kaplan-Meier method will be used to estimate time to event endpoints and described using the median and event-free rates over time with CI.

Conditions

Gastric Cancer

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Nivolumab Combined With FOLFOX and EXL01

Nivolumab 240 mg IV q2w and FOLFOX q2w plus EXL01 orally once daily

Group Type: EXPERIMENTAL

Nivolumab

Intervention Type: DRUG

Nivolumab 240 mg IV; every 2 weeks

FOLFOX regimen

Intervention Type: DRUG

Oxaliplatin 85 mg/m², leucovorin 400 mg/m², bolus of 5-FU 400 mg/m², continuous 5-FU 2400/m² in 46 hours; every 2 weeks

EXL01

Intervention Type: BIOLOGICAL

Orally 1 capsule/day, starting on day 1 of each FOLFOX/nivolumab treatment.

Nivolumab and FOLFOX

Nivolumab 240 mg IV q2w and FOLFOX q2w

Group Type: ACTIVE_COMPARATOR

Nivolumab

Intervention Type: DRUG

Nivolumab 240 mg IV; every 2 weeks

FOLFOX regimen

Intervention Type: DRUG

Oxaliplatin 85 mg/m², leucovorin 400 mg/m², bolus of 5-FU 400 mg/m², continuous 5-FU 2400/m² in 46 hours; every 2 weeks

Interventions

Nivolumab

Nivolumab 240 mg IV; every 2 weeks

Intervention Type: DRUG

FOLFOX regimen

Oxaliplatin 85 mg/m², leucovorin 400 mg/m², bolus of 5-FU 400 mg/m², continuous 5-FU 2400/m² in 46 hours; every 2 weeks

Intervention Type: DRUG

EXL01

Orally 1 capsule/day, starting on day 1 of each FOLFOX/nivolumab treatment.

Intervention Type: BIOLOGICAL

Other Intervention Names

Opdivo; Leucovorin, fluorouracil, and oxaliplatin

Eligibility Criteria

Inclusion Criteria

1. Patients must have dated and signed an approved written informed consent form. This must be obtained before the performance of any protocol-related procedures that are not part of normal patient care.

2. Patients must be willing and able to comply with scheduled visits, treatment schedule, laboratory tests and other requirements of the study,

Target Population

3. Inoperable, advanced, or metastatic gastric cancer or gastroesophageal junction or distal esophageal carcinoma and histologically confirmed predominant adenocarcinoma,

4. Expression of PD-L1 with a combined positive score (PD-L1 CPS) ≥5, Note: information must be available at the time of inclusion, the examination will be performed locally in the center and secondarily confirmed centrally,

5. No prior systemic cancer treatment given as primary therapy for advanced nonresectable or metastatic disease, Note: if patient received neoadjuvant/adjuvant therapy, this therapy should be completed at least 6 months prior to the diagnosis of metastatic or recurrent disease is made. Palliative radiotherapy is allowed and must be completed 2 weeks prior to randomization,

6. At least one measurable lesion as assessed by computed tomography (CT)-scan or magnetic resonance imaging (MRI) according to Response Evaluation Criteria in Solid Tumors (RECIST) v 1.1 and feasibility of repeated radiological assessments; radiographic tumor assessment should be performed within 28 days prior to randomization,

7. Eastern Cooperative Oncology Group performance status (ECOG PS) of 0 or 1,

8. Adequate hematologic and end-organ function, defined by the following laboratory test results, obtained within 14 days prior to randomization of study treatment:

1. White blood cell ≥ 2000/μL;

2. Neutrophils ≥ 2000/μL;

3. Platelets ≥ 100.000/μL;

4. Hemoglobin ≥ 9.0 g/dL;

5. Serum albumin ≥ 30 g/L;

6. Serum creatinine level ≤ 150 μM and calculated creatinine clearance (Cockcroft-Gault) > 50 mL/minute,

7. Total bilirubin ≤ 1.5 x upper normal limit (ULN);

8. Alanine aminotransferase (ALT) ≤ 3.0 x ULN (or ≤ 5.0 x ULN if liver metastases are present);

9. Aspartame aminotransferase (AST) ≤ 3.0 x ULN (or ≤ 5.0 x ULN if liver metastases are present);

10. Potassium ≥ 1.0 x lower limit of normal (LLN),

11. Magnesium ≥ 1.0 x LLN,

12. Calcium ≥ 1.0 x LLN,

9. Baseline-corrected QT interval ≤ 450 msec for males and ≤ 470 msec for females,

10. Availability of a representative tumor tissue specimen for exploratory translational research; tumor tissue samples, either formalin- fixed paraffin-embedded (FFPE) tissue block or unstained tumor tissue sections (minimum of 20 positively charged slides) from primary or metastatic site must be submitted to the central laboratory,

11. Registration in a national health care system (PUMa-Protection Universelle Maladie included.

Age and reproductive status

12. Age ≥ 18 years,

13. Women must not be pregnant, breastfeeding, or expecting to conceive during the study,

14. Reproductive status:

1. Women of childbearing potential (WOCBP) must have a negative serum pregnancy test within 72 hours prior to the start of study drug,

2. WOCBP must agree to use an adequate method of contraception or birth control for the duration of study treatment and 5 months (nivolumab), 9 months (oxaliplatin), 6 months (5-FU) or at least 1 month (EXL01) of the patient's last dose of the study drug,

3. Males who are fertile and sexually active with WOCBP must agree to follow instructions for method(s) of contraception for the duration of study treatment and 6 months (nivolumab, oxaliplatin, or 5-FU) or at least 1 month (EXL01) after the last dose of study treatment. In addition, males must be willing to refrain from sperm donation during this time,

Exclusion Criteria

Target Disease Exceptions

1. Known HER-2 positive status or unknown HER-2 status before inclusion,

2. Active brain metastases or known history of leptomeningeal carcinomatosis,

3. Ascites, which cannot be controlled with appropriate interventions,

4. Prior malignancy active within the previous 3 years except for locally curable cancers that have been apparently cured, such as basal or squamous cell skin cancer, superficial bladder cancer, or carcinoma in situ of the prostate, cervix, or breast,

5. Active, known, or suspected autoimmune disease; type I diabetes mellitus, hypothyroidism only requiring hormone replacement, skin disorders (such as vitiligo, psoriasis, or alopecia) not requiring systemic treatment, or conditions not expected to recur in the absence of an external trigger are permitted,

6. Interstitial lung disease that is symptomatic or may interfere with the detection or management of suspected treatment-related pulmonary toxicity,

7. Prior treatment with an anti-PD(L)1, anti-LAG-3, or anti-CTLA-4 antibody, or any other antibody or drug specifically targeting T-cell co- stimulation or immune checkpoint pathways, including prior therapy with anti-tumor vaccines or other immuno-stimulatory antitumor agents,

8. Condition requiring systemic treatment with either corticosteroids (>10 mg daily prednisone equivalent) or other immunosuppressive medications within 14 days (2 weeks) of randomization. Inhaled or topical steroids, and adrenal replacement steroid doses >10 mg daily prednisone equivalent, are permitted prior to randomization in the absence of active autoimmune disease,

9. Persistence of toxicity (The National Cancer Institute Common Terminology Criteria for Adverse Event [NCI CTCAE] v 5.0) grade >1 related to prior anticancer treatments,

10. Major surgery within 28 days (4 weeks) prior to first dose of study treatment, Note: Participants who had surgery >4 weeks prior to screening must have recovered adequately from any toxicity and/or complications from the surgery or trauma prior to starting study intervention.

11. Concomitant unplanned antitumor therapy (e.g., chemotherapy, molecular targeted therapy, radiotherapy, immunotherapy),

12. GI obstruction, poor oral intake, or difficulty in taking oral medication or difficulties in swallowing; nasogastric tubes are not permitted,

13. Known GI malabsorption,

14. Is currently participating in or has participated in a study with an investigational compound within 28 days prior to the first dose of study treatment, Note: Participants who have entered the follow-up phase of an investigational study may participate so long as it has been at least 3 months since the last dose of the previous investigational agent,

15. Prior allogeneic bone marrow transplantation or prior solid organ transplantation,

16. Fecal microbiota transplant within 3 months prior to screening, Note: Patients must have recovered adequately from the toxicity and/or complications from the treatment prior to starting study intervention.

17. Current probiotics administration, or planned probiotics administration during treatment course is not allowed, Note: The following therapies should be avoided during the study; however, they are not prohibited if, in the assessment of the

Investigator, they are required for clinical management:

• Nonsteroidal anti-inflammatories,
• Antacids,
• Proton-pump inhibitors.

18. Excessive alcohol intake: moderate consumption, defined as no more than 1 drink per day for women and no more than 2 drinks per day for men, is permitted,

19. Known allergy and/or hypersensitivity to any component or excipients of study treatments (nivolumab, EXL01), any other live pro- biotherapeutic product, and/or to soybean or soy-containing products,

20. Known history or newly diagnosed GI parasitic infection within 3 months prior to screening, Note: Patients must have recovered adequately from the toxicity and/or complications from the treatment prior to starting study intervention,

22. Active or chronic hepatitis B virus (HBV), hepatitis C virus (HCV) and/or human immunodeficiency virus infection (HIV 1/2 antibodies).

Participants are eligible if they:

• Have controlled HCV load defined as undetectable hepatitis C RNA by polymerase chain reaction either spontaneously or in response to a successful prior course of anti-hepatitis C therapy,
• Have received HBV vaccination with only anti-HBs positivity and no clinical signs of hepatitis,
• Are HBV surface antigen (HBsAg)- and anti- Hepatitis B core antibody (HBc)+ (i.e., those who have cleared HBV after infection),
• Are HBsAg+ with chronic HBV infection (lasting 6 months or longer) and meet conditions below:

• HBV DNA viral load <100 IU/mL,
• Have normal transaminase values, or, if liver metastases are present, abnormal transaminases, with a result of AST/ALT <3 × ULN, which are not attributable to HBV infection,
• Start or maintain antiviral treatment if clinically indicated as per the investigator,

23. Any (attenuated) live vaccine use within 28 days (4 weeks) prior to randomization, while in the study; live vaccines include, but are not limited to, the following: yellow fever, varicella, shingles, measles, mumps, rubella, tuberculosis, rotavirus, influenza,

24. Ongoing or concomitant use of the antiviral drug sorivudine or its chemically related analogs, such as brivudine,

25. Dihydropyrimidine dehydrogenase deficiency (DPD; uracilemia dosage >16 ng/ml), Uracilemia dosing results must be available before inclusion,

26. Any condition that, in the opinion of the investigator, would interfere with evaluation of the investigational product or interpretation of the patient's safety or study results,

28. Known potentially serious infection, according to the SmPC of 5-FU

29. Has clinically significant active heart disease or myocardial infarction within 6 months given the cardiotoxicity of 5-FU, according to the SmPC of 5-FU,

30. Known history of hypersensitivity to 5-FU, oxaliplatin, or leucovorin, or to any of their excipients, according to the SmPCs of these products.

31. Impossibility of submitting to the medical follow-up of the study for geographical, social, or psychiatric illness,

32. Patient under a legal protection regime (guardianship, curatorship, judicial safeguard) or administrative decision or incapable of giving his/her consent.

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

GERCOR - Multidisciplinary Oncology Cooperative Group

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Romain Cohen, MD

Role: PRINCIPAL_INVESTIGATOR

Saint-Antoine Hospital

Locations

Institut de Cancerologie de L'Ouest Paul Papin

Angers, , France

Site Status: NOT_YET_RECRUITING

Clinique Sainte Catherine

Avignon, , France

Site Status: RECRUITING

Centre Hospitalier Universitaire Jean Minjoz

Besançon, , France

Site Status: RECRUITING

Institut Bergonie

Bordeaux, , France

Site Status: NOT_YET_RECRUITING

Centre Hospitalier Universitaire Morvan

Brest, , France

Site Status: NOT_YET_RECRUITING

Centre Francois Baclesse

Caen, , France

Site Status: NOT_YET_RECRUITING

Centre Hospitalier de Cholet

Cholet, , France

Site Status: RECRUITING

Centre Hospitalier Universitaire Clermont Ferrand - Site Estaing

Clermont-Ferrand, , France

Site Status: NOT_YET_RECRUITING

Centre Hospitalier Henri Mondor

Créteil, , France

Site Status: NOT_YET_RECRUITING

Centre Georges Francois Leclerc

Dijon, , France

Site Status: RECRUITING

Centre Hospitalier Universitaire Grenoble Alpes - Site Nord - Hopital Michallon

La Tronche, , France

Site Status: NOT_YET_RECRUITING

Centre Hospitalier Universitaire de Lille

Lille, , France

Site Status: RECRUITING

Hopital Leon Berard

Lyon, , France

Site Status: RECRUITING

Hopital Prive Jean Mermoz

Lyon, , France

Site Status: NOT_YET_RECRUITING

Hopital La Timone

Marseille, , France

Site Status: NOT_YET_RECRUITING

Centre Hospitalier Universitaire de Montpellier

Montpellier, , France

Site Status: NOT_YET_RECRUITING

Centre Hospitalier Universitaire Nantes - Hopital Hotel Dieu

Nantes, , France

Site Status: NOT_YET_RECRUITING

Centre Antoine Lacassagne

Nice, , France

Site Status: RECRUITING

Centre Hospitalier Cochin

Paris, , France

Site Status: NOT_YET_RECRUITING

Groupe Hospitalier Diaconesses Croix Saint-Simon

Paris, , France

Site Status: NOT_YET_RECRUITING

Hopital Europeen Georges Pompidou

Paris, , France

Site Status: NOT_YET_RECRUITING

Hopital Saint Antoine

Paris, , France

Site Status: RECRUITING

Hopital Saint-Louis

Paris, , France

Site Status: NOT_YET_RECRUITING

Institut Curie

Paris, , France

Site Status: NOT_YET_RECRUITING

Institut Gustave Roussy

Paris, , France

Site Status: NOT_YET_RECRUITING

Institut Mutualiste Montsouris

Paris, , France

Site Status: NOT_YET_RECRUITING

Centre Hospitalier Universitaire de Poitiers - Hopital de La Miletrie

Poitiers, , France

Site Status: RECRUITING

Centre Hospitalier Universitaire Reims Hopital Robert Debre

Reims, , France

Site Status: RECRUITING

Institut Jean Godinot

Reims, , France

Site Status: NOT_YET_RECRUITING

Centre Hospitalier Universitaire de Rennes

Rennes, , France

Site Status: NOT_YET_RECRUITING

Hopital D'Instruction Des Armées Bégin

Saint-Mandé, , France

Site Status: NOT_YET_RECRUITING

Centre Hospitalier Saint-Malo

St-Malo, , France

Site Status: NOT_YET_RECRUITING

Institut de Cancerologie Strasbourg Europe

Strasbourg, , France

Site Status: NOT_YET_RECRUITING

Centre Hospitalier Universitaire Tours - Hopital Trousseau

Tours, , France

Site Status: NOT_YET_RECRUITING

CENTRE HOSPITALIER REGIONAL UNIVERSITAIRE DE NANCY Site Brabois

Vandœuvre-lès-Nancy, , France

Site Status: NOT_YET_RECRUITING

Hopital Paul Brousse

Villejuif, , France

Site Status: RECRUITING

Medipole Hopital Mutualiste Lyon-Villeurbanne

Villeurbanne, , France

Site Status: NOT_YET_RECRUITING

Countries

France

Central Contacts

Marie-Line GARCIA LARNICOL, MD

Role: CONTACT

marie-line.garcia-larnicol@gercor.com.fr

+33 (01) 40 29 85 04

Facility Contacts

Sandrine HIRET, MD

Role: primary

Clemence TOULLEC, MD

Role: primary

Angelique VIENOT, MD

Role: primary

Simon PERNOT, MD

Role: primary

Estelle Dhamelincourt, MD

Role: primary

Aurélie PARZY, MD

Role: primary

Thonas CENTRE HOSPITALIER DE CHOLET, MD

Role: primary

Marine JARY, MD

Role: primary

Charlotte Fenioux, MD

Role: primary

Francois GHIRINGHELLI, MD

Role: primary

Gaël ROTH, MD

Role: primary

Anthony TURPIN, MD

Role: primary

Clelia COUTZAC, MD

Role: primary

Pascal ARTRU, MD

Role: primary

Laëtitia DAHAN, MD

Role: primary

Eric ASSENAT, MD

Role: primary

Tamara MATYSIAK-BUDNIK, MD

Role: primary

Ludovic Evesque, MD

Role: primary

Anna PELLAT, MD

Role: primary

Olivier DUBREUIL, MD

Role: primary

Aziz Zaanan, MD

Role: primary

Romain COHEN, MD

Role: primary

Thomas APARICIO, MD

Role: primary

Cindy NEUZILLET, MD

Role: primary

Cristina SMOLENSCHI, MD

Role: primary

Emilie SOULARUE, MD

Role: primary

David TOUGERON, MD

Role: primary

Olivier BOUCHE, MD

Role: primary

Damien BOTSEN, MD

Role: primary

Astrid LIEVRE, MD

Role: primary

Julie LAVOLE, MD

Role: primary

Romain DESGRIPPES, MD

Role: primary

Meher BEN ABDELGHANI, MD

Role: primary

Elise DOUARD TOURNET, MD

Role: primary

Marie MULLER, MD

Role: primary

Pascal Hammel, MD

Role: primary

Mathieu SARABI, MD

Role: primary

Other Identifiers

BIG G-122 PRODIGE 94

Identifier Type: -

Identifier Source: org_study_id