Safety of PiggyBac Transposon CAR T-cells Targeting CD-19 in Refractory Lupus.

NCT ID: NCT06150651

Last Updated: 2024-02-12

Basic Information

• Recruitment Status: RECRUITING
• Clinical Phase: PHASE1
• Total Enrollment: 6 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2023-12-01
• Study Completion Date: 2025-11-30

Brief Summary

A Phase 1 clinical trial to evaluate the safety and efficacy of PiggyBac transposon-mediated Chimeric Antigen Receptor(CAR) T-cells targeting CD19 in refractory Systemic Lupus Erythematosus (SLE) patients who have not responded to standard immunosuppressive treatments.

Detailed Description

This is a single-institution phase I study in adults with refractory SLE. Autologous Peripheral Blood Mononuclear Cells will be transduced with a chimeric antigen receptor targeting the B-cell surface antigen CD19 using the PiggyBac Transposon system. Subjects will receive a conditioning lymphodepletion chemotherapy regimen of fludarabine and cyclophosphamide, followed by the infusion of 1x10^6 cells/kg CD-19 CAR T-cells. Subjects will be evaluated post-treatment for toxicity, SLE disease activity, and the persistence of CAR-expressing T cells in vivo.

Conditions

SLE (Systemic Lupus)

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

CAR T-cell therapy

Subjects will receive a conditioning lymphodepletion chemotherapy regimen of fludarabine and cyclophosphamide, followed by the infusion of 1x10\^6 cells/kg CD-19 CAR-T cells.

Group Type: EXPERIMENTAL

CAR T-cell therapy

Intervention Type: OTHER

1x10\^6 cells/kg CD-19 CAR-T cells

Interventions

CAR T-cell therapy

1x10\^6 cells/kg CD-19 CAR-T cells

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

1. Age between 18 and 60 years.

2. Diagnosis of Systemic Lupus Erythematosus (SLE), as defined by the American College of Rheumatology (ACR) 1997 criteria, The Systemic Lupus International Collaborating Clinics (SLICC) criteria, or the European Alliance of Associations for Rheumatology (EULAR)/ACR classification.

3. Refractory SLE, defined by one or more of the following:

3.1 Persistently active SLE requiring ongoing maintenance therapy (if not contraindicated) with:

• Antimalarial drug.
• Either mycophenolate (minimum daily dose of 1500 mg) or azathioprine (minimum daily dose of 1.5 mg/kg).
• Patients must also need a minimum daily dose of 7.5 mg prednisolone for lower disease activity maintenance, or have a SLEDAI score of 8 or higher.

3.2 Biopsy-proven proliferative lupus nephritis after two standard induction therapies, including intravenous cyclophosphamide (cumulative dose of at least 1.5 g) and mycophenolate mofetil (administered for a minimum of 3 months), unless contraindicated.

3.3 Worsening of biopsy-proven lupus nephritis (activity index > 6 and chronicity index < 6 within 6 months), indicated by increased proteinuria and/or decreased estimated glomerular filtration rate, despite treatment with high-dose corticosteroids (prednisolone at least 0.7 mg/kg/day or equivalent) and either mycophenolate mofetil or cyclophosphamide for a minimum of 14 days.

4. Ability to understand and willingness to sign a written informed consent document.

5. Participants of child-bearing or child-fathering potential must agree to practice birth control from enrollment until four months after receiving CAR T-cell infusion.

Exclusion Criteria

1. Pregnant or breastfeeding women.

2. History of active malignancy, excluding non-melanoma skin cancer and carcinoma in situ (e.g., cervix, bladder, breast).

3. History of vital organ transplantation (e.g., heart, lung, kidney, liver) or hematopoietic stem cell/bone marrow transplantation.

4. Uncontrolled intercurrent illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, pulmonary abnormalities, cirrhosis, or psychiatric illness/social situations that limit compliance with study requirements.

5. Any other clinically significant disease history or current disease that, in the judgment of the research physician, may pose a risk to the safety of the subjects or interfere with the research procedure, or the evaluation of safety and efficacy.

6. Serologic status indicating active HIV, hepatitis B, or C infection. Participants positive for hepatitis B core antibody, hepatitis B surface antigen, or hepatitis C antibody must have a negative PCR prior to enrollment.

7. History of severe adverse drug reaction to Cyclophosphamide or Fludarabine.

8. Received a live vaccine within 30 days prior to CAR-T cell infusion.

9. eGFR CKD-EPI < 30 ml/min/1.73m^2.

10. Participation in other clinical investigations during the study period.

11. Prior receipt of CAR-T cell therapy outside this protocol.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 60 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

King Chulalongkorn Memorial Hospital

OTHER

Sponsor Role: collaborator

Health Systems Research Institute,Thailand

OTHER_GOV

Sponsor Role: collaborator

Chulalongkorn University

OTHER

Sponsor Role: lead

Responsible Party

Wonngarm Kittanamongkolchai, MD

Principle Investigator

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Wonngarm Kittanamongkolchai, MD

Role: PRINCIPAL_INVESTIGATOR

Chulalongkorn University

Locations

King Chulalongkorn Memorial Hospital

Bangkok, Please Select, Thailand

Site Status: RECRUITING

Countries

Thailand

Central Contacts

Wonngarm Kittanamongkolchai, MD

Role: CONTACT

wonngarm.k@gmail.com

66875995974

Facility Contacts

wonngarm kittanamongkolchai

Role: primary

wonngarm.k@chulacrc.org

6622516704

Other Identifiers

SLE-PB-CD19-CART

Identifier Type: -

Identifier Source: org_study_id