miRNAs in Critical Limb Ischemia (miRNACLI)

NCT ID: NCT06066268

Last Updated: 2025-04-30

Basic Information

• Recruitment Status: RECRUITING
• Total Enrollment: 80 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2016-05-31
• Study Completion Date: 2026-05-31

Brief Summary

For the present study, 40 patients affected by critical limb ischemia of different severity will be enrolled: patients affected by critical limb ischemia undergoing subgenual femoropopliteal bypass; patients suffering from terminal ischemia causing gangrene and therefore undergoing major amputation of the lower limb. Biopsies from the first 20 enrolled patients will be used for miRNA profiling. The total of 40 biopsies obtained from all enrolled patients will instead be used for the validation of the miRNAs identified in the profiling.

The results obtained will be compared with those obtained in 40 control patients affected by infrarenal abdominal aortic aneurysm (AAA) undergoing endovascular exclusion surgery (EVAR) of the AAA without any ischemia of the lower limbs. Also in this case the biopsies of the first 20 control patients enrolled will be used for miRNA profiling. The total of 40 biopsies obtained from all control patients will instead be used for the validation of the miRNAs identified in the profiling. A total of 80 patients (40 cases and 40 controls) will be enrolled.

Detailed Description

The analyzes of the expression of miRNAs and the RISC complex will be carried out on fragments of skeletal muscle taken from different sites depending on whether they are cases or controls.

Cases:

• Patients suffering from CLI undergoing lower limb revascularization via subgenual femoropopliteal bypass: Gastrocnemius muscle biopsies will be taken.
• Patients suffering from terminal CLI and/or gangrene of the lower limb undergoing thigh amputation: biopsies will be taken in different muscle districts. In particular, biopsies of the Sartorius or Vastus lateralis muscle will be taken at the amputation site, both from the proximal muscle belly which remains covering the bone stump, and from the part of the muscle belly sacrificed with the amputated limb. Biopsies of the Gastrocnemius and Tibialis anterior muscles will be taken from the amputated leg.
• Patients suffering from terminal CLI and/or gangrene of the lower limb undergoing leg amputation: biopsies will be taken in different muscle districts. In particular, biopsies of the Gastrocnemius and Tibialis anterior muscles will be carried out at the amputation site, both from the proximal muscle belly which remains covering the bone stump, and from the part of the muscle belly sacrificed with the amputated limb.

Controls:

• The control subjects will be represented by individuals affected by infrarenal abdominal aortic aneurysm (AAA) free from CLI, affected and not affected by type 2 diabetes mellitus, undergoing endovascular exclusion surgery of the AAA (EVAR), similar by age and sex to CLI patients. Sartorius muscle biopsies will be taken.

The analyzes of the expression of circulating miRNAs will be carried out on 2 7 ml tubes of peripheral venous blood, taken before the surgical incision from the same cases and controls described in the previous point.

Conditions

Critical Limb Ischemia

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: RETROSPECTIVE

Study Groups

1) Critical limb ischemia (CLI);

1)Critical limb ischemia (CLI):

• Patients suffering from CLI undergoing lower limb revascularization via subgenual femoropopliteal bypass. muscle biopsies willbe taken.
• Patients suffering from terminal CLI and/or gangrene of the lower limb undergoing thigh amputation. Muscle biopsies will be taken.
• Patients suffering from terminal CLI and/or gangrene of the lower limb undergoing leg amputation. Muscle biopsies will be taken.

Critical limb ischemia: analysis of RNAs

Intervention Type: DIAGNOSTIC_TEST

analysis of miRNA expression levels

2)Controls

2)Controls: patients affected by infrarenal abdominal aortic aneurysm (AAA) free from CLI, affected and not affected by type 2 diabetes mellitus, undergoing endovascular exclusion surgery of the AAA (EVAR), similar by age and sex to CLI patients. Sartorius muscle biopsies will be taken.

Controls: analysis of RNAs

Intervention Type: DIAGNOSTIC_TEST

analysis of miRNAs expression levels

Interventions

Critical limb ischemia: analysis of RNAs

analysis of miRNA expression levels

Intervention Type: DIAGNOSTIC_TEST

Controls: analysis of RNAs

analysis of miRNAs expression levels

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Inclusion Criteria

Cases:

• patients affected by CLI candidates for popliteal exploration surgery and/or subgenual femoropopliteal bypass.
• Patients affected by CLI with irreversible ischemia and/or distal gangrene of the lower limb candidates for major amputation of the lower limb.

Controls:

• patients affected by infrarenal abdominal aortic aneurysm (AAA) undergoing endovascular exclusion surgery of the AAA (EVAR) who are free from CLI, affected and not affected by type 2 diabetes mellitus.

Exclusion Criteria

• Pregnant women
• Patients carriers of genetic diseases
• Patients suffering from malignant neoplasia

• Minimum Eligible Age: 40 Years
• Maximum Eligible Age: 85 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

IRCCS Policlinico S. Donato

OTHER

Sponsor Role: lead

Responsible Party

Fabio Martelli

Dr.

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Fabio Martelli

Role: PRINCIPAL_INVESTIGATOR

IRCCS Policlinico S. Donato

Locations

Irccs Policlinico San Donato

San Donato Milanese, Milan, Italy

Site Status: RECRUITING

Countries

Italy

Central Contacts

Fabio Dr Martelli

Role: CONTACT

fabio.martelli@grupposandonato.it

+39 0226437762

Giovanni Dr Nano, MD

Role: CONTACT

giovanni.nano@grupposandonato.it

+39 0252771

Facility Contacts

FABIO MARTELLI, PhD

Role: primary

fabio.martelli@grupposandonato.it

0252774683

Other Identifiers

miRNACLI

Identifier Type: -

Identifier Source: org_study_id