Rescue of Nephrons With ALE.F02 (RENAL-F02)

NCT ID: NCT06047171

Last Updated: 2025-11-20

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE2

Total Enrollment

80 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-09-07

Study Completion Date

2027-06-30

Brief Summary

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The goal of this clinical trial is to learn if a new drug that might help protect and preserve kidney function in antineutrophil cytoplasmic antibody (ANCA) associated vasculitis (AAV). AAV is a type of autoimmune disease where the body's own immune system attacks itself, and in the case of AAV the body attacks its own small blood vessels. There are many small blood vessels in the kidneys meaning the kidneys are commonly affected in AAV.

The main questions it aims to answer are:

* Is the new drug well tolerated and safe?
* Can the new drug protect and preserve kidney functions when is added to standard therapy?

Researchers will compare the following groups to see how the new drug is tolerated and what effect to preserve kidney tissue has:

* Group A: Standard treatment + ALE.F02 low dose infusions
* Group B: Standard treatment + ALE.F02 high dose infusions
* Group C: Standard treatment + ALE.F02 maximum dose infusions
* Group D: Standard treatment + placebo infusions (inactive substance)

The Treatment period will consist of 24 weeks beginning on Day 1, during which time participants will receive 13 infusions of the study medicine, along with standard therapy for kidney inflammation due to AAV. During the treatment period, participants will have the following assessments:

* A brief physical examination focusing on their skin any pre-existing medical conditions that you have.
* Collection of blood and urine samples for routine safety tests and to assess renal function.
* Collection of blood samples:

* To measure the amount of study medicine in their blood. This is called pharmacokinetics (PK) and it is tested to see how study medicine enters, moves through, and exits the body.
* To test for antidrug antibodies (ADA). To check if their body create antibodies against the study medicine, as this could reduce its effect.
* To measure biomarkers. Biomarkers are specific compounds in the body (can be protein, hormones, or genetic molecules) that indicate normal or abnormal processes taking place in your body and may be a sign of an underlying condition or disease (for example glucose levels are used as biomarker in managing diabetes). They are used to see how well the body responds to a treatment for a disease or condition.
* Collection of urine to measure urine markers of vasculitis/inflammation called biomarkers.
* Urine pregnancy test. A urine pregnancy test is a quick medical test that can tell if a woman is pregnant or not by checking for a hormone which is produced during pregnancy, usually in the urine.
* Chest High Resolution Computed Tomography (HRCT) scan to check whether they have vasculitis affecting their lungs. A CT scan uses special x-ray equipment to take detailed pictures of body tissues and organs to diagnose and monitor conditions in various parts of the body. For the CT scan, they will need to lie still on a table. At Week 24 a second lung CT scan will be performed for participants whose initial scan showed lung vasculitis to see whether your lung vasculitis is getting better or ongoing/worse.

Detailed Description

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Conditions

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Glomerulonephritis Rapidly Progressive

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

TRIPLE

Participants Caregivers Investigators

Study Groups

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Standard treatment + ALE.F02 low dose infusions

Standard treatment + ALE.F02 low dose infusions

Group Type EXPERIMENTAL

ALE.F02

Intervention Type BIOLOGICAL

ALE.F02 is an anti-Claudin-1 (CLDN1) monoclonal antibody (mAb) to selectively target the non-tight junctions (TJ), exposed form of CLDN1. CLDN1 is an integral component of the TJs between cells.

Rituximab

Intervention Type DRUG

Rituximab is a monoclonal antibody that targets cluster of differentiation antigen 20 (CD20), an antigen expressed on the surface of pre-B and mature B-lymphocytes

GlucoCorticoid

Intervention Type DRUG

Glucocorticoids are a class of corticosteroids, which are a class of steroid hormones that bind to the glucocorticoid receptor. Glucocorticoid effects may be broadly classified into two major categories: immunological and metabolic.

Cyclophosphamid

Intervention Type DRUG

Cyclophosphamid is a medication used as chemotherapy and to suppress the immune system

Immunosuppressive Agents

Intervention Type DRUG

Immunosuppressants are drugs that prevent your immune system from attacking healthy cells and tissues by mistake.

Standard treatment + ALE.F02 high dose infusions

Standard treatment + ALE.F02 high dose infusions

Group Type EXPERIMENTAL

ALE.F02

Intervention Type BIOLOGICAL

ALE.F02 is an anti-Claudin-1 (CLDN1) monoclonal antibody (mAb) to selectively target the non-tight junctions (TJ), exposed form of CLDN1. CLDN1 is an integral component of the TJs between cells.

Rituximab

Intervention Type DRUG

Rituximab is a monoclonal antibody that targets cluster of differentiation antigen 20 (CD20), an antigen expressed on the surface of pre-B and mature B-lymphocytes

GlucoCorticoid

Intervention Type DRUG

Glucocorticoids are a class of corticosteroids, which are a class of steroid hormones that bind to the glucocorticoid receptor. Glucocorticoid effects may be broadly classified into two major categories: immunological and metabolic.

Cyclophosphamid

Intervention Type DRUG

Cyclophosphamid is a medication used as chemotherapy and to suppress the immune system

Immunosuppressive Agents

Intervention Type DRUG

Immunosuppressants are drugs that prevent your immune system from attacking healthy cells and tissues by mistake.

Standard treatment + placebo infusions (inactive substance)

Standard treatment + placebo infusions (inactive substance)

Group Type PLACEBO_COMPARATOR

Rituximab

Intervention Type DRUG

Rituximab is a monoclonal antibody that targets cluster of differentiation antigen 20 (CD20), an antigen expressed on the surface of pre-B and mature B-lymphocytes

GlucoCorticoid

Intervention Type DRUG

Glucocorticoids are a class of corticosteroids, which are a class of steroid hormones that bind to the glucocorticoid receptor. Glucocorticoid effects may be broadly classified into two major categories: immunological and metabolic.

Cyclophosphamid

Intervention Type DRUG

Cyclophosphamid is a medication used as chemotherapy and to suppress the immune system

Placebo

Intervention Type DRUG

Drug product that will contain no active ingredient

Immunosuppressive Agents

Intervention Type DRUG

Immunosuppressants are drugs that prevent your immune system from attacking healthy cells and tissues by mistake.

Standard treatment + ALE.F02 maximum dose infusions

Standard treatment + ALE.F02 maximum dose infusions

Group Type EXPERIMENTAL

ALE.F02

Intervention Type BIOLOGICAL

ALE.F02 is an anti-Claudin-1 (CLDN1) monoclonal antibody (mAb) to selectively target the non-tight junctions (TJ), exposed form of CLDN1. CLDN1 is an integral component of the TJs between cells.

Rituximab

Intervention Type DRUG

Rituximab is a monoclonal antibody that targets cluster of differentiation antigen 20 (CD20), an antigen expressed on the surface of pre-B and mature B-lymphocytes

GlucoCorticoid

Intervention Type DRUG

Glucocorticoids are a class of corticosteroids, which are a class of steroid hormones that bind to the glucocorticoid receptor. Glucocorticoid effects may be broadly classified into two major categories: immunological and metabolic.

Cyclophosphamid

Intervention Type DRUG

Cyclophosphamid is a medication used as chemotherapy and to suppress the immune system

Immunosuppressive Agents

Intervention Type DRUG

Immunosuppressants are drugs that prevent your immune system from attacking healthy cells and tissues by mistake.

Interventions

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ALE.F02

ALE.F02 is an anti-Claudin-1 (CLDN1) monoclonal antibody (mAb) to selectively target the non-tight junctions (TJ), exposed form of CLDN1. CLDN1 is an integral component of the TJs between cells.

Intervention Type BIOLOGICAL

Rituximab

Rituximab is a monoclonal antibody that targets cluster of differentiation antigen 20 (CD20), an antigen expressed on the surface of pre-B and mature B-lymphocytes

Intervention Type DRUG

GlucoCorticoid

Glucocorticoids are a class of corticosteroids, which are a class of steroid hormones that bind to the glucocorticoid receptor. Glucocorticoid effects may be broadly classified into two major categories: immunological and metabolic.

Intervention Type DRUG

Cyclophosphamid

Cyclophosphamid is a medication used as chemotherapy and to suppress the immune system

Intervention Type DRUG

Placebo

Drug product that will contain no active ingredient

Intervention Type DRUG

Immunosuppressive Agents

Immunosuppressants are drugs that prevent your immune system from attacking healthy cells and tissues by mistake.

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

1. Are male or female patients ≥18 years of age of any race or ethnicity with a score of \<7 on the Clinical Frailty Scale in the 3 months preceding the onset of RPGN attributed to AAV; Note: The PI should assess the Clinical Frailty Scale based on medical history and interview with the patient
2. Must be willing and able to comply with the study requirements and give informed consent for participation in the study;
3. Must be willing to have a renal biopsy procedure performed no later than prior to study drug administration at the Week 6 Visit; alternatively, a historical biopsy performed up to 45 days prior to the initiation of study drug administration is considered acceptable;
4. Have been newly diagnosed with RPGN within 45 days prior to the initiation of study drug treatment, as demonstrated by the following: - Evidence of loss of renal function with an eGFR of ≤50 mL/min/1.73 m2 and ≥10 mL/min/1.73 m2; and - History of proteinuria of any degree AND/OR hematuria that is temporally associated with the presenting episode of illness and supports the diagnosis of RPGN. Note: The hematuria may be represented by the presence of eumorphic or dysmorphic red blood cells (RBCs) and/or RBC casts. Patients with extrarenal manifestations of ANCA which started prior to RPGN should be discussed with the Medical Monitor and the Sponsor.
5. Are suspected of having RPGN attributed to AAV at Screening based on clinical laboratory diagnostic criteria, including a positive test for an ANCA, ie, anti-myeloperoxidase (MPO) or anti-proteinase 3 (PR3);
6. Have a body weight of ≤130 kg;
7. Female patients must not be pregnant or lactating at Screening and 1 of the following conditions must apply: - Is a female of childbearing potential and agrees to use a highly effective method of birth control during their participation in the study and for at least 5 half-lives or a minimum of 30 days after the last dose of study drug, or as recommended in the Summary of Product Characteristics (SmPC) of any authorized AxMP given as part of background standard of care (SOC) therapy, whichever is longer; or - Is a female of nonchildbearing potential.
8. Female patients must agree not to donate ova for 6 months after the last dose of study drug or as recommended in the SmPC of any authorized AxMP given as part of background SOC therapy, whichever is longer;
9. Male patients must agree to use contraception, in the form of either sexual abstinence or a condom, during their participation in the study and for 90 days after the last dose of study drug or as recommended in the SmPC of any authorized auxiliary medicinal product (AxMP) given as part of background SOC therapy, whichever is longer; and
10. Male patients must agree to abstain from sperm donation during their participation in the study and for 90 days after the last dose of study drug or as recommended in the SmPC of any authorized AxMP given as part of background SOC therapy, whichever is longer.

Exclusion Criteria

1. Have a history of previous RPGN that resolved or ameliorated (ie, the patient had a documented case of RPGN and has suffered a relapse);
2. Have a positive serology test for anti-glomerular basement membrane antibodies;
3. Have evidence of active or latent tuberculosis (TB) determined by a positive (not indeterminate) QuantiFERON®-TB Gold test (or equivalent). In countries where QuantiFERON®-TB Gold test (or equivalent) is not available, radiological criteria, including chest X-ray or computed tomography scan, may alternatively be used;
4. Have a chronic infection that could be exacerbated by RPGN or SOC therapy for RPGN;
5. Have active hepatitis B, hepatitis C, or HIV infection;
6. Have taken any prohibited medications.
7. Have received a course of SOC therapy which exceeds a high-dose prolonged regimen of treatment of ANCA RPGN, such as \>3000 mg of IV methylprednisolone-equipotent glucocorticoids, or \>1 mg/kg/day of oral glucocorticoids (prednisone equivalent) for \>14 days (doses are provided as a guidance for assessment of intensity; patients who received high-dose glucocorticoids should be discussed with and approved by the Medical Monitor and the Sponsor); Note: A course of systemic glucocorticoids attributed to extrarenal manifestation of ANCA should not be included in the assessment of Exclusion Criterion 7 and such occasions should be discussed with the Medical Monitor and the Sponsor. IV methylprednisolone administered as pre-medication for rituximab may be considered as prophylaxis of a potential hypersensitive response to rituximab. This pre-medication is allowed during the course of the study and will not account in the total dose calculation of the IV glucocorticoids threshold of 3000 mg at Screening for eligibility assessment.
8. Have been treated or planned to be treated with avacopan;
9. Have poor venous access;
10. Have participated in an investigational drug or device study and received investigational therapy \<30 days or 5 half lives, whichever is the greater, prior to the first dose of study drug. For biological investigational drugs, the exclusionary period may not be \<90 days prior to the first dose of study drug;
11. Have a history of psoriasis, AD, excessively dry skin or recurrent conjunctivitis that has required treatment prescribed by a physician, scleroderma, vitiligo, or any other active autoimmune dermatological disorder, with the exception of dermatological disorders or skin rashes that are attributable to, or known to be associated with, the underlying diagnosis of AAV, which shall not be exclusionary;
12. Have a diagnosis of systemic lupus erythematosus-AAV overlap syndrome;
13. Have a diagnosis of eosinophilic granulomatosis with polyangiitis;
14. Have evidence of uncontrolled respiratory, cardiac, hepatic, endocrine, central nervous system, or renal disease, unrelated to RPGN or AAV, that the PI believes cannot be readily brought under control, or any other medical condition that in the opinion of the PI renders the patient unsuitable for enrollment and could prevent the successful completion of the study;
15. Have received a live vaccine within 30 days prior to Screening;
16. Have received any vaccine within 7 days of the first dose of study drug other than against influenza or pneumococcal infection;
17. Are employed by the PI or the study site, have direct involvement in the proposed study or other studies under the direction of that PI, or are a family member of the PI or study site personnel;
18. Have not recovered from AEs and/or complications from major surgery prior to the first dose of study drug; Note: The PI should consult with the Medical Monitor and Sponsor to determine if ongoing, significant complications from major surgery are exclusionary.
19. Have active or known history of alcohol or substance abuse within 1 year prior to Day 1/Randomization or have a positive urine drug screen for drugs of abuse at Screening;
20. Have been diagnosed within the preceding 5 years with a malignant neoplastic disease, other than locally invasive cutaneous squamous or basal cell carcinoma;
21. Have alveolar haemorrhage with hypoxia defined by an oxygen saturation \<85% or that requires the use of invasive or noninvasive ventilatory support;
22. Have undergone dialysis within 7 days prior to Screening;
23. Have undergone therapeutic plasma exchange within 7 days prior to the first dose of study drug; or
24. Have known hypersensitivity to the study drug or any of the excipients used in the formulation of the study drug.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Alentis Therapeutics AG

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Vseobecna fakultni nemocnice v Praze

Prague, , Czechia

Site Status RECRUITING

Institut klinicke a experimentalni mediciny

Prague, , Czechia

Site Status RECRUITING

Aalborg University Hospital

Aalborg, , Denmark

Site Status RECRUITING

Aarhus University Hospital

Aarhus, , Denmark

Site Status RECRUITING

Rigshospitalet

Copenhagen, , Denmark

Site Status RECRUITING

Odense University Hospital

Odense, , Denmark

Site Status RECRUITING

Centre Hospitalier Boulogne sur Mer

Boulogne-sur-Mer, Cedex, France

Site Status RECRUITING

CHU Bordeaux - Hopital Pellegrin

Bordeaux, , France

Site Status RECRUITING

CHRU de Brest - Hopital de la Cavale Blanche

Brest, , France

Site Status RECRUITING

CHU Grenoble-Alpes - Hopital Michallon

La Tronche, , France

Site Status RECRUITING

CHU de Nantes - Hotel-Dieu

Nantes, , France

Site Status RECRUITING

CHU de Nimes

Nîmes, , France

Site Status RECRUITING

AP-HP Hopital Pitie-Salpetriere

Paris, , France

Site Status RECRUITING

AP-HP Hopital Cochin

Paris, , France

Site Status RECRUITING

CHU de Toulouse - Hopital Rangueil

Toulouse, , France

Site Status RECRUITING

Centre Hospitalier de Valenciennes

Valenciennes, , France

Site Status RECRUITING

Charite Universitaetsmedizin Berlin

Berlin, , Germany

Site Status RECRUITING

Universitaetsklinikum Koeln (AoeR)

Cologne, , Germany

Site Status RECRUITING

Universitaetsklinikum Carl Gustav Carus Dresden

Dresden, , Germany

Site Status RECRUITING

Universitaetsklinikum Essen

Essen, , Germany

Site Status RECRUITING

Medizinische Hochschule Hannover (MHH)

Hanover, , Germany

Site Status RECRUITING

Universitaetsklinikum Leipzig

Leipzig, , Germany

Site Status RECRUITING

Universitaetsklinikum Schleswig-Holstein - Campus Luebeck

Lübeck, , Germany

Site Status RECRUITING

Klinikum der Ludwig-Maximilians-Universitaet Muenchen

München, , Germany

Site Status RECRUITING

Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII

Bergamo, , Italy

Site Status RECRUITING

Azienda Ospedaliero-Universitaria Careggi

Florence, , Italy

Site Status RECRUITING

IRCCS Ospedale San Raffaele

Milan, , Italy

Site Status RECRUITING

Fondazione Policlinico Universitario A. Gemelli IRCCS - Universita Cattolica del Sacro Cuore

Rome, , Italy

Site Status RECRUITING

APSS Ospedale Santa Chiara di Trento

Trento, , Italy

Site Status RECRUITING

Fundacio Puigvert

Barcelona, , Spain

Site Status RECRUITING

Hospital Universitari de Bellvitge

Barcelona, , Spain

Site Status RECRUITING

Hospital Universitari Arnau de Vilanova

Lleida, , Spain

Site Status RECRUITING

Hospital Universitario 12 de Octubre

Madrid, , Spain

Site Status RECRUITING

Hospital Regional Universitario de Malaga

Málaga, , Spain

Site Status RECRUITING

Linkoping University Hospital

Linköping, , Sweden

Site Status RECRUITING

University Hospital of Umea

Umeå, , Sweden

Site Status RECRUITING

Kantonsspital Baden AG

Baden, , Switzerland

Site Status RECRUITING

Inselspital, Universitaetsspital Bern

Bern, , Switzerland

Site Status RECRUITING

Kantonsspital St. Gallen

Sankt Gallen, , Switzerland

Site Status RECRUITING

Hôpital Fribourgeois-Freiburger Spital

Villars-sur-Glâne, , Switzerland

Site Status WITHDRAWN

Ankara Etlik City Hospital

Ankara, Keçiören, Turkey (Türkiye)

Site Status RECRUITING

Marmara University Medical Faculty Hospital

Istanbul, , Turkey (Türkiye)

Site Status RECRUITING

Kocaeli University Medical School - Internal Medicine - Nephrology

Kocaeli, , Turkey (Türkiye)

Site Status RECRUITING

Erciyes University Faculty of Medicine

Melikgazi, , Turkey (Türkiye)

Site Status RECRUITING

Queen Elizabeth Hospital Birmingham

Birmingham, , United Kingdom

Site Status RECRUITING

Cambridge University - Addenbrooke's Hospital

Cambridge, , United Kingdom

Site Status RECRUITING

Royal Liverpool University Hospital

Liverpool, , United Kingdom

Site Status RECRUITING

Imperial College Healthcare NHS Trust

London, , United Kingdom

Site Status RECRUITING

Royal Preston Hospital

Preston, , United Kingdom

Site Status RECRUITING

Countries

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Czechia Denmark France Germany Italy Spain Sweden Switzerland Turkey (Türkiye) United Kingdom

Central Contacts

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Mohamed Benabed

Role: CONTACT

+41 78 266 19 91

Other Identifiers

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2022-502184-38

Identifier Type: OTHER

Identifier Source: secondary_id

U1111-1286-9095

Identifier Type: OTHER

Identifier Source: secondary_id

ALE.F02.03

Identifier Type: -

Identifier Source: org_study_id

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