Assessing the Effect of a Mineral-enriched Powder on Iron Deficiency in Women of Reproductive Age

NCT ID: NCT05990166

Last Updated: 2025-10-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ACTIVE_NOT_RECRUITING

Clinical Phase

PHASE2

Total Enrollment

150 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-04-27

Study Completion Date

2026-05-30

Brief Summary

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The goal of this clinical trial is to determine if taking a mineral-enriched powder can raise blood iron levels compared to a placebo powder in reproductive-aged women with iron deficiency. The main questions it aims to answer are:

* Does the mineral-enriched powder raise blood iron levels compared to a placebo powder in women when it is taken every day for six months?
* How many participants still have iron deficiency after six months of taking the mineral-enriched powder compared to a placebo powder?

Participants in this clinical trial will drink the mineral-enriched powder containing ferrous iron and zinc sulphate monohydrate or a placebo powder mixed with 1 litre of water daily for six months. The placebo is a look-alike substance that does not contain active ingredients (iron and zinc). Participants will also have to:

* Complete an online "study diary" every two weeks for six months
* Provide a blood sample once a month for six months
* Attend three in-person visits with a researcher, at enrolment (baseline), midline (three months), and endline (six months)
* Complete three sets of online questionnaires (following each in-person visit)
* Complete three sets of dietary assessments (following each in-person visit)
* Provide three stool samples (following each in-person visit)

Detailed Description

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Conditions

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Iron Deficiency Iron Deficiency Anaemia

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Double-blind randomised placebo-controlled trial
Primary Study Purpose

TREATMENT

Blinding Strategy

DOUBLE

Participants Investigators
All participants, researchers, and the study physician will be blinded to the treatment group (active intervention or placebo) to which participants have been randomised. A delegated unblinded staff member will be permitted to unblind participants for safety purposes, if requested by the principal investigators or the study physician.

Study Groups

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Active intervention

Mineral-enriched powder

Group Type EXPERIMENTAL

Mineral-enriched powder

Intervention Type OTHER

Mineral-enriched powder Mineral-enriched powder to allow for at-home fortification of beverages with 5 mg of ferrous iron and 10 mg of zinc sulfate monohydrate

Placebo

Placebo powder

Group Type PLACEBO_COMPARATOR

Placebo powder

Intervention Type OTHER

Placebo powder which is identical to the mineral- enriched powder, but does not contain the active ingredients (ferrous iron and zinc sulfate monohydrate)

Interventions

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Mineral-enriched powder

Mineral-enriched powder Mineral-enriched powder to allow for at-home fortification of beverages with 5 mg of ferrous iron and 10 mg of zinc sulfate monohydrate

Intervention Type OTHER

Placebo powder

Placebo powder which is identical to the mineral- enriched powder, but does not contain the active ingredients (ferrous iron and zinc sulfate monohydrate)

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Non-pregnant and non-lactating
* English speaking with the ability to give informed consent
* 18-35 years of age (inclusive)
* Women who are biologically female
* Iron deficient (SF \>/=12μg/L and \</=30 μg/L). Note: there is currently poor consensus on diagnostic criteria for iron deficiency based on SF concentrations. Current recommendations range from 15 μg/L to 100 μg/L.
* Hb \>/=110 g/L
* Willing and able to agree to the requirements and lifestyle restrictions of this study
* Able to understand and read the questionnaires in English and carry out all study-related procedures
* Located in the greater Ottawa area and a resident of Ontario

Exclusion Criteria

* Individuals who are lactating, pregnant, or planning to become pregnant during the study
* Individuals who are not maintaining adequate birth control measures

* Adequate birth control measures include any option that will adequately prevent pregnancy including: contraceptives, lifestyle choices, complete abstinence, or as a result of other medical methods, procedures, or conditions
* Have a known sensitivity, intolerability, or allergy to any of the study products or their excipients
* Are using vitamin and mineral supplements containing iron and/or zinc
* SF concentrations \<12 μg/L or \>30 μg/L
* Having moderate or severe anaemia (Hb \<109 g/L)
* Expecting to change diet and exercise regimen in the next 6 months
* Are frequent blood donors

* Have donated blood in the last four months
* Donate blood more than two to three times per year
* Had major surgery in the past three months
* Have planned surgery during the course of the study
* History of problematic alcohol or substance use in the 12 months prior to screening (including having been hospitalized for such in an in-patient or out-patient intervention program)
* Use of investigational product(s) in another research study within 30 days prior to the baseline visit or during the study duration
* Using any of the following drugs:

* Antacids or proton pump inhibitors, H2 blockers
* Salicylates, aspirin, corticosteroids, nonsteroidal anti-inflammatory drugs
* Anticoagulants, antiplatelet compounds
* Drugs with known contraindication with iron supplementation or fortification
* Antiviral medications
* Levothyroxine (Synthroid)
* Known medical history of specific conditions including:

* Gastrointestinal disorders: celiac disease, ulcerative colitis, and Crohn's disease
* Gastric cancer and gastric polyps
* Colon cancer
* Diverticular bleeding
* Inflammatory bowel diseases
* Angiodysplasia
* Helicobacter pylori infection
* Hookworm (Ancylostoma duodenale and Necator americanus)
* Defects of hemostasis (hereditary hemorrhagic telangectasia, von Willebrand disease)
* Gastrectomy, duodenal bypass, bariatric surgery
* Erythropoiesis-stimulating agent therapy
* Chronic kidney disease
* Hemochromatosis
* Hemoglobinopathies
* Blood clotting disorder
* Have any other active or unstable medical conditions or use of medications/supplements/ therapies that according to the scientific literature, may adversely affect the participant's ability to complete the study or its measures or pose a significant risk to the participant or the quality of the study data.
Minimum Eligible Age

18 Years

Maximum Eligible Age

35 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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University of Ottawa

OTHER

Sponsor Role collaborator

Hopital Montfort

OTHER

Sponsor Role collaborator

Lucky Iron Fish Enterprise

UNKNOWN

Sponsor Role collaborator

Carleton University

OTHER

Sponsor Role lead

Responsible Party

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Kristin Connor, PhD

Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Kristin Connor, PhD

Role: PRINCIPAL_INVESTIGATOR

Carleton University

Bénédicte Fontaine-Bisson, RD, PhD

Role: PRINCIPAL_INVESTIGATOR

University of Ottawa

Locations

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Carleton University

Ottawa, Ontario, Canada

Site Status

Countries

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Canada

References

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Subar AF, Kirkpatrick SI, Mittl B, Zimmerman TP, Thompson FE, Bingley C, Willis G, Islam NG, Baranowski T, McNutt S, Potischman N. The Automated Self-Administered 24-hour dietary recall (ASA24): a resource for researchers, clinicians, and educators from the National Cancer Institute. J Acad Nutr Diet. 2012 Aug;112(8):1134-7. doi: 10.1016/j.jand.2012.04.016. Epub 2012 Jun 15. No abstract available.

Reference Type BACKGROUND
PMID: 22704899 (View on PubMed)

Bansback N, Tsuchiya A, Brazier J, Anis A. Canadian valuation of EQ-5D health states: preliminary value set and considerations for future valuation studies. PLoS One. 2012;7(2):e31115. doi: 10.1371/journal.pone.0031115. Epub 2012 Feb 6.

Reference Type BACKGROUND
PMID: 22328929 (View on PubMed)

Devlin N, Parkin D, Janssen B. Methods for Analysing and Reporting EQ-5D Data [Internet]. Cham (CH): Springer; 2020. No abstract available. Available from http://www.ncbi.nlm.nih.gov/books/NBK565678/

Reference Type BACKGROUND
PMID: 33347096 (View on PubMed)

Gloor GB, Hummelen R, Macklaim JM, Dickson RJ, Fernandes AD, MacPhee R, Reid G. Microbiome profiling by illumina sequencing of combinatorial sequence-tagged PCR products. PLoS One. 2010 Oct 26;5(10):e15406. doi: 10.1371/journal.pone.0015406.

Reference Type BACKGROUND
PMID: 21048977 (View on PubMed)

Gloor GB, Wu JR, Pawlowsky-Glahn V, Egozcue JJ. It's all relative: analyzing microbiome data as compositions. Ann Epidemiol. 2016 May;26(5):322-9. doi: 10.1016/j.annepidem.2016.03.003. Epub 2016 Apr 2.

Reference Type BACKGROUND
PMID: 27143475 (View on PubMed)

Fernandes AD, Reid JN, Macklaim JM, McMurrough TA, Edgell DR, Gloor GB. Unifying the analysis of high-throughput sequencing datasets: characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis. Microbiome. 2014 May 5;2:15. doi: 10.1186/2049-2618-2-15. eCollection 2014.

Reference Type BACKGROUND
PMID: 24910773 (View on PubMed)

Zierer J, Jackson MA, Kastenmuller G, Mangino M, Long T, Telenti A, Mohney RP, Small KS, Bell JT, Steves CJ, Valdes AM, Spector TD, Menni C. The fecal metabolome as a functional readout of the gut microbiome. Nat Genet. 2018 Jun;50(6):790-795. doi: 10.1038/s41588-018-0135-7. Epub 2018 May 28.

Reference Type BACKGROUND
PMID: 29808030 (View on PubMed)

Abdel Rahman AM, Pawling J, Ryczko M, Caudy AA, Dennis JW. Targeted metabolomics in cultured cells and tissues by mass spectrometry: method development and validation. Anal Chim Acta. 2014 Oct 3;845:53-61. doi: 10.1016/j.aca.2014.06.012. Epub 2014 Jun 12.

Reference Type BACKGROUND
PMID: 25201272 (View on PubMed)

Other Identifiers

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LIFE2022RCT2

Identifier Type: -

Identifier Source: org_study_id

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