Singlet Oxygen Quantification After Skin Exposure to Ultraviolet A (UVA) Light

NCT ID: NCT05865431

Last Updated: 2025-04-27

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

17 participants

Study Classification

INTERVENTIONAL

Study Start Date

2017-03-17

Study Completion Date

2018-02-02

Brief Summary

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The objective of this study is quantification of singlet oxygen species in the skin after exposure to Ultraviolet A (UVA) light

Detailed Description

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Ultraviolet (UV) radiation can lead to skin damage and cancer. Ultraviolet A (UVA) irradiation is 10 to 100 times more abundant in natural sunlight than ultraviolet B (UVB), thus human skin is exposed to more UVA irradiation daily.UVA irradiation is not completely filtered by clothing and it penetrates deeper into the dermis than UVB, potentially causing more damage. It is believed that skin cancer, photo aging, and skin immunomodulation are mediated by the reactive oxygen species (ROS) that are generated in response to UV radiation. Singlet oxygen (1O2) is one type of ROS. The aim of this study is to quantify the level of singlet oxygen generated in the skin after exposure to UVA radiation. Previous studies have used low dose ultraviolet A1(UVA1) irradiation of 20 J/cm2, which mimics exposure to strong sunlight of approximately two hours. This study will use UVA doses equivalent to or less than what humans are exposed to in daily life. Additionally, the aim is to quantify singlet oxygen produced in individuals of various skin types before and after application of sunscreens containing zinc oxide and avobenzone (sun protection factor 30). To our knowledge, there is no method for quantifying singlet oxygen in human skin after exposure to UVA light. This is a novel method that may help the investigators understand further the protective effects of various skin types, as well as sunscreens.

Conditions

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Sun Damaged Skin

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

PREVENTION

Blinding Strategy

NONE

Study Groups

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Zinc Oxide, Avobenzone, No Product

Participants have zinc oxide, avobenzone, and no product applied to 3 different spots on the forearm and singlet oxygen is measured after UV exposure.

Group Type EXPERIMENTAL

Zinc Oxide and Avobenzone

Intervention Type DRUG

Zinc Oxide is applied to one area of the forearm, Avobenzone is applied to another area of the forearm.

Interventions

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Zinc Oxide and Avobenzone

Zinc Oxide is applied to one area of the forearm, Avobenzone is applied to another area of the forearm.

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* Subjects aged 18 years and older

Exclusion Criteria

* Those who are currently smoking or have smoked within the past 3 years.
* Aspirin use
* Multivitamins and supplements that contain vitamin E
* Adults unable to consent
* Individuals who are not yet adults (infants, children, teenagers)
* Pregnant women
* Prisoners
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of California, Davis

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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University of California, Davis

Sacramento, California, United States

Site Status

Countries

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United States

References

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Tewari A, Grage MM, Harrison GI, Sarkany R, Young AR. UVA1 is skin deep: molecular and clinical implications. Photochem Photobiol Sci. 2013 Jan;12(1):95-103. doi: 10.1039/c2pp25323b.

Reference Type BACKGROUND
PMID: 23192740 (View on PubMed)

Langstaff SR. Nursing care study. Rheumatoid arthritis treated with penicillamine. Nurs Times. 1975 Jun 12;71(24):918-9. No abstract available.

Reference Type BACKGROUND
PMID: 166356 (View on PubMed)

Menter JM, Hatch KL. Clothing as solar radiation protection. Curr Probl Dermatol. 2003;31:50-63. doi: 10.1159/000072237.

Reference Type BACKGROUND
PMID: 12882019 (View on PubMed)

Wang F, Smith NR, Tran BA, Kang S, Voorhees JJ, Fisher GJ. Dermal damage promoted by repeated low-level UV-A1 exposure despite tanning response in human skin. JAMA Dermatol. 2014 Apr;150(4):401-6. doi: 10.1001/jamadermatol.2013.8417.

Reference Type BACKGROUND
PMID: 24305962 (View on PubMed)

Sklar LR, Almutawa F, Lim HW, Hamzavi I. Effects of ultraviolet radiation, visible light, and infrared radiation on erythema and pigmentation: a review. Photochem Photobiol Sci. 2013 Jan;12(1):54-64. doi: 10.1039/c2pp25152c.

Reference Type BACKGROUND
PMID: 23111621 (View on PubMed)

Hanson KM, Clegg RM. Observation and quantification of ultraviolet-induced reactive oxygen species in ex vivo human skin. Photochem Photobiol. 2002 Jul;76(1):57-63. doi: 10.1562/0031-8655(2002)0762.0.co;2.

Reference Type BACKGROUND
PMID: 12126308 (View on PubMed)

Provided Documents

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Document Type: Study Protocol

View Document

Other Identifiers

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1042064

Identifier Type: -

Identifier Source: org_study_id

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