Isolation and Characterization of Blastocystis Hominis as Apotential Human Pathogen

NCT ID: NCT05814861

Last Updated: 2023-04-18

Basic Information

• Recruitment Status: UNKNOWN
• Total Enrollment: 72 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2023-12-01
• Study Completion Date: 2024-12-31

Brief Summary

• Detection of the presence of Blastocystis hominis among outpatients presented with GIT symptoms at Assiut University Hospitals in comparison with those in control group.
• Assessment of different culture techniques for detection, and isolation of Blastocystis hominis.
• Molecular identification of Blastocystis hominis using polymerase chain reaction (PCR).

Detailed Description

Blastocystis is a genus of single-celled parasites belonging to a group of organisms that are known as the Stramenopiles . Blastocystis consists of several species, living in the gastrointestinal tracts of humans and farm animals. Many different species can infect humans and would be identified as Blastocystis hominis depending on the sequencing of the gene encoding the 18S rRNA . Among the subtypes recognized, ST1-8 could colonize humans as well as animals.

Infection with B. hominis has a worldwide distribution with infection rate reaches up to 60% in developing countries. Initially B.hominis was accepted as a harmless commensal in the intestinal tract, but recently it was implicated as a pathogen. As it able to produce a cysteine protease which breaks up the mucosal IgA antibody. B.hominis infection has been associated with several gastrointestinal symptoms, Even though most cases are asymptomatic, it is also play a significant role in some chronic gastrointestinal illnesses as irritable bowel syndrome (IBS).

Blastocystis spp. are polymorphic parasites with multiple morphological forms including vacuolar, multivacuolar, granular, ameboid, and cystic forms. At least two out of three infections may be missed during microscopic detection of B.hominis due to their polymorphic nature, and the possibility of confusion with yeast, Cyclospora spp. or fat globules. . Different studies suggested that it multiply rapidly in culture medium and considered it a sensitive method for diagnosis of B.hominis . Recently Molecular diagnosis by polymerase chain reaction (PCR) is considered the most sensitive method for the detection of B.hominis.

Conditions

Blastocystis Hominis Infections

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: CROSS_SECTIONAL

Study Groups

patients presented with GIT symptoms

Fresh Stool samples will be collected in clean sterile cups from patients suffering from gastrointestinal symptoms attending Assiut University Hospitals and examined for detection of Blastocystis hominis

culture and PCR

Intervention Type: DIAGNOSTIC_TEST

Stool samples will be cultured inTwo media :

A-Locke egg serum medium (LE) B- Jones' medium Then Molecular identification of Blastocystis hominis using polymerase chain reaction (PCR).

clinically free persons undergo routine stool analysis for any reasons other than GIT complaint

Fresh Stool samples will be collected in clean sterile cups from clinically free persons under go routine stool examination for any cause other than GIT troubles and examined for detection of Blastocystis hominis

culture and PCR

Intervention Type: DIAGNOSTIC_TEST

Stool samples will be cultured inTwo media :

A-Locke egg serum medium (LE) B- Jones' medium Then Molecular identification of Blastocystis hominis using polymerase chain reaction (PCR).

Interventions

culture and PCR

Stool samples will be cultured inTwo media :

A-Locke egg serum medium (LE) B- Jones' medium Then Molecular identification of Blastocystis hominis using polymerase chain reaction (PCR).

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Inclusion Criteria

• The persons selected for the study are those who are:

1. Suffering from any GIT manifestations, attending outpatient clinics of Internal Medicine and Tropical Medicine Departments at Assiut University Hospitals (case group)

2. clinically free persons undergo routine stool analysis for any reasons other than GIT complaint.(control group)

Exclusion Criteria

• We will exclude any patient who received antibiotic or anti-parasitic drugs the past 4 weeks prior to sample collection.

• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Assiut University

OTHER

Sponsor Role: lead

Responsible Party

Fatma Alzahraa Montaser Ali

Investigator

Responsibility Role: PRINCIPAL_INVESTIGATOR

Central Contacts

Fatma Alzahraa montaser Ali, demonstrator

Role: CONTACT

docfatma490@gmail.com

01060093655

magda mohamed atiya Elnazer, Prof.

Role: CONTACT

Magda.elnazer@med.aun.edu.eg

01143533100

References

A. F. Bafghi, R. Hosseini, H. R. Mollaei, and K. Barzegar, "Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman , Iran," vol. 8, no. 01, pp. 10-16, 2021..

Reference Type: BACKGROUND

S M. Journal, "SOHAG MEDICAL JOURNAL Vol. 21 No.3," vol. 21, no. 3, 2017.

Reference Type: BACKGROUND

M. Q. Khairuzzaman, "No Title血清及尿液特定蛋白检测在糖尿病肾病早期诊断中的意义," vol. 4, no. 1, pp. 64-75, 2016.

Reference Type: BACKGROUND

Other Identifiers

Blastocystis hominis isolation

Identifier Type: -

Identifier Source: org_study_id