PBMC as Biomarkers of Diabetic Cardiomyopathy

NCT ID: NCT05651919

Last Updated: 2025-07-09

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

100 participants

Study Classification

OBSERVATIONAL

Study Start Date

2023-05-23

Study Completion Date

2026-08-23

Brief Summary

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Type 2 diabetes (T2D), especially when associated with metabolic syndrome (MS) is at high risk to develop heart failure with preserved ejection fraction (HFpEF) or heart failure with mildly reduced ejection fraction (HFmrEF), and the specific impact of T2D+MS in cardiac function impairment is usually known as "diabetic cardiomyopathy" (DC). Cardiac remodelling (ie hypertrophy) and subtle myocardial dysfunction are highly prevalent in T2D+MS but not specific enough to predict further HFpEF or HFmrEF. Also, current biomarkers can identify but do not predict HFpEF or HFmrEF in T2D patients; Furthermore, specific biomarkers are needed. Peripheral blood mononuclear cells (PBMC) obtained from a peripheral blood sample can provide insights from calcic and inflammatory pathways, and may identify more specific molecular signatures shared between T2D+MS and HFpEF.

Detailed Description

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Conditions

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Type 2 Diabetes Metabolic Syndrome Heart Failure With Preserved Ejection Fraction Heart Failure With Mildly Reduced Ejection Fraction

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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No-T2D +MS / No-HF (control group)

25 patients without T2D +MS and without Heart Failure will be included in group 1.

Blood test

Intervention Type BIOLOGICAL

During the routine blood test of the patient, 4 more tubes of 4 milliliters (mL) will be collected to make a biological collection of PBMC and plasma for further analysis.

quality of life Questionnaire

Intervention Type BEHAVIORAL

During the routine medical visit, the patient will be asked to fill in a short questionnaire about quality of life.

This is a descriptive system comprises the following five dimensions: mobility, self-care, usual activities, pain/discomfort and anxiety/depression. Each dimension has 3 levels: no problems, some problems, and extreme problems. This decision results into a 1-digit number that expresses the level selected for that dimension. Each patient's health state is referred to in terms of a 5-digit code;

Levels of perceived problems are coded as follows:

* Level 1 is coded as a '1' (indicating no problem)
* Level 2 is coded as a '2' (indicating some problems)
* Level 3 is coded as a '3' (indicating extreme problems) For example, state 11223 indicates no problems with mobility and self-care, no problems with performing usual activities, moderate pain or discomfort and extreme anxiety or depression, while state 11111 indicates no problems.

Diet habits questionnaire

Intervention Type BEHAVIORAL

During the routine medical visit, the patient will be asked to fill in a short questionnaire about his diet habits.

This is an exploratory questionnaire that describes and quantifies the foods (pro- or anti-inflammatory) ingested by patients.

No-T2D +MS / HFpEF or HFmrEF

15 patients without T2D+MS and presenting HFpEF or HFmrEF will be included in group 2

Blood test

Intervention Type BIOLOGICAL

During the routine blood test of the patient, 4 more tubes of 4 milliliters (mL) will be collected to make a biological collection of PBMC and plasma for further analysis.

quality of life Questionnaire

Intervention Type BEHAVIORAL

During the routine medical visit, the patient will be asked to fill in a short questionnaire about quality of life.

This is a descriptive system comprises the following five dimensions: mobility, self-care, usual activities, pain/discomfort and anxiety/depression. Each dimension has 3 levels: no problems, some problems, and extreme problems. This decision results into a 1-digit number that expresses the level selected for that dimension. Each patient's health state is referred to in terms of a 5-digit code;

Levels of perceived problems are coded as follows:

* Level 1 is coded as a '1' (indicating no problem)
* Level 2 is coded as a '2' (indicating some problems)
* Level 3 is coded as a '3' (indicating extreme problems) For example, state 11223 indicates no problems with mobility and self-care, no problems with performing usual activities, moderate pain or discomfort and extreme anxiety or depression, while state 11111 indicates no problems.

Diet habits questionnaire

Intervention Type BEHAVIORAL

During the routine medical visit, the patient will be asked to fill in a short questionnaire about his diet habits.

This is an exploratory questionnaire that describes and quantifies the foods (pro- or anti-inflammatory) ingested by patients.

T2D+MS / no-HF

30 patients presenting T2D +MS and without any type of HF will be included in group 3.

Blood test

Intervention Type BIOLOGICAL

During the routine blood test of the patient, 4 more tubes of 4 milliliters (mL) will be collected to make a biological collection of PBMC and plasma for further analysis.

quality of life Questionnaire

Intervention Type BEHAVIORAL

During the routine medical visit, the patient will be asked to fill in a short questionnaire about quality of life.

This is a descriptive system comprises the following five dimensions: mobility, self-care, usual activities, pain/discomfort and anxiety/depression. Each dimension has 3 levels: no problems, some problems, and extreme problems. This decision results into a 1-digit number that expresses the level selected for that dimension. Each patient's health state is referred to in terms of a 5-digit code;

Levels of perceived problems are coded as follows:

* Level 1 is coded as a '1' (indicating no problem)
* Level 2 is coded as a '2' (indicating some problems)
* Level 3 is coded as a '3' (indicating extreme problems) For example, state 11223 indicates no problems with mobility and self-care, no problems with performing usual activities, moderate pain or discomfort and extreme anxiety or depression, while state 11111 indicates no problems.

Diet habits questionnaire

Intervention Type BEHAVIORAL

During the routine medical visit, the patient will be asked to fill in a short questionnaire about his diet habits.

This is an exploratory questionnaire that describes and quantifies the foods (pro- or anti-inflammatory) ingested by patients.

T2D +MS / HFpEF or HFmrEF

30 patients presenting T2D +MS and HFpEF or HFmrEF will be included in group 4.

Blood test

Intervention Type BIOLOGICAL

During the routine blood test of the patient, 4 more tubes of 4 milliliters (mL) will be collected to make a biological collection of PBMC and plasma for further analysis.

quality of life Questionnaire

Intervention Type BEHAVIORAL

During the routine medical visit, the patient will be asked to fill in a short questionnaire about quality of life.

This is a descriptive system comprises the following five dimensions: mobility, self-care, usual activities, pain/discomfort and anxiety/depression. Each dimension has 3 levels: no problems, some problems, and extreme problems. This decision results into a 1-digit number that expresses the level selected for that dimension. Each patient's health state is referred to in terms of a 5-digit code;

Levels of perceived problems are coded as follows:

* Level 1 is coded as a '1' (indicating no problem)
* Level 2 is coded as a '2' (indicating some problems)
* Level 3 is coded as a '3' (indicating extreme problems) For example, state 11223 indicates no problems with mobility and self-care, no problems with performing usual activities, moderate pain or discomfort and extreme anxiety or depression, while state 11111 indicates no problems.

Diet habits questionnaire

Intervention Type BEHAVIORAL

During the routine medical visit, the patient will be asked to fill in a short questionnaire about his diet habits.

This is an exploratory questionnaire that describes and quantifies the foods (pro- or anti-inflammatory) ingested by patients.

Interventions

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Blood test

During the routine blood test of the patient, 4 more tubes of 4 milliliters (mL) will be collected to make a biological collection of PBMC and plasma for further analysis.

Intervention Type BIOLOGICAL

quality of life Questionnaire

During the routine medical visit, the patient will be asked to fill in a short questionnaire about quality of life.

This is a descriptive system comprises the following five dimensions: mobility, self-care, usual activities, pain/discomfort and anxiety/depression. Each dimension has 3 levels: no problems, some problems, and extreme problems. This decision results into a 1-digit number that expresses the level selected for that dimension. Each patient's health state is referred to in terms of a 5-digit code;

Levels of perceived problems are coded as follows:

* Level 1 is coded as a '1' (indicating no problem)
* Level 2 is coded as a '2' (indicating some problems)
* Level 3 is coded as a '3' (indicating extreme problems) For example, state 11223 indicates no problems with mobility and self-care, no problems with performing usual activities, moderate pain or discomfort and extreme anxiety or depression, while state 11111 indicates no problems.

Intervention Type BEHAVIORAL

Diet habits questionnaire

During the routine medical visit, the patient will be asked to fill in a short questionnaire about his diet habits.

This is an exploratory questionnaire that describes and quantifies the foods (pro- or anti-inflammatory) ingested by patients.

Intervention Type BEHAVIORAL

Eligibility Criteria

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Inclusion Criteria

* Patient coming for a scheduled cardiology or endocrinology visit as part of their follow-up
* Patient coming on an empty stomach for the blood test
* Male or female aged between 40 and 80 inclusive
* Patient without systemic disease and/or without anti-inflammatory treatment
* Patient not objecting to this research
* Patient accepting the conservation of biological samples and the performance of genetic analyzes

Group 1: No-T2D +MS / No-HF (control group)

* Patient without T2D or MS and without heart failure coming to a consultation or day hospital for another reason (e.g. screening for atypical symptom, etc.)

Group 2: No-T2D +MS / HFpEF or HFmrEF

* Patient without T2D or MS
* HFpEF or HFmrEF. diagnosed

Group 3: T2D+MS / no-HF

* Patient diagnosed with T2D+MS

Group 4: T2D +MS / HFpEF or HFmrEF

* Patient diagnosed with T2D and MS
* HFpEF or HFmrEF. diagnosed


* History (ATCD) of cardiovascular disease (valvular (more than moderate valve disease), radiation, post-cardiotoxic chemotherapy, amyloidosis, etc.) other than HFpEF or HFmrEF
* Atrial fibrillation or permanent flutter ( paroxysmal AF is not a non-inclusion criterion: the rhythm must be sinus at the time of the ultrasound)
* Systemic disease and/or anti-inflammatory treatment
* ATCD of known coronary artery disease
* Unbalanced hypertension (\>160/100 mmHg)
* Pregnant and lactating women on questioning
* Persons deprived of their liberty by a judicial or administrative decision
* Persons subject to psychiatric care
* Patients subject to a legal protection measure (guardianship, curators)
* Subject participating in another interventional research including a period of exclusion still in progress.
* Chronic kidney disease(GFR\<30ml/min/1.73m2)

Group 1: No-T2D +MS / No-HF (control group)

* Presence of diabetes (whatever the type) and MS
* Presence of heart failure or other known heart disease

Group 2: No-T2D +MS / HFpEF or HFmrEF

* Presence of diabetes (whatever the type) and MS
* No Heart failure
* Left Ventricular Ejection Fraction (LVEF) on ultrasound ≤ 40%

Group 3: T2D+MS / no-HF

* Without diabetes or other type of diabetes than T2D
* Presence of Heart failure or other known coronary artery disease

Group 4: T2D +MS / HFpEF or HFmrEF

* Absence of diabetes or presence of another type of diabetes than T2D
* Absence of Heart failure
* LVEF on ultrasound ≤ 40%
Minimum Eligible Age

40 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Hospices Civils de Lyon

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Hopital Louis Pradel

Bron, , France

Site Status RECRUITING

Countries

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France

Central Contacts

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Hélène THIBAULT, PU,PH

Role: CONTACT

0427856691 ext. +33

Julia CANTERINI

Role: CONTACT

0427856628 ext. +33

Facility Contacts

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Hélène Thibault, PU,PH

Role: primary

04427856691 ext. +33

Other Identifiers

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2022-A02435-38

Identifier Type: OTHER

Identifier Source: secondary_id

69HCL22_0580

Identifier Type: -

Identifier Source: org_study_id

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