The Relationship With Mad Honey Containing Grayanotoxin and Ovary Tissue

NCT ID: NCT05643599

Last Updated: 2025-01-27

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 18 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2022-12-01
• Study Completion Date: 2022-12-31

Brief Summary

For our working group, eighteen healthy Sprague-Dawley female rats were recruited and separated into three groups in an experimental animal laboratory.

Group 1 was given mad honey (n:6) (80 mg/kg); Group 2 was given normal honey (n:6) (80 mg/kg), and Group 3 was the control group (n:6). The groups were given normal and mad honey by oral gavage for 30 days in this study. Rats were anesthetized intramuscularly with 50 mg/kg ketamine and 5 mg/kg xylazine on the 30th day of the study. At the conclusion of the study, female rats in the proestrus phase of the estrous cycle (as indicated by vaginal smear) were sacrificed and their ovarian tissues were placed in neutral formalin solution.

Detailed Description

Honey poisoning is caused by consuming honey produced by bees that feed on Rhododendron family plants. Rhododendron poisoning goes by names such as mad honey poisoning or grayanotoxin poisoning. The purpose of this study is to determine the effects of grayanotoxin in mad honey on ovarian tissue folliculogenesis in terms of cell death and nitric oxide expression. Three groups of 18 female Sprague-Dawley rats were formed. The first group received mad honey (80 mg/kg), the second group received normal honey (80 mg/kg), and the third group is control. The first and second groups received normal and mad honey by oral gavage for 30 days before being sacrificed under anesthesia The caspase 3 immunostaining group observed a moderate to a strong response, particularly in the granulosa cells of the Graaf follicles in the mad honey group, while the normal honey and control groups observed a weak to moderate reaction. In the mad honey group, immunostaining for caspase8 and caspase 9 revealed a moderate immunoreaction in the granulosa cells of the Graaf follicles, while expression was weak in the normal honey and control groups. The TUNEL approach revealed that the majority of Graaf follicles that exhibited TUNEL positive in the mad honey group and progressed to atresia were Graaf follicles. The iNOS immunoreaction revealed a high level of expression in the mad honey group, particularly in several Graaf follicles. In all three groups, a weak reactivity to eNOS immunostaining was seen in both Graaf follicles and theca external layers. According to the findings of apoptotic and nitric oxide marker expression, it was determined that mad honey may result in an increase in follicular atresia in ovarian follicles when compared to normal honey and control groups.

Conditions

Ovary Disease

Study Design

• Observational Model Type: OTHER
• Study Time Perspective: PROSPECTIVE

Study Groups

Group 1:

Mad honey

Mad Honey

Intervention Type: DIETARY_SUPPLEMENT

mad honey (80 mg/kg); (n:6)

Group 2:

Normal honey

Normal Honey

Intervention Type: DIETARY_SUPPLEMENT

normal honey (80 mg/kg),(n:6)

Group 3:

without intervention

Control

Intervention Type: DIETARY_SUPPLEMENT

Control

Interventions

Mad Honey

mad honey (80 mg/kg); (n:6)

Intervention Type: DIETARY_SUPPLEMENT

Normal Honey

normal honey (80 mg/kg),(n:6)

Intervention Type: DIETARY_SUPPLEMENT

Control

Control

Intervention Type: DIETARY_SUPPLEMENT

Eligibility Criteria

Inclusion Criteria

• healthy rats

Exclusion Criteria

• patient rats
• not eating rats

• Minimum Eligible Age: 8 Weeks
• Maximum Eligible Age: 10 Weeks
• Eligible Sex: FEMALE
• Accepts Healthy Volunteers: Yes

Sponsors

Celal Bayar University

OTHER

Sponsor Role: lead

Responsible Party

Hayrunnisa Yesil Sarsmaz

Asistant Prof.

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Gulsen Gurgen

Role: STUDY_CHAIR

Manisa Celal Bayar University

Suha Turkmen

Role: STUDY_CHAIR

Karadeniz Teknik University

Locations

Hayrunnisa

Yunusemre, Mani̇sa, Turkey (Türkiye)

Countries

Turkey (Türkiye)

Other Identifiers

MCBU_Madhoney

Identifier Type: -

Identifier Source: org_study_id