Assessment of Prognosis Using Peripheral Blood Circulating Tumor DNA in Patients With Cervical Cancer

NCT ID: NCT05602831

Last Updated: 2022-11-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

108 participants

Study Classification

OBSERVATIONAL

Study Start Date

2022-08-10

Study Completion Date

2024-08-31

Brief Summary

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This observational study is conducted to assess the value of using peripheral blood ctDNA to detect dynamic changes in HPV and genetic variants in predicting the prognosis of patients with locally advanced cervical cancer, as compared with traditional imaging and tumor markers.

Detailed Description

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The goal of this study is to assess the prognostic value of ctDNA HPV and gene variant clearance in peripheral blood.

Two cohorts will be enrolled: operable group and radical chemoradiotherapy group.

After enrollment, patients will receive standard treatment and follow-up strategy. Peripheral blood samples will be collected from 2 cohorts of patients before treatment and at different time points after starting treatment. Baseline surgical or puncture tissues will be also obtained.

Peripheral blood ctDNA and baseline tissues will be tested for HPV copy number based on ddPCR and genetic variation based on next-generation sequencing (NGS).

Finally, the correlation of ctDNA HPV and genetic variation clearance with patients prognosis and its value for recurrence monitoring compared to traditional tumor markers and imaging examination will be analyzed.

Conditions

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Locally Advanced Cervical Cancer

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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operable treatment group

After enrollment, patients will receive standard treatment and conventional follow-up strategy.

Peripheral blood samples will be collected before treatment and at different time points after starting treatment.

Baseline surgical tissue will be also obtained.

detect HPV and genetic variants

Intervention Type OTHER

Serum tumor markers and ctDNA HPV and genetic variants will be detected in peripheral blood samples. HPV and genetic variants will be also detected in baseline surgical tissues.

radical chemoradiotherapy group

After enrollment, patients will receive standard treatment and conventional follow-up strategy.

Peripheral blood samples will be collected before treatment and at different time points after starting treatment.

Baseline puncture tissue will be also obtained before radical chemoradiotherapy.

detect HPV and genetic variants

Intervention Type OTHER

Serum tumor markers and ctDNA HPV and genetic variants will be detected in peripheral blood samples. HPV and genetic variants will be also detected in baseline puncture tissues.

Interventions

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detect HPV and genetic variants

Serum tumor markers and ctDNA HPV and genetic variants will be detected in peripheral blood samples. HPV and genetic variants will be also detected in baseline surgical tissues.

Intervention Type OTHER

detect HPV and genetic variants

Serum tumor markers and ctDNA HPV and genetic variants will be detected in peripheral blood samples. HPV and genetic variants will be also detected in baseline puncture tissues.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Patients with initial diagnosis, operable locally advanced cervical cancer, FIGO stage IB2/3-IIA1/2 or initial diagnosis, receive radical chemoradiotherapy locally advanced cervical cancer, FIGO stage IIB-IVA
* Not receiving systemic treatment
* Pathological diagnosis: cervical squamous cell carcinoma
* Aged 18-70 years
* ECOG PS: 0-1
* Patients volunteer to participate in this study and sign the informed consent, with good compliance, and cooperate with the acquisition of tissue samples and blood samples

Exclusion Criteria

* Patients diagnosed with other malignancies within 5 years
* Patients had received previous systemic antitumor therapy
* In the judgment of the investigator, the patients had other factors that might have caused the study to be discontinued
Minimum Eligible Age

18 Years

Maximum Eligible Age

70 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Amoy Diagnostics

INDUSTRY

Sponsor Role collaborator

Fudan University

OTHER

Sponsor Role lead

Responsible Party

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Xiaohua Wu MD

Director of Gynecologic Oncology

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Fudan University Shanghai Cancer Center

Shanghai, , China

Site Status RECRUITING

Countries

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China

Central Contacts

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Hao Wen, MD

Role: CONTACT

+86-021-64175590 ext. 81000

Facility Contacts

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Hao Wen, MD

Role: primary

+862164175590 ext. 81000

Other Identifiers

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LACC-BD-1

Identifier Type: -

Identifier Source: org_study_id

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