Antioxidant Effect of Ozonated Vegetable Oil and Vitamins in Moderately Active Subjects

NCT ID: NCT05592509

Last Updated: 2023-03-27

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

20 participants

Study Classification

INTERVENTIONAL

Study Start Date

2022-09-15

Study Completion Date

2023-03-24

Brief Summary

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The purpose of this pilot study is to evaluate the antioxidant effect of a nutraceutical formulation based on vegetable oil and vitamin complex (vitamin K2 and vitamin B9) in subjects with the same level of physical activity (LAF 1.70-1.99, normally active or moderately active).

Detailed Description

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Study design: The pilot study will enroll 20 subjects with physical activity level LAF 1.70-1.99 (normally active or moderately active).

The 20 subjects will be divided into two groups of 10 subjects following randomization. The first group will take the dietary supplement (three capsules per day) for 60 days, while the second group will take a placebo (three capsules per day) for 60 days.

During the treatment period (60 days), the two groups will undergo follow-ups at days 0, 15, 30 and 60 within which clinical and hematochemical examinations will be conducted. At the end of the 60 days, after the wash-out period (two weeks), as per the cross-over design the group previously taking the dietary supplement will be on placebo (three capsules per day) for 60 days, while the group previously taking placebo will take the dietary supplement (three capsules per day) for 60 days. Again, follow-ups will be at 0, 15, 30 and 60 days.

Conditions

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Oxidative Stress Inflammation

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Twenty subjects will be divided into two groups of 10 subjects following randomization. The first group will take the dietary supplement (three capsules per day) for 60 days, while the second group will take a placebo (three capsules per day) for 60 days.

During the treatment period (60 days), the two groups will undergo follow-ups at days 0, 15, 30 and 60 within which clinical and hematochemical examinations will be conducted as per Table 2. At the end of the 60 days, after the wash-out period (two weeks), as per the cross-over design the group previously taking the dietary supplement will be on placebo (three capsules per day) for 60 days, while the group previously taking placebo will take the dietary supplement (three capsules per day) for 60 days. Again, follow-ups will be at 0, 15, 30 and 60 days.
Primary Study Purpose

PREVENTION

Blinding Strategy

NONE

Study Groups

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Supplement

This arm will take dietary supplement

Group Type ACTIVE_COMPARATOR

Ozonized vegetable oil with vitamines

Intervention Type DIETARY_SUPPLEMENT

description: A single capsule is composed by ozonized vegetable oil (75 mg), vitamin K2 (20 mcg), vitamin B9 (130 mcg). The posology is three capsules/day.

The time of administration is two months.

Placebo

Placebo will take the placebo

Group Type PLACEBO_COMPARATOR

Ozonized vegetable oil with vitamines

Intervention Type DIETARY_SUPPLEMENT

description: A single capsule is composed by ozonized vegetable oil (75 mg), vitamin K2 (20 mcg), vitamin B9 (130 mcg). The posology is three capsules/day.

The time of administration is two months.

Interventions

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Ozonized vegetable oil with vitamines

description: A single capsule is composed by ozonized vegetable oil (75 mg), vitamin K2 (20 mcg), vitamin B9 (130 mcg). The posology is three capsules/day.

The time of administration is two months.

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Subjects of both sexes;
* Aged between 18 and 60 years, naïve to taking antioxidant supplements;
* Absent of chronic diseases and current therapies;
* willing and able to understand and sign an informed consent;
* willing to follow a dietary pattern developed according to the LARN (Reference Intake Levels of Nutrients and Energy for the Italian Population) guidelines that establish reference intake levels for Average Energy Requirement (AR) and Macronutrients (Carbohydrates, Lipids and Protein) for the Italian adult population \[LARN Tables\*\];
* Hematobiochemical examinations in normal range: blood count, lipid status, renal and liver function, inflammatory status (Tnfα, C-reactive protein, ESR), glycemic profile (Fasting blood glucose, HbA1C, insulinemia, Homa Index);
* BMI in the normal range (18-24.99);
* Physical activity level LAF 1.70-1.99 (normally active or moderately active)

Exclusion Criteria

* Chronic diseases (chronic renal failure, chronic hepatocellular failure, autoimmune diseases, chronic inflammatory bowel disease, diabetes mellitus, end-stage neoplasms, symptomatic chronic ischemic heart disease)
* Severe hypertension;
* High-grade hypercholesterolemia;
* Age \< 18 years;
* Poor compliance;
* Intake of dietary supplements containing antioxidants;
* Untreated hypothyroidism;
* Pregnant and lactating women;
* Underweight subjects (BMI ≤18.49);
* Overweight subjects (25≤ BMI ≤30);
* Subjects with obesity (BMI ≥30);
* Subjects with different levels of physical activity: very active (LAF 2.00-2.40) and sedentary (LAF 1.40-1.69).
Minimum Eligible Age

18 Years

Maximum Eligible Age

60 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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S&R Farmaceutici spa

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Emanuela Floridi, Doctor

Role: PRINCIPAL_INVESTIGATOR

Crabion srl

Locations

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Crabion srl

Corciano, Perugia, Italy

Site Status

Countries

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Italy

References

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Other Identifiers

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ANTIOX001

Identifier Type: -

Identifier Source: org_study_id

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