" Effect of Single-nucleotide Polymorphism of CD40 Gene rs1883832 C/T on the Risk of Immune Thrombocytopenic Purpura "

NCT ID: NCT05585944

Last Updated: 2022-10-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

80 participants

Study Classification

INTERVENTIONAL

Study Start Date

2022-11-01

Study Completion Date

2023-05-01

Brief Summary

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Immune thrombocytopenia is an acquired autoimmune disease, in which platelets are opsonized by auto-antibodies and destroyed by phagocytic cells .Genetic polymorphisms in the immune mediators have been suggested to play a pivotal role in the pathogenesis of many autoimmune disorders . Several genetic polymorphisms of the immune system genes have been described in ITP such as interleukins, tumor necrosis factors (TNF) alpha and beta, and interferon-gamma., These polymorphisms were found to be associated with an increased risk of ITP progression or exacerbation .CD40 is a co-stimulatory 4348 kDa glycoprotein molecule composed of 277 amino acid residues which belongs to the tumor necrosis family. It is encoded by a gene which is located at chromosome 20q11-13, expressed mainly on antigen presenting cells (APCs), some non-immune cells and tumors.Antinuclear antibodies (ANA) is a collective term for a large and heterogeneous group of circulating autoantibody. Reflecting their clinical importance, ANA are diagnostic, prognostic or classification criteria for many autoimmune diseases.

Detailed Description

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Conditions

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Immune Thrombocytopenic Purpura

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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control

healthy participants

Group Type ACTIVE_COMPARATOR

Genotyping of rs1883832C/T related single-nucleotide polymorphism

Intervention Type DIAGNOSTIC_TEST

Genotyping of rs1883832C/T related single-nucleotide polymorphism by real time pcr

cases

cases of immune thrombocytopenic purpura

Group Type ACTIVE_COMPARATOR

Genotyping of rs1883832C/T related single-nucleotide polymorphism

Intervention Type DIAGNOSTIC_TEST

Genotyping of rs1883832C/T related single-nucleotide polymorphism by real time pcr

Interventions

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Genotyping of rs1883832C/T related single-nucleotide polymorphism

Genotyping of rs1883832C/T related single-nucleotide polymorphism by real time pcr

Intervention Type DIAGNOSTIC_TEST

Other Intervention Names

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ANA test.

Eligibility Criteria

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Inclusion Criteria

* sign an informed written consent from the patients or their parents
* patient with newly diagnosed ITP
* patient age \> 1 year and \< 65 years and both sexes are included.

Exclusion Criteria

* Refusal to sign an informed written consent
* patient with persistent ITP, patient with secondary immune thrombocytopenia
* patient age \< 1 year or \> 65 years.
Minimum Eligible Age

1 Year

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Sohag University

OTHER

Sponsor Role lead

Responsible Party

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Nagwa Mohamed Aboelmahasen

assisstant lecturer of clinical and chemical pathology department, Sohag university hospital

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Sohag University hospitals

Sohag, , Egypt

Site Status

Countries

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Egypt

Central Contacts

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nagwa M Aboelmahasen, assisstant lecturer

Role: CONTACT

01008831102

Elham O Hamed, Professor

Role: CONTACT

Facility Contacts

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Magdy M Amin, Professor

Role: primary

References

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Behzad MM, Asnafi AA, Jalalifar MA, Moghtadaei M, Jaseb K, Saki N. Cellular expression of CD markers in immune thrombocytopenic purpura: implications for prognosis. APMIS. 2018 Jun;126(6):523-532. doi: 10.1111/apm.12853.

Reference Type BACKGROUND
PMID: 29924452 (View on PubMed)

Chen JM, Guo J, Wei CD, Wang CF, Luo HC, Wei YS, Lan Y. The association of CD40 polymorphisms with CD40 serum levels and risk of systemic lupus erythematosus. BMC Genet. 2015 Oct 16;16:121. doi: 10.1186/s12863-015-0279-8.

Reference Type BACKGROUND
PMID: 26474561 (View on PubMed)

Favaloro EJ. How to Generate a More Accurate Laboratory-Based International Normalized Ratio: Solutions to Obtaining or Verifying the Mean Normal Prothrombin Time and International Sensitivity Index. Semin Thromb Hemost. 2019 Feb;45(1):10-21. doi: 10.1055/s-0038-1667342. Epub 2018 Aug 22.

Reference Type BACKGROUND
PMID: 30134448 (View on PubMed)

Morodomi Y, Kanaji S, Won E, Ruggeri ZM, Kanaji T. Mechanisms of anti-GPIbalpha antibody-induced thrombocytopenia in mice. Blood. 2020 Jun 18;135(25):2292-2301. doi: 10.1182/blood.2019003770.

Reference Type BACKGROUND
PMID: 32157300 (View on PubMed)

Other Identifiers

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Soh-Med-22-10-21

Identifier Type: -

Identifier Source: org_study_id

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