Safety and Efficacy of ThisCART19A Bridging Hematopoietic Stem Cell Transplantation in Patients With Refractory or Relapsed B-cell Acute Lymphoblastic Leukemia

NCT ID: NCT05576181

Last Updated: 2022-10-12

Basic Information

• Recruitment Status: UNKNOWN
• Clinical Phase: PHASE1
• Total Enrollment: 19 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2022-10-15
• Study Completion Date: 2025-07-22

Brief Summary

This is a a phase 1, open label study to assess the safety and efficacy of ThisCART19 (Allogeneic CAR-T targeting CD19) Bridging Hematopoietic Stem Cell Transplantation in patients with refractory or relapsed B cell acute lymphoblastic leukemia (r/r B-ALL).

Detailed Description

This is a phase 1, single-center, nonrandomized, open-label, dose-escalation study to evaluate the safety and efficacy of ThisCART19A Bridging Hematopoietic Stem Cell Transplantation in patients with CD19 positive r/r B-ALL and identify a treatment regimen most likely to result in clinical efficacy while maintaining a favorable safety profile.

Conditions

Allogeneic, CAR-T, Protein Sequestration, Non-gene Edited

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

ThisCART19A cells infusion and HSCT

In this study, allogeneic anti-CD19 CAR T cell (ThisCART19A) infusion is used to treat patients with refractory or relapsed CD19 positive B cell acute lymphoblastic leukemia.

After patients achieve MRD- remissions through ThisCART19A, they will subsequently receive hematological stem cell transplantations.

Group Type: EXPERIMENTAL

ThisCART19A

Intervention Type: DRUG

ThisCART19A is a new type CAR-T therapy for patients with r/r B-ALL.

Fludarabine Oral Tablet

Intervention Type: DRUG

Fludarabine is used for lymphodepletion.

Cyclophosphamide

Intervention Type: DRUG

Cyclophosphamide is used for lymphodepletion.

VP-16

Intervention Type: DRUG

VP-16 is used for lymphodepletion.

HSCT

Intervention Type: PROCEDURE

Hematological stem cell transplantation

Interventions

ThisCART19A

ThisCART19A is a new type CAR-T therapy for patients with r/r B-ALL.

Intervention Type: DRUG

Fludarabine Oral Tablet

Fludarabine is used for lymphodepletion.

Intervention Type: DRUG

Cyclophosphamide

Cyclophosphamide is used for lymphodepletion.

Intervention Type: DRUG

VP-16

VP-16 is used for lymphodepletion.

Intervention Type: DRUG

HSCT

Hematological stem cell transplantation

Intervention Type: PROCEDURE

Other Intervention Names

etoposide

Eligibility Criteria

Inclusion Criteria

1. All subjects or legal representatives must sign a voluntary letter of consent approved by the IRB in person prior to the commencement of any screening procedure;

2. Patients diagnosed with B-ALL;

3. No gender limitation, Age 14 years to 75 years (both upper and lower limits included);

4. Consistent with the diagnosis of recurrent refractory B-ALL. Recurrence： was defined as the recurrence of lymphoblasts（≥5%） in peripheral blood or bone marrow or extramedullary diseasefor patients who had acquired CR ; Refractory ：was defined as failure to CR or CRi at the end of induction therapy (generally referred to 4-week regimen or Hyper-CVAD regimen);Patients with Ph+ R/R ALL who failed after 2-line TKI treatment, were intolerant to TKI treatment or were not suitable for TKI treatment; The following factors can coexist:

1. Failure to prepare autologous CAR-T (definition: too few autologous lymphocytes [200/ML] or cannot meet the release standard);

2. Experienced treatment with auto car-T/berintoomumab/ CD22 antibody conjugation drugs;

3. ≥100 days after hematopoietic stem cell transplantation;

4. High-risk patients (High risk was defined as a high white blood cell count ≥30×109/L at diagnosis or with poor cytogenetic prognosis);

• Hypodiploid (<44 chromosomes);
• KMT2A rearrangement: t (4;11) or otherwise;
• t (v; 14q32) /IgH
• t (9; 22) (q34; q11.2) or BCR-ABL1
• Complex karyotype (≥5 chromosomal abnormalities);
• BCR-ABL1-like (Ph-like) ALL;
• JAK-STAT (CRLF2r, EPORr, JAK1/2/3r, TYK2r, mutations of SH2B3, IL7r, Jak1/2/3);
• ABL class rearrangements (such as ABL1, ABL2, PDGFRA, PDGFRB, FGFR, etc.)
• Others (NTRKr, FLT3r, LYNr, PTK2Br);
• Intrachromosomal amplification of chromosome 21 (iAMP21);
• t (17; 19): TCF3-HLF fusion;
• Alterations of IKZF1;

5. Extramedullary lesions.

5. The expected survival time is ≥12 weeks;

6. ECOG score 0-2;

7. Adequate bone marrow, renal, hepatic, pulmonary and cardiac function;

8. CD19 was still expressed in leukemia cells in bone marrow, peripheral blood or biopsy tissue by flow cytometry within one month prior to informed consent (after the last treatment).

Exclusion Criteria

1. Allergic to preconditioning measures;

2. Diagnosis of chronic myelogenous leukemia lymphoid blast crisis;

3. Isolated extramedullary relapse;

4. Presence of CNS-3 disease or CNS-2 disease with neurological changes;

5. Imaging confirmed the presence of central nervous system involvement;

6. Severe CNS disorders such as a history of frequent epileptic seizures;

7. Patients with other malignancies other than B-cell malignancies within 5 years prior to screening. Patients with cured skin squamous carcinoma, basal carcinoma, non-primary invasive bladder cancer, localized low-risk prostate cancer, in situ cervical/breast cancer can be recruited.

8. Uncontrollable bacterial, fungal and viral infection during screening;

9. Patients had pulmonary embolism (PE) and/or deep vein thrombosis (DVT) within 3 months prior to enrollment;

10. Had intolerant severe cardiovascular and cerebrovascular diseases and hereditary diseases prior to enrollment;

11. Radiation therapy within 2 weeks prior to lymphodepletion chemotherapy (>30% bone marrow exposure);

12. Active hepatitis B virus (HBV) or hepatitis C virus (HCV) or Human immunodeficiency virus (HIV) or Syphilis infection. HBV-DNA < 2000 IU/mL can be enrolled, but should admitted to use anti-virus drugs such as entecavir, tenofovir, etc, and supervisory the relative indication during the treatment;

13. Vaccinated with influenza vaccine within 2 weeks prior to lymphodepleting chemotherapy (Severe Acute Respiratory Syndrome-Corona virus disease 19 can be included, inactivated, live/non-live adjuvant vaccinations allowed to be included) ;

14. Patients who are receiving Graft versus host disease Hepatitis(GvHD) treatment; Patients without GvHD and who had stopped immunosuppressive drugs for at least 1 month were eligible for inclusion;

15. Women who are in pregnant or lactating, and female subjects or partners who plan to be pregnant within 1 year after cell infusion. Male subjects who plan pregnancy within 1 year after infusion;

16. Any ineligibility conditions considered by the investigator that may increase the risk of the subject or interfere with the results of the study.

• Minimum Eligible Age: 14 Years
• Maximum Eligible Age: 75 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

The First Affiliated Hospital of Zhengzhou University

OTHER

Sponsor Role: collaborator

Fundamenta Therapeutics, Ltd.

INDUSTRY

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Yongping Song, Ph.D

Role: PRINCIPAL_INVESTIGATOR

The First Affiliated Hospital of Zhengzhou University

Central Contacts

Jun Li, Ph.D

Role: CONTACT

jli@ctigen.com

+86-18662604088

Yongping Song, Ph.D

Role: CONTACT

songyongping@medmail.com.cn

+86-13521186987

Other Identifiers

ThisCART19 FT400-007

Identifier Type: -

Identifier Source: org_study_id