EFFECT OF LIMITED VOLUME CONE BEAM COMPUTED TOMOGRAPHY ON MICRONUCLEI CELLS COUNT OF BUCCAL MUCOSA
NCT ID: NCT05532514
Last Updated: 2022-09-08
Study Results
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Basic Information
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UNKNOWN
63 participants
OBSERVATIONAL
2022-08-24
2022-12-15
Brief Summary
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Rationale: Our study aims to detect and establish a correlation between mean micronucleated cell count and nuclear morphometry in exfoliated cells from affected and apparently unaffected mucosa after CBCT radiation and compare them with exfoliated cells from normal appearing mucosa of normal subjects before CBCT. This could prove beneficial in early detection of malignant conversion of buccal mucosa after CBCT and hence improve the prognosis and better treatment outcome of the patient.
RESEARCH QUESTION-what is the effect of CBCT radiations on the no. Of micronucleated cells in the buccal mucosal cells Population- Patients Planned For CBCT Intervention- None Control-None Outcome- change in number of mean micronucleated cell count before and after the CBCT exposure Time- 1 year (Jan 2020- April 2021)
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Detailed Description
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Buccal mucosal cells are under direct radiation exposure in dental radiographic techniques and act as the primary target for radiation-induced damage. Also, it has a highly proliferative epithelial cells and can be easily collected and studied. Micronuclei, an anomaly structure, originates from the chromosome fragments or whole chromosomes that lag behind at anaphase during nuclear division under physical and chemical factors. The micronucleus index has become one of the standard cytogenetic endpoints and biomarkers used in genetic toxicology in vivo or in vitro2. In healthy epithelial tissues these MNi ranges from 0.5 to 2.5 MNi /1000 cells. The studies have shown an increase in their frequency in individuals exposed to radiation or other genotoxic agents.
However, these studies observed the effect on the micronuclei at small and medium FOV, but the radiation induced damage is seen mostly at smaller FOVs which involves high resolution, thereby causing more radiation exposure.
Keeping this in mind, the aim of this study is to observe and correlate the mean micro nucleated cell count of the buccal epithelial cells with the help of PAP smear after CBCT exposure at different voxel sizes (90 and 200) at small FOV. This could prove beneficial and shed light on the cytogenetic damage and malignant conversion potential of buccal mucosa after CBCT.
Conditions
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Study Design
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OTHER
CROSS_SECTIONAL
Study Groups
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GROUP A
exposure in 5x5 fov at 90 voxel size
MICRONUCLEI COUNT
MICRONUCLEI COUNT IN PRECBCT and POSTCBCT EXPOSURE
GROUP B
exposure in 5x5 fov at 200 voxel size
MICRONUCLEI COUNT
MICRONUCLEI COUNT IN PRECBCT and POSTCBCT EXPOSURE
Interventions
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MICRONUCLEI COUNT
MICRONUCLEI COUNT IN PRECBCT and POSTCBCT EXPOSURE
Eligibility Criteria
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Inclusion Criteria
Exclusion Criteria
2\. Subjects who are smokers and alcoholics. 3. Subjects with history of malignancy. 4. Subjects receiving dietary supplementations 5. Any subjects exposed to radiation or other carcinogens in the past one month.
18 Years
60 Years
ALL
Yes
Sponsors
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Postgraduate Institute of Dental Sciences Rohtak
OTHER
Responsible Party
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Locations
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Post Graduate Institute Of Dental Science
Rohtak, Haryana, India
Countries
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Central Contacts
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Facility Contacts
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Ambika Gupta
Role: backup
References
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Arora P, Devi P, Wazir SS. Evaluation of genotoxicity in patients subjected to panoramic radiography by micronucleus assay on epithelial cells of the oral mucosa. J Dent (Tehran). 2014 Jan;11(1):47-55. Epub 2014 Jan 31.
Thomas P, Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, Fenech M. Buccal micronucleus cytome assay. Nat Protoc. 2009;4(6):825-37. doi: 10.1038/nprot.2009.53. Epub 2009 May 7.
da Fonte JB, Andrade TM, Albuquerque RL Jr, de Melo MFB, Takeshita WM. Evidence of genotoxicity and cytotoxicity of X-rays in the oral mucosa epithelium of adults subjected to cone beam CT. Dentomaxillofac Radiol. 2018 Feb;47(2):20170160. doi: 10.1259/dmfr.20170160. Epub 2017 Nov 13.
Popova L, Kishkilova D, Hadjidekova VB, Hristova RP, Atanasova P, Hadjidekova VV, Ziya D, Hadjidekov VG. Micronucleus test in buccal epithelium cells from patients subjected to panoramic radiography. Dentomaxillofac Radiol. 2007 Mar;36(3):168-71. doi: 10.1259/dmfr/29193561.
Belmans N, Gilles L, Virag P, Hedesiu M, Salmon B, Baatout S, Lucas S, Jacobs R, Lambrichts I, Moreels M. Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations. Dentomaxillofac Radiol. 2019 Sep;48(6):20180428. doi: 10.1259/dmfr.20180428. Epub 2019 Apr 5.
Antonio EL, Nascimento AJD, Lima AAS, Leonart MSS, Fernandes A. GENOTOXICITY AND CYTOTOXICITY OF X-RAYS IN CHILDREN EXPOSED TO PANORAMIC RADIOGRAPHY. Rev Paul Pediatr. 2017 Jul-Sep;35(3):296-301. doi: 10.1590/1984-0462/;2017;35;3;00010. Epub 2017 Jul 20.
Other Identifiers
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Dr.Komal Kumia
Identifier Type: -
Identifier Source: org_study_id
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