Type VI Secretion System in Klebsiella Pneumoniae

NCT ID: NCT05516654

Last Updated: 2023-04-12

Basic Information

• Recruitment Status: UNKNOWN
• Total Enrollment: 70 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2022-09-10
• Study Completion Date: 2023-09-30

Brief Summary

Klebsiella pneumoniae (K. pneumoniae) is an important opportunistic pathogen contributing to nosocomial and antimicrobial-resistant infections. The increasing prevalence of infections caused by multidrug-resistant K. pneumoniae has emerged as a major clinical and public health threat, while the serous organ and life-threatening infections caused by highly virulent K. pneumoniae have also emerged( Russo and Marr, 2019; Wyres et al., 2020).

Detailed Description

Both drug-resistant and highly virulent K. pneumoniae have brought major challenges to clinical treatment and stimulated interest in studying K. pneumoniae. However, knowledge of the genomics, ecology, and pathogenicity of K. pneumoniae is relatively limited. Recently, the type VI secretion system (T6SS) was identified as a virulence factor in K. pneumoniae (Martin and Bachman, 2018). Furthermore, K. pneumoniae was found to exploit the T6SS nano-weapon to destroy bacterial competitors and fungi (Storey et al., 2020).

The bacterial Type VI Secretion System (T6SS) is a membrane-attached contractile phage tail that is physically and Mechanistically similar to a membrane-linked intracellular contractile phage tail. T6SS spike and tube elements, as well as anti-bacterial and anti-eukaryotic effectors, are propelled out of predatory T6SS positive cells and into target cells by a fast conformational shift in the structural framework of a sheath protein complex, according to recent research (Kudryashev et al., 2015).

However, the limited studies and lack of information on the T6SS in K. pneumoniae necessitate further exploration to clarify the physiological metabolism and pathogenic information of this clinically important bacterial species.

Conditions

Detection of Type VI Secretion System in K.Pneumoniae; Detection of Relation to Ab Resistance, Biofilm

Study Design

• Observational Model Type: OTHER
• Study Time Perspective: CROSS_SECTIONAL

Interventions

Routine bacteriological cultures

culture of different samples on different culture media as nutrient agar, blood agar, MacConkey agar, EMB agar, TSI

Intervention Type: DIAGNOSTIC_TEST

Identification of the organism and antibiotic sensitivity will be done by VITEK II

Automated identification of the organism and antibiotic sensitivity will be done by VITEK II

Intervention Type: DIAGNOSTIC_TEST

Detection of biofilm formation

Detection of Biofilm formation to the collected organism by tissue culture plate method.

Intervention Type: DIAGNOSTIC_TEST

Molecular detection by PCR

Molecular detection of Type VI Secretion System genes, capsular serotyping( k1, k2), and FimH genes by PCR.

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Inclusion Criteria

• All patients suffering from infections that can be caused by Klebsiella Pneumoniae.

Exclusion Criteria

• Samples diagnosed to have organisms other than Klebsiella Pneumoniae

• Minimum Eligible Age: 12 Weeks
• Maximum Eligible Age: 90 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Sohag University

OTHER

Sponsor Role: lead

Responsible Party

Noha Saber Shafik

lecturer of Medical Microbiology and Immunology, faculty of medicine

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Noha S Shfik, lecturer

Role: PRINCIPAL_INVESTIGATOR

Faculty of medicine, Sohag university

Locations

Faculty Of Medicine

Sohag, , Egypt

Site Status: RECRUITING

Countries

Egypt

Facility Contacts

Noha S Shafik, lecturer

Role: primary

Nohasaber@med.sohag.edu.eg

00201067261504

Nesma A Mohammed, Lecturer

Role: backup

Nesmaaateef@med.sohag.edu.eg

00201006780725

Other Identifiers

Soh-Med-22-8-23

Identifier Type: -

Identifier Source: org_study_id