Expression of Endometrium During Window of Implantation

NCT ID: NCT05478616

Last Updated: 2024-08-05

Basic Information

• Recruitment Status: TERMINATED
• Total Enrollment: 3 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2022-07-01
• Study Completion Date: 2023-12-31

Brief Summary

The aim of this study was to investigate the expression of endometrium during window of implantation between natural cycles and artificial cycles.

Detailed Description

Due to progress of embryo vitrification in recent years, frozen embryo transfer (FET) of in vitro fertilization (IVF) has already been a world trend. The most used two protocols of FET are natural cycles (NCs) and artificial cycles (ACs). Recent studies showed that IVF outcomes of NCs were superior to ACs. We hypothesized that the result may be because of better endometrial receptivity of NCs compared to ACs. In NCs, no hormonal agents were administrated. Thus, endometrial receptivity was not disturbed by hormone and became better. However, there were few studies to explore the issue. Therefore, the aim of this study was to investigate the expression of endometrium during window of implantation (WOI) between NCs and ACs.

The study will be performed from July 1st, 2022 to December 31th, 2023 in our reproductive center. We plan to enroll 60 infertile women who have underwent IVF cycles and would like to receive endometrial receptivity assay. We divide the participants into two groups, NCs and ACs. In NCs, no hormonal agents will be used. We identify ovulation day via serial hormone data and sample endometrium during WOI (LH surge + 7 days). In ACs, first, estrogen will be given for 10~14 days and then transvaginal sonography will be conducted. When endometrial thickness reaches 8 mm at least, progesterone will be prescribed. Endometrium is sampled during WOI (P4 + 5 days). We will record basic characteristics, infertility history, hormone data, ovarian reserve and endometrial thickness of infertile women in both groups. Endometrial samples will be assessed miRNA, implantation factors, uterine NK cells and T cells in both groups.

Conditions

Unrecognized Condition

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: PROSPECTIVE

Study Groups

Natural cycles

Endometrium will be sampled at the day of LH surge + 7 days.

No interventions assigned to this group

Artificial cycles

Endometrium will be sampled at the day of P4 + 5 days.

No interventions assigned to this group

Eligibility Criteria

Inclusion Criteria

• Age: 30-44 years
• BMI: 18~30 kg/m2
• Implantation failure at least two times

Exclusion Criteria

• Primary ovarian insufficiency
• Congenital uterine anomaly
• Severe intrauterine adhesion
• Malignancy
• Donor cycle

• Minimum Eligible Age: 30 Years
• Maximum Eligible Age: 44 Years
• Eligible Sex: FEMALE
• Accepts Healthy Volunteers: No

Sponsors

Kaohsiung Veterans General Hospital.

OTHER

Sponsor Role: lead

Responsible Party

Li-Te Lin

Attending doctor, Department of Obstetrics and Gynecology, Principal Investigator, Assistant professor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Li-Te Lin

Role: STUDY_DIRECTOR

Kaohsiung Veterans General Hospital.

Locations

Kaohsiung Veterans General Hospital

Kaohsiung City, , Taiwan

Countries

Taiwan

References

Teh WT, McBain J, Rogers P. What is the contribution of embryo-endometrial asynchrony to implantation failure? J Assist Reprod Genet. 2016 Nov;33(11):1419-1430. doi: 10.1007/s10815-016-0773-6. Epub 2016 Aug 1.

Reference Type: RESULT

PMID: 27480540 (View on PubMed)

Bellver J, Simon C. Implantation failure of endometrial origin: what is new? Curr Opin Obstet Gynecol. 2018 Aug;30(4):229-236. doi: 10.1097/GCO.0000000000000468.

Reference Type: RESULT

PMID: 29889670 (View on PubMed)

Neves AR, Devesa M, Martinez F, Garcia-Martinez S, Rodriguez I, Polyzos NP, Coroleu B. What is the clinical impact of the endometrial receptivity array in PGT-A and oocyte donation cycles? J Assist Reprod Genet. 2019 Sep;36(9):1901-1908. doi: 10.1007/s10815-019-01535-5. Epub 2019 Jul 27.

Reference Type: RESULT

PMID: 31352621 (View on PubMed)

Cozzolino M, Diaz-Gimeno P, Pellicer A, Garrido N. Evaluation of the endometrial receptivity assay and the preimplantation genetic test for aneuploidy in overcoming recurrent implantation failure. J Assist Reprod Genet. 2020 Dec;37(12):2989-2997. doi: 10.1007/s10815-020-01948-7. Epub 2020 Sep 24.

Reference Type: RESULT

PMID: 32974805 (View on PubMed)

Simon C, Gomez C, Cabanillas S, Vladimirov I, Castillon G, Giles J, Boynukalin K, Findikli N, Bahceci M, Ortega I, Vidal C, Funabiki M, Izquierdo A, Lopez L, Portela S, Frantz N, Kulmann M, Taguchi S, Labarta E, Colucci F, Mackens S, Santamaria X, Munoz E, Barrera S, Garcia-Velasco JA, Fernandez M, Ferrando M, Ruiz M, Mol BW, Valbuena D; ERA-RCT Study Consortium Group. A 5-year multicentre randomized controlled trial comparing personalized, frozen and fresh blastocyst transfer in IVF. Reprod Biomed Online. 2020 Sep;41(3):402-415. doi: 10.1016/j.rbmo.2020.06.002. Epub 2020 Jun 15.

Reference Type: RESULT

PMID: 32723696 (View on PubMed)

Bergin K, Eliner Y, Duvall DW Jr, Roger S, Elguero S, Penzias AS, Sakkas D, Vaughan DA. The use of propensity score matching to assess the benefit of the endometrial receptivity analysis in frozen embryo transfers. Fertil Steril. 2021 Aug;116(2):396-403. doi: 10.1016/j.fertnstert.2021.03.031. Epub 2021 Apr 27.

Reference Type: RESULT

PMID: 33926718 (View on PubMed)

Riestenberg C, Kroener L, Quinn M, Ching K, Ambartsumyan G. Routine endometrial receptivity array in first embryo transfer cycles does not improve live birth rate. Fertil Steril. 2021 Apr;115(4):1001-1006. doi: 10.1016/j.fertnstert.2020.09.140. Epub 2021 Jan 15.

Reference Type: RESULT

PMID: 33461752 (View on PubMed)

Rienzi L, Gracia C, Maggiulli R, LaBarbera AR, Kaser DJ, Ubaldi FM, Vanderpoel S, Racowsky C. Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance. Hum Reprod Update. 2017 Mar 1;23(2):139-155. doi: 10.1093/humupd/dmw038.

Reference Type: RESULT

PMID: 27827818 (View on PubMed)

Roque M, Haahr T, Geber S, Esteves SC, Humaidan P. Fresh versus elective frozen embryo transfer in IVF/ICSI cycles: a systematic review and meta-analysis of reproductive outcomes. Hum Reprod Update. 2019 Jan 1;25(1):2-14. doi: 10.1093/humupd/dmy033.

Reference Type: RESULT

PMID: 30388233 (View on PubMed)

Zaat T, Zagers M, Mol F, Goddijn M, van Wely M, Mastenbroek S. Fresh versus frozen embryo transfers in assisted reproduction. Cochrane Database Syst Rev. 2021 Feb 4;2(2):CD011184. doi: 10.1002/14651858.CD011184.pub3.

Reference Type: RESULT

PMID: 33539543 (View on PubMed)

Mackens S, Santos-Ribeiro S, van de Vijver A, Racca A, Van Landuyt L, Tournaye H, Blockeel C. Frozen embryo transfer: a review on the optimal endometrial preparation and timing. Hum Reprod. 2017 Nov 1;32(11):2234-2242. doi: 10.1093/humrep/dex285.

Reference Type: RESULT

PMID: 29025055 (View on PubMed)

Shaodi Z, Qiuyuan L, Yisha Y, Cuilian Z. The effect of endometrial thickness on pregnancy outcomes of frozen-thawed embryo transfer cycles which underwent hormone replacement therapy. PLoS One. 2020 Sep 24;15(9):e0239120. doi: 10.1371/journal.pone.0239120. eCollection 2020.

Reference Type: RESULT

PMID: 32970718 (View on PubMed)

Groenewoud ER, Cantineau AE, Kollen BJ, Macklon NS, Cohlen BJ. What is the optimal means of preparing the endometrium in frozen-thawed embryo transfer cycles? A systematic review and meta-analysis. Hum Reprod Update. 2013 Sep-Oct;19(5):458-70. doi: 10.1093/humupd/dmt030. Epub 2013 Jul 2.

Reference Type: RESULT

PMID: 23820515 (View on PubMed)

Jing S, Li XF, Zhang S, Gong F, Lu G, Lin G. Increased pregnancy complications following frozen-thawed embryo transfer during an artificial cycle. J Assist Reprod Genet. 2019 May;36(5):925-933. doi: 10.1007/s10815-019-01420-1. Epub 2019 Mar 29.

Reference Type: RESULT

PMID: 30924053 (View on PubMed)

Liu X, Shi W, Shi J. Natural cycle frozen-thawed embryo transfer in young women with regular menstrual cycles increases the live-birth rates compared with hormone replacement treatment: a retrospective cohort study. Fertil Steril. 2020 Apr;113(4):811-817. doi: 10.1016/j.fertnstert.2019.11.023. Epub 2020 Mar 5.

Reference Type: RESULT

PMID: 32147171 (View on PubMed)

Melnick AP, Setton R, Stone LD, Pereira N, Xu K, Rosenwaks Z, Spandorfer SD. Replacing single frozen-thawed euploid embryos in a natural cycle in ovulatory women may increase live birth rates compared to medicated cycles in anovulatory women. J Assist Reprod Genet. 2017 Oct;34(10):1325-1331. doi: 10.1007/s10815-017-0983-6. Epub 2017 Jun 24.

Reference Type: RESULT

PMID: 28647784 (View on PubMed)

Wang A, Murugappan G, Kort J, Westphal L. Hormone replacement versus natural frozen embryo transfer for euploid embryos. Arch Gynecol Obstet. 2019 Oct;300(4):1053-1060. doi: 10.1007/s00404-019-05251-4. Epub 2019 Jul 23.

Reference Type: RESULT

PMID: 31338657 (View on PubMed)

Del Carmen Nogales M, Cruz M, de Frutos S, Martinez EM, Gaytan M, Ariza M, Bronet F, Garcia-Velasco JA. Association between clinical and IVF laboratory parameters and miscarriage after single euploid embryo transfers. Reprod Biol Endocrinol. 2021 Dec 14;19(1):186. doi: 10.1186/s12958-021-00870-6.

Reference Type: RESULT

PMID: 34906128 (View on PubMed)

Zanatta A, Rocha AM, Carvalho FM, Pereira RM, Taylor HS, Motta EL, Baracat EC, Serafini PC. The role of the Hoxa10/HOXA10 gene in the etiology of endometriosis and its related infertility: a review. J Assist Reprod Genet. 2010 Dec;27(12):701-10. doi: 10.1007/s10815-010-9471-y. Epub 2010 Sep 7.

Reference Type: RESULT

PMID: 20821045 (View on PubMed)

Merviel P, Challier JC, Carbillon L, Foidart JM, Uzan S. The role of integrins in human embryo implantation. Fetal Diagn Ther. 2001 Nov-Dec;16(6):364-71. doi: 10.1159/000053942.

Reference Type: RESULT

PMID: 11694740 (View on PubMed)

Wang W, Sung N, Gilman-Sachs A, Kwak-Kim J. T Helper (Th) Cell Profiles in Pregnancy and Recurrent Pregnancy Losses: Th1/Th2/Th9/Th17/Th22/Tfh Cells. Front Immunol. 2020 Aug 18;11:2025. doi: 10.3389/fimmu.2020.02025. eCollection 2020.

Reference Type: RESULT

PMID: 32973809 (View on PubMed)

Donoghue JF, Paiva P, Teh WT, Cann LM, Nowell C, Rees H, Bittinger S, Obers V, Bulmer JN, Stern C, McBain J, Rogers PAW. Endometrial uNK cell counts do not predict successful implantation in an IVF population. Hum Reprod. 2019 Dec 1;34(12):2456-2466. doi: 10.1093/humrep/dez194.

Reference Type: RESULT

PMID: 31825483 (View on PubMed)

Other Identifiers

KSVGH22-CT7-07

Identifier Type: -

Identifier Source: org_study_id