Ovarian Reserve and Semen Parameters Evolution During Adjuvant Therapy in Melanoma

NCT ID: NCT05429138

Last Updated: 2025-11-18

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

80 participants

Study Classification

OBSERVATIONAL

Study Start Date

2022-12-07

Study Completion Date

2026-12-31

Brief Summary

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Prospective multicentric study including women aged 18 to 37 and men aged 18 to 45 during their visit to centers for the study and storage of human sperm and eggs (CECOS). Subjects will be included before adjuvant treatment initiation (T0) and immediately after treatment (approximately 1 year after initiation, T1), and, in late post treatment (1 year after treatment cessation, T2).

Expected results: This study will evaluate the evolution of AMH, AFC, and semen parameters in our cohort of melanoma patients treated with anti-PD-1 and targeted therapy in an adjuvant and neoadjuvantsettings.

Detailed Description

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To the best of our knowledge, no data is available in humans on the impact of anti-PD-1 immunotherapies and therapies targeting the MAP kinase pathway, in adjuvant settings, on ovarian reserve and semen quality.

Main objective: to measure pre-treatment (T0) and immediate post-treatment (T1) evolution of anti-Müllerian hormone (AMH) levels reflecting the ovarian reserve in women, and of the total motile sperm count per ejaculate in men, in patients of childbearing age treated with anti-PD-1 immunotherapy or targeted therapies in an adjuvant situation for melanoma at high risk of recurrence.

Method: Prospective multicentric study including women aged 18 to 37 and men aged 18 to 45 during their visit to centers for the study and storage of human sperm and eggs (CECOS). Subjects will be included before adjuvant treatment initiation (T0) and immediately after treatment (approximately 1 year after initiation, T1), and, in late post treatment (1 year after treatment cessation, T2).

At each of their visit to CECOS (T0, T1 and T2), the women will have an AMH assay and an antral follicle count (AFC) by ultrasound while the men will perform a semen analysis (count, spermocytogram and staining with aniline blue for analysis of chromatin condensation).

A standardized questionnaire aimed at collecting data about factors that may alter fertility will be submitted at each of these visits.

Expected results: This study will evaluate the evolution of AMH, AFC, and semen parameters in our cohort of melanoma patients treated with anti-PD-1 and targeted therapy in an adjuvant settings.

Conditions

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Melanoma

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Immunotherapy cohort

Patient that received adjuvant immunotherapy

biological sampling

Intervention Type OTHER

Biological sampling: blood and semen

Targeted therapy

Patient that received adjuvant targeted therapy

biological sampling

Intervention Type OTHER

Biological sampling: blood and semen

Interventions

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biological sampling

Biological sampling: blood and semen

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Patients must have provided a signed, dated and written consent prior to any specific procedures, sampling and analyses
* Patients with valide Health Inssurance Scheme
* Female between 18 and 37 years old and male between 18 and 45 years old
* During the 2 months before the introduction of an approved regimen of adjuvant anti-PD-1 immunotherapy or neoadjuvant plus adjuvant or targeted therapy for an high-risk of reccurence melanoma
* Adjuvant or neoadjuvant plus adjuvant treatment must be prescribed as part of routine care

Exclusion Criteria

* Individuals deprived of liberty or placed under the authority of a tutor
* Patients unable to understand, read and/or sign an informed consent
* History of cytotoxic treatment before T0 that can alterate the studied parameters
* In male, totale motile sperm count per ejaculate inferior to 39 millions at T0
* In women, an age-specific AMH level inferior to the 10th percentile at T0
* Any condition which in the Investigator's opinion would jeopardize compliance with the protocol of the study
* Patients that will received an investigational treatment during the study timeframe (an observational research is allowed)
* Patients who have changed the type of adjuvant treatment during adjuvant treatment (targeted switch therapy versus targeted immunotherapy and vice versa) or after the neoadjuvant phase due to the pathological response obtained.
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Assistance Publique Hopitaux De Marseille

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Nausicaa Malissen, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

AP-HM

Locations

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CHU de Bordeaux

Bordeaux, , France

Site Status RECRUITING

AP-HM

Marseille, , France

Site Status RECRUITING

Hôpital Saint-Joseph

Marseille, , France

Site Status RECRUITING

CHU Montpellier

Montpellier, , France

Site Status RECRUITING

CHU de Nantes

Nantes, , France

Site Status RECRUITING

CHU NICE L'archet

Nice, , France

Site Status RECRUITING

CHRU de Nîmes

Nîmes, , France

Site Status RECRUITING

APHP- Ambroise Paré

Paris, , France

Site Status RECRUITING

APHP-Cochin

Paris, , France

Site Status RECRUITING

APHP-Saint Louis

Paris, , France

Site Status RECRUITING

Oncôpole de Toulouse

Toulouse, , France

Site Status RECRUITING

Institut Gustave Roussy

Villejuif, , France

Site Status RECRUITING

Countries

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France

Central Contacts

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Nausicaa Malissen, MD, PhD

Role: CONTACT

0491384215

DRSMR AP-HM

Role: CONTACT

Facility Contacts

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Nausicaa MALISSEN

Role: primary

Vanessa Loup

Role: primary

Henri MONTAUDIE

Role: primary

Other Identifiers

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2021-A02601-40

Identifier Type: -

Identifier Source: org_study_id

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