Efficacy of Daromun Neoadjuvant Intratumoral Treatment in Clinical Stage IIIB/C/D Melanoma Patients

NCT ID: NCT03567889

Last Updated: 2025-12-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE3

Total Enrollment

186 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-09-20

Study Completion Date

2031-12-31

Brief Summary

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The trial aims to evaluate the efficacy of Daromun neoadjuvant treatment followed by surgery and adjuvant therapy to improve in a statistically significant manner the recurrence-free survival (RFS) of Stage IIIB/C/D melanoma patients with respect to the standard of care (surgery and adjuvant therapy).

Detailed Description

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The present study is an open-label, randomized, controlled, two-arm multi-center study of the efficacy of Daromun neoadjuvant intratumoral treatment followed by surgery and adjuvant therapy versus surgery and adjuvant therapy in clinical stage III B/C/D melanoma patients. 186 patients will be randomized in a 1:1 ratio to receive Daromun treatment followed by surgery and adjuvant therapy (Arm 1) or surgery and adjuvant therapy (Arm 2).

In both arms, follow-up for assessing recurrence-free survival will be performed up to five years after randomization. Survival information will also be collected in the following year (up to six years in total after randomization).

This is an open-label study, so there is no blinding.

Patients who successfully complete the screening evaluations and are eligible for participation in the study will be enrolled and randomly assigned (1:1) to two parallel treatment arms: Daromun plus surgery and adjuvant therapy (Arm 1) or surgery and adjuvant therapy (Arm 2).

To ensure a balance across treatment groups, stratified randomization with permuted block will be used and separate randomization list for each subgroup (stratum) will be produced. Patients will be stratified on the basis of the following prognostic factors:

* Stage of disease (3 levels): Stage IIIB vs. Stage IIIC vs Stage IIID
* Planned post-surgical adjuvant therapy (2 levels): anti-PD-1 and other adjuvant therapies.

The primary objective of the study is to demonstrate that a neoadjuvant Daromun treatment followed by surgery and adjuvant therapy improves in a statistically significant manner the recurrence-free survival (RFS) of Stage IIIB/C/D melanoma patients with respect to the standard of care (surgery and adjuvant therapy).

Primary endpoint of the study is RFS in a time-to-event analysis in the Daromun plus surgery and adjuvant therapy treatment group (Arm 1) versus the surgery plus adjuvant therapy control group (Arm 2). Analysis will be based on the "Intention To Treat" population.

The key secondary objective of the study is to demonstrate that a neoadjuvant Daromun treatment followed by surgery and adjuvant therapy improves in a statistically significant manner the overall survival (OS) of patients with resectable Stage IIIB/C/D melanoma patients with respect to the standard of care (surgery and adjuvant therapy).

For patients enrolled in both arms, local approved post-surgery adjuvant therapies (as part of the standard of care) are allowed and decided at the investigator's discretion. These include high-dose interferon- α2b, anti-CTLA-4 antibodies (e.g. Ipilimumab), anti-PD1 antibodies (e.g. Nivolumab, Pembrolizumab), targeted therapies (e.g. Dabrafenib + Trametinib), or other new local approved treatments.

Conditions

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Melanoma Stage IIIB Melanoma Stage IIIC Melanoma Stage IIID

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Total patients: approximately 186. Daromun plus surgery and adjuvant therapy treatment group (Arm 1): 93 evaluable patients. Surgery and adjuvant therapy (Arm 2): 93 evaluable patients. Patients enrolled will be randomized to the two different arms of the study.
Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Daromun plus Surgery and Adjuvant therapy

Arm-1 patients will follow these steps:

1. screening period,
2. 4-week open-label treatment period,
3. surgery within a maximum of 4 weeks,
4. adjuvant therapy.

Group Type EXPERIMENTAL

Daromun

Intervention Type DRUG

Patients will receive intratumoral administrations into injectable cutaneous, subcutaneous, and nodal tumors of Daromun once weekly for up to 4 weeks.

Surgery

Intervention Type PROCEDURE

Patients will receive surgery.

Adjuvant therapy

Intervention Type DRUG

Patients will receive adjuvant therapy at the investigator's discretion following the surgery.

Surgery and adjuvant therapy

Arm-2 patients will follow these steps:

1. Screening period,
2. direct surgery within 4 weeks from randomization,
3. adjuvant therapy.

Group Type ACTIVE_COMPARATOR

Surgery

Intervention Type PROCEDURE

Patients will receive surgery.

Adjuvant therapy

Intervention Type DRUG

Patients will receive adjuvant therapy at the investigator's discretion following the surgery.

Interventions

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Daromun

Patients will receive intratumoral administrations into injectable cutaneous, subcutaneous, and nodal tumors of Daromun once weekly for up to 4 weeks.

Intervention Type DRUG

Surgery

Patients will receive surgery.

Intervention Type PROCEDURE

Adjuvant therapy

Patients will receive adjuvant therapy at the investigator's discretion following the surgery.

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

1. Histologically or cytologically confirmed diagnosis of clinical stage IIIB, IIIC, and IIID (AJCC 8th edition) locoregional melanoma that is eligible for complete surgical resection of all metastases (surgically resectable).
2. Eligible subjects must have measurable disease and must be candidate for intralesional therapy with at least one injectable cutaneous, subcutaneous, or nodal melanoma lesion (≥ 10 mm in longest diameter) or with multiple injectable lesions that in aggregate have a longest diameter of ≥ 10 mm.
3. Prior anti-tumor treatment for the primary melanoma lesion, including surgery and approved adjuvant treatments (e.g., radiotherapy, immune checkpoint inhibitors, BRAF/MEK inhibitors, etc.) is allowed. Before enrollment in the study, a wash-out period of 6 weeks is required and toxicities from prior treatments should be resumed to Grade ≤1.
4. Males or females, age ≥ 18 years.
5. ECOG Performance Status/WHO Performance Status ≤ 1.
6. Life expectancy of \> 24 months.
7. Absolute neutrophil count \> 1.5 x 109/L.
8. Hemoglobin \> 9.0 g/dL.
9. Platelets \> 100 x 109/L.
10. Total bilirubin ≤ 30 μmol/L (or ≤ 2.0 mg/dl).
11. ALT and AST ≤ 2.5 x the upper limit of normal (ULN).
12. Serum creatinine \< 1.5 x ULN.
13. LDH serum level ≤ 1.5 x ULN.
14. Documented negative test for HIV, HBV and HCV. For HBV serology, the determination of HBsAg and anti-HBcAg Ab is required. In patients with serology documenting previous exposure to HBV (i.e. positive anti-HBsAg with not vaccination and/or positive anti-HBcAg Ab), negative serum HBV-DNA is also required.
15. All acute toxic effects (excluding alopecia) of any prior therapy must have resolved to National Cancer Institute (NCI) Common Terminology Criteria for Adverse Events (CTCAE) (v4.03) Grade ≤ 1 unless otherwise specified above.
16. All women of childbearing potential (WOCBP) must have negative pregnancy test results at the screening. WOCBP must be using, from the screening to three months following the last study drug administration, highly effective contraception methods. WOCBP and effective contraception methods are defined by the "Recommendations for contraception and pregnancy testing in clinical trials" issued by the Head of Medicine Agencies' Clinical Trial Facilitation Group and which include, for instance, progesterone-only or combined (estrogen- and progesterone-containing) hormonal contraception associated with inhibition of ovulation, intrauterine devices, intrauterine hormone-releasing systems, bilateral tubal occlusion, vasectomized partner or sexual abstinence. Pregnancy test will be repeated at the safety visit (only WOCBP and only for patients in Arm 1).
17. Male patients with WOCBP partners must agree to use simultaneously two acceptable methods of contraception (i.e. spermicidal gel plus condom) from the screening to three months following the last study drug administration.
18. Evidence of a personally signed and dated informed consent document indicating that the subject has been informed of all pertinent aspects of the study.
19. Willingness and ability to comply with the scheduled visits, treatment plan, laboratory tests and other study procedures.

Exclusion Criteria

1. Uveal melanoma or mucosal melanoma
2. Evidence of distant metastases at screening.
3. Previous or concurrent cancer that is distinct in primary site or histology from the cancer being evaluated in this study except: cervical carcinoma in situ, curatively treated basal cell carcinoma, superficial bladder tumors (Ta, Tis \& T1), second primary melanoma in situ or any cancer curatively treated ≥ 5 years prior to study entry.
4. Presence of active infections (e.g. requiring antimicrobial therapy) or other severe concurrent disease, which, in the opinion of the investigator, would place the patient at undue risk or interfere with the study.
5. History within the last year of acute or subacute coronary syndromes including myocardial infarction, unstable or severe stable angina pectoris.
6. Inadequately controlled cardiac arrhythmias including atrial fibrillation.
7. Heart insufficiency (\> Grade II, New York Heart Association (NYHA) criteria).
8. LVEF ≤ 50% and/or abnormalities observed during baseline ECG and Echocardiogram investigations that are considered as clinically significant by the investigator.
9. Uncontrolled hypertension.
10. Ischemic peripheral vascular disease (Grade IIb-IV).
11. Severe diabetic retinopathy.
12. Active autoimmune disease.
13. History of organ allograft or stem cell transplantation.
14. Recovery from major trauma including surgery within 4 weeks prior to enrollment.
15. Known history of allergy to IL2, TNF, or other human proteins/peptides/antibodies or any other constituent of the product.
16. Breast feeding female.
17. Anti-tumor therapy (except small surgery) within 4 weeks before enrollment.
18. Previous in vivo exposure to monoclonal antibodies for biological therapy in the 6 weeks before enrollment.
19. Planned administration of growth factors or immunomodulatory agents within 7 days before enrollment.
21. Any conditions that in the opinion of the investigator could hamper compliance with the study protocol.
22. Previous enrolment and randomization in the same study.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Philogen S.p.A.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Mayo Clinic Hospital

Phoenix, Arizona, United States

Site Status RECRUITING

UC San Diego Moores Cancer Center

La Jolla, California, United States

Site Status ACTIVE_NOT_RECRUITING

UC Irvine Health-Chao Family Comprehensive Cancer Center

Orange, California, United States

Site Status ACTIVE_NOT_RECRUITING

Moffitt Cancer Center

Tampa, Florida, United States

Site Status RECRUITING

Winship Cancer Institute, Emory university

Atlanta, Georgia, United States

Site Status RECRUITING

Rush University Medical Center

Chicago, Illinois, United States

Site Status ACTIVE_NOT_RECRUITING

University of Iowa Hospitals and Clinics

Iowa City, Iowa, United States

Site Status ACTIVE_NOT_RECRUITING

Mayo Clinic

Rochester, Minnesota, United States

Site Status RECRUITING

Rutgers Cancer Institute, 195 Little Albany Street

New Brunswick, New Jersey, United States

Site Status RECRUITING

Ambulatory Care Center at NYC Langarone Health

New York, New York, United States

Site Status ACTIVE_NOT_RECRUITING

Memorial Sloan Kettering Cancer Center - Main Campus

Ney York, New York, United States

Site Status RECRUITING

Duke University Medical Center - Duke Cancer Center

Durham, North Carolina, United States

Site Status RECRUITING

Ohio State University Wexner Medical Center

Columbus, Ohio, United States

Site Status RECRUITING

St. Luke's Cancer Center, Clinical Trial, 3rd floor, 1600 St. Luke's Blvd.

Easton, Pennsylvania, United States

Site Status ACTIVE_NOT_RECRUITING

Penn State Cancer Institute

Hershey, Pennsylvania, United States

Site Status RECRUITING

Fox Chase Cancer Center 333 Cottman Avenue

Philadelphia, Pennsylvania, United States

Site Status RECRUITING

The University of Texas M.D. Anderson Cancer Center

Houston, Texas, United States

Site Status RECRUITING

Huntsman Cancer Institute, University of Utah 2000 Circle of Hope

Salt Lake City, UT, Utah, United States

Site Status RECRUITING

VCU - McGlothlin Medical Education Center

Richmond, Virginia, United States

Site Status RECRUITING

Hospital Universitari Germans Trias i Pujol

Badalona, Barcelona, Spain

Site Status RECRUITING

Hospital de la Santa Creu i Sant Pau

Barcelona, Barcelona, Spain

Site Status RECRUITING

Fir Huvh Fundacio Institut De Recerca Hospital Universitari Vall De Hebron

Barcelona, Barcelona, Spain

Site Status RECRUITING

Hospital Clinic Barcelona

Barcelona, Barcelona, Spain

Site Status RECRUITING

El Hospital Universitario De Gran Canaria Dr. Negrin

Las Palmas de Gran Canaria, Canarie, Spain

Site Status RECRUITING

Fundacion Onkologikoa Fundazioa

Donostia / San Sebastian, Gipuzkoa, Spain

Site Status RECRUITING

MD Anderson Cancer Center

Madrid, Madrid, Spain

Site Status ACTIVE_NOT_RECRUITING

Hospital Universitario 12 de Octubre

Madrid, Madrid, Spain

Site Status RECRUITING

Hospital Universitario Regional de Málaga

Málaga, Malaga, Spain

Site Status RECRUITING

Hospital Clínico Universitario Virgen de la Arrixaca

Murcia, Murcia, Spain

Site Status RECRUITING

Hospital Universitario Virgen De La Macarena

Seville, Sevilla, Spain

Site Status ACTIVE_NOT_RECRUITING

Hospital General Universitario de Valencia

Valencia, , Spain

Site Status RECRUITING

Universitätsspital Basel

Basel, Basel, Switzerland

Site Status ACTIVE_NOT_RECRUITING

Istituto Oncologico della Svizzera Italiana

Bellinzona, Bellinzona, Switzerland

Site Status RECRUITING

Insel Gruppe AG

Bern, Canton of Bern, Switzerland

Site Status ACTIVE_NOT_RECRUITING

Hôpitaux Universitaires de Genève

Geneva, Canton of Geneva, Switzerland

Site Status ACTIVE_NOT_RECRUITING

Kantonsspital St.Gallen

Sankt Gallen, Canton of St. Gallen, Switzerland

Site Status RECRUITING

Universitätsspital Zürich (USZ)

Zurich, Canton of Zurich, Switzerland

Site Status RECRUITING

Countries

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United States Spain Switzerland

Central Contacts

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Sheila Dakhel, PhD

Role: CONTACT

+41 (0) 43 544 88 02

Concetta Aulicino

Role: CONTACT

+39 0577 17 816

Facility Contacts

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Mahesh Seetharam, MD

Role: primary

Jonathan Zager

Role: primary

Michael Lowe

Role: primary

Anastasios Dimou, MD

Role: primary

Adam Berger

Role: primary

Danielle Bello

Role: primary

Georgia Beasley

Role: primary

Claire Verschraegen

Role: primary

Joseph Drabick

Role: primary

Jeffrey Farma

Role: primary

Hussein Tawbi

Role: primary

John Hyngstrom

Role: primary

Andrew Poklepovic

Role: primary

José Luis Manzano Mozo

Role: primary

+34615315823

Margarita Majem Tarruella

Role: primary

+34935565638

Eva Muñoz Couselo

Role: primary

+34932746085

Josep Malvehy Guilera

Role: primary

+34932275400

Elena Castro Gonzalez

Role: primary

+34928450658

Karmele Mujika Eizmendi

Role: primary

+34943328315

Pedro Luis Ortiz Romero

Role: primary

+34913908000

Elisabeth Pérez Ruiz

Role: primary

+34951308130

Pablo Cerezuela Fuentes

Role: primary

+34968369387

Alfonso Berrocal Jaime

Role: primary

+34963131800

Cristina Mangas

Role: primary

Nikolaus Wagner

Role: primary

Egle Ramelyte

Role: primary

References

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Gorry C, McCullagh L, O'Donnell H, Barrett S, Schmitz S, Barry M, Curtin K, Beausang E, Barry R, Coyne I. Neoadjuvant treatment for stage III and IV cutaneous melanoma. Cochrane Database Syst Rev. 2023 Jan 17;1(1):CD012974. doi: 10.1002/14651858.CD012974.pub2.

Reference Type DERIVED
PMID: 36648215 (View on PubMed)

Miura JT, Zager JS. Neo-DREAM study investigating Daromun for the treatment of clinical stage IIIB/C melanoma. Future Oncol. 2019 Nov;15(32):3665-3674. doi: 10.2217/fon-2019-0433. Epub 2019 Sep 20.

Reference Type DERIVED
PMID: 31538818 (View on PubMed)

Other Identifiers

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2023-507119-36-00

Identifier Type: CTIS

Identifier Source: secondary_id

PH-L19IL2TNF-01/18

Identifier Type: -

Identifier Source: org_study_id