Efficacy of VTP-300 in Chronic Hepatitis B Infection

NCT ID: NCT05343481

Last Updated: 2025-09-16

Basic Information

• Recruitment Status: ACTIVE_NOT_RECRUITING
• Clinical Phase: PHASE2
• Total Enrollment: 120 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2022-09-21
• Study Completion Date: 2026-10-31

Brief Summary

This is an open-label study to determine the efficacy, safety, tolerability and immunogenicity of ChAdOx1-HBV and MVA-HBV, together VTP-300, in combination with low-dose nivolumab, in patients with chronic HBV who are virally suppressed with oral anti-viral therapies.

Detailed Description

This is a multi-centre study conducted in 120 participants. All treatment groups will receive ChAdOx1-HBV on Day 1 and MVA-HBV on Day 29, and MVA boosts and nivolumab infusions as per treatment group as follows: Group 1: ChAdOx1-HBV, MVA-HBV and low dose nivolumab. Group 2: ChAdOx1-HBV, MVA HBV + low dose nivolumab, MVA HBV and low dose nivolumab. Group 3: ChAdOx1-HBV, MVA HBV, low dose nivolumab , MVA HBV. All participants return 7 days after each treatment (both immunotherapeutic and nivolumab infusion) visit to have chemistry and haematology safety laboratory studies obtained.

Participants are randomised to treatment in a 1:1:1 allocation. The primary objective of the study is to assess the efficacy of VTP-300 in combination with low-dose nivolumab in well-controlled CHB infection. The secondary objective of the study is to determine the safety and reactogenicity of the treatment regimens; this will be assessed by analysis of the incidence and severity of (serious) adverse events and any changes in laboratory values and vital signs. To assess the effect of VTP-300 in combination with low-dose nivolumab on the clearance of HBsAg and the impact of multiple booster doses of MVA-HBV and assess the appropriate timing of the use of low-dose nivolumab when used in combination with VTP-300.

Participants remain in the study for 12 months and attend clinic visits for treatment and assessments on Days 1, 8, 29, 36, 57, 85, 92, 113, 169, 252 and 336 (per Group allocation).

Conditions

Chronic Hepatitis B

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: SEQUENTIAL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Experimental: Group 1 ChAdOx1-HBV, MVA-HBV and nivolumab

Day 1: ChAdOx1-HBV 1 x 2.5 10\^10 vp IM injection Day 29: MVA-HBV 1 x 10\^8 pfu IM injection + nivolumab 0.3 mg/kg IV infusion

Group Type: EXPERIMENTAL

ChAdOx1-HBV

Intervention Type: BIOLOGICAL

Chimpanzee Adenovirus Oxford 1-vectored Hepatitis B virus immunotherapeutic

MVA-HBV

Intervention Type: BIOLOGICAL

Modified Vaccinia Ankara-vectored Hepatitis B virus immunotherapeutic

Nivolumab

Intervention Type: BIOLOGICAL

Human immunoglobulin G4 monoclonal antibody

Experimental: Group 2 ChAdOx1-HBV, MVA-HBV and nivolumab, MVA-HBV and nivolumab

Day 1: ChAdOx1-HBV 1 x 2.5 10\^10 vp IM injection Day 29: MVA-HBV 1 x 10\^8 pfu IM injection + nivolumab 0.3 mg/kg IV infusion Day 85: MVA-HBV 1 x 10\^8 pfu IM injection + nivolumab 0.3 mg/kg IV infusion

Group Type: EXPERIMENTAL

ChAdOx1-HBV

Intervention Type: BIOLOGICAL

Chimpanzee Adenovirus Oxford 1-vectored Hepatitis B virus immunotherapeutic

MVA-HBV

Intervention Type: BIOLOGICAL

Modified Vaccinia Ankara-vectored Hepatitis B virus immunotherapeutic

Nivolumab

Intervention Type: BIOLOGICAL

Human immunoglobulin G4 monoclonal antibody

Experimental: Group 3 ChAdOx1-HBV, MVA-HBV, nivolumab, MVA-HBV

Day 1: ChAdOx1-HBV 1 x 2.5 10\^10 vp IM injection Day 29: MVA-HBV 1 x 10\^8 pfu IM injection Day 36: Nivolumab 0.3 mg/kg IV infusion Day 85: MVA-HBV 1 x 10\^8 pfu IM injection

Group Type: EXPERIMENTAL

ChAdOx1-HBV

Intervention Type: BIOLOGICAL

Chimpanzee Adenovirus Oxford 1-vectored Hepatitis B virus immunotherapeutic

MVA-HBV

Intervention Type: BIOLOGICAL

Modified Vaccinia Ankara-vectored Hepatitis B virus immunotherapeutic

Nivolumab

Intervention Type: BIOLOGICAL

Human immunoglobulin G4 monoclonal antibody

Interventions

ChAdOx1-HBV

Chimpanzee Adenovirus Oxford 1-vectored Hepatitis B virus immunotherapeutic

Intervention Type: BIOLOGICAL

MVA-HBV

Modified Vaccinia Ankara-vectored Hepatitis B virus immunotherapeutic

Intervention Type: BIOLOGICAL

Nivolumab

Human immunoglobulin G4 monoclonal antibody

Intervention Type: BIOLOGICAL

Other Intervention Names

Opdivo 10mg/ml concentrate for solution for infusion

Eligibility Criteria

Inclusion Criteria

1. Adult males or females aged ≥18 to ≤65 years at screening (according to country/local regulations)

2. BMI ≤35 kg/m2

3. Able to provide informed consent indicating they understand the purpose of, and procedures required, for the study and are willing to participate

4. If female, willing not to become pregnant up to 8 weeks after the last dose of study vaccine and up to 5 months after the last dose of nivolumab

5. If female: Not pregnant or breast feeding and one of the following:

• Of non-childbearing potential (i.e., women who have had a hysterectomy or tubal ligation or are postmenopausal, as defined by no menses in ≥1 year and without an alternative medical cause)
• Of childbearing potential but agrees to practice highly effective contraception for 4 weeks prior to study vaccine and 8 weeks after VTP-300 and 5 months after the last dose of nivolumab. Highly effective methods of contraception include one or more of the following:

• Male partner who is sterile (medically effective vasectomy) prior to the female participant's entry into the study and is the sole sexual partner for the female participant
• Combined (oestrogen and progestogen-containing) hormonal contraception associated with inhibition of ovulation: oral, intravaginal or transdermal
• Progestogen-only hormonal contraception associated with inhibition of ovulation: oral, injectable or implantable
• An intrauterine device
• Bilateral tubal occlusion
• Abstinence from heterosexual intercourse as their preferred and usual lifestyle (abstinent on a long term and persistent basis) and agree to remain abstinent

6. Documented evidence of CHB infection (e.g., HBsAg positive ≥6 months with detectable HBsAg levels at screening; both HBeAg+ and HBeAg- allowed)

7. Receipt of only either entecavir, tenofovir (tenofovir alafenamide fumarate or tenofovir disoproxil fumarate) or besifovir for at least 6 months before screening

8. HBV-DNA viral load ≤ 1,000 IU/mL

9. HBsAg levels > 10 and ≤ 4,000 IU/mL

Exclusion Criteria

1. Presence of any significant acute or chronic, uncontrolled medical or psychiatric illness in the opinion of the investigator would affect the safety of the participant or the evaluation of the data or interfere with adherence to the study requirements

2. Medical history that is thought to increase the participant's risk of reaction to a vaccine, including but not limited to capillary leak syndrome; transverse myelitis, Guillain Barré syndrome, thrombosis with thrombocytopenia syndrome (also termed vaccine-induced thrombotic thrombocytopenia); heparin-induced thrombocytopenia HCV RNA positive

3. HIV antibody positive and active hepatitis C (antibody positive and then DNA positive)

4. Co-infection with hepatitis D virus (HDV)

5. Documented cirrhosis or advanced fibrosis indicated by a liver biopsy within 6 months prior to Day 0 (Metavir activity grade A4 and stage F4; Ishak stages 5 - 6).

6. In the absence of a documented liver biopsy, either 1 of the following (not both):

• Screening Fibroscan with a result >9 kilopascals (kPa) (or the equivalent) within ≤ 6 months of screening, OR
• Both screening FibroTest >0.48 and aspartate aminotransferase (AST) to platelet ratio index (APRI) of >1.

7. ALT >3 x ULN, or INR >1.5 unless the participant was stable on an anticoagulant regimen affecting INR, albumin <3.2 g/dL, direct bilirubin >1.5 x ULN, platelet count <100,000/µL.

8. A history of liver decompensation (e.g., ascites, encephalopathy or variceal haemorrhage)

9. Prior hepatocellular carcinoma

10. Chronic liver disease of a non-HBV aetiology. (Note that Gilbert's syndrome, asymptomatic gallstones and non-alcoholic fatty liver not associated with steatohepatitis are not exclusions)

11. History or evidence of autoimmune disease or known immunodeficiency of any cause except history of autoimmune thyroiditis if the participant is stable on replacement therapy

12. Evidence of interstitial lung disease, active pneumonitis, myocarditis or a history of myocarditis

13. Prolonged therapy with immunomodulators (e.g., corticosteroids such as prednisone >10 mg/day) or biologics (e.g., monoclonal antibodies, IFN) within 3 months of Day 1. Inhaled, intra-articular, intra-bursal or topical corticosteroids are allowed. Physiologic corticosteroid replacement therapy for adrenal or pituitary insufficiency is allowed.

14. Receipt of immunoglobulin or other blood products within 3 months prior to Day 1

15. Receipt of any investigational drug or vaccine within 3 months prior to Day 1

16. Receipt of any non-oral adenoviral-based vaccine within 3 months prior to administration of ChAdOx1-HBV on Day 1

17. Severe reaction to any vaccine, requiring medical attention

18. Receipt of any live vaccines within 30 days prior to Day 1

19. Receipt of any inactivated non-live vaccines (e.g., mRNA, inactivated vaccines, toxoid vaccines) within 14 days prior to Day 1

20. History of severe hypersensitivity or anaphylactic reactions likely to be exacerbated by any component of VTP-300 or nivolumab, including severe allergy to egg

21. Malignancy within 5 years prior to screening with the exception of basal cell or squamous epithelial carcinomas of the skin that have been resected with no evidence of metastatic disease for 3 years. Participants under evaluation for possible malignancy are not eligible

22. Current alcohol or substance abuse judged by the investigator to potentially interfere with participant safety or compliance

23. Any laboratory test which is abnormal, and which is deemed by the investigator to be clinically significant

24. Any other finding that, in the opinion of the investigator, deems the participant unsuitable for the study

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 65 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Barinthus Biotherapeutics

INDUSTRY

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Locations

Prince Of Wales Hospital

Hong Kong, , Hong Kong

Queen Mary Hospital

Hong Kong, , Hong Kong

Chia-Yi Christian Hospital

Chiayi City, , Taiwan

Dalin Tzu Chi Hospital

Chiayi City, , Taiwan

Kaohsiung Medical University Chung-Ho Memorial Hospital

Kaohsiung City, , Taiwan

Chang Gung Memorial Hospital Kaohsiung

Kaohsiung City, , Taiwan

China Medical University Hospital

Taichung, , Taiwan

National Cheng Kung University Hospital

Tainan City, , Taiwan

National Taiwan University Hospital

Taipei, , Taiwan

Linkou Chang Gung Memorial Hospital

Taoyuan, , Taiwan

Chulabhorn Hospital

Bangkok, , Thailand

King Chulalongkorn Memorial Hospital

Bangkok, , Thailand

HIV Netherlands-Australia-Thailand Research Collaboration

Bangkok, , Thailand

Hospital For Tropical Diseases

Bangkok, , Thailand

Maharaj Nakorn Chiang Mai Hospital

Chiang Mai, , Thailand

Research Institute For Health Sciences

Chiang Mai, , Thailand

Srinagarind Hospital

Khon Kaen, , Thailand

Bamrasnaradura Infectious Diseases Institute

Nonthaburi, , Thailand

Countries

Hong Kong; Taiwan; Thailand

Other Identifiers

HBV-003

Identifier Type: -

Identifier Source: org_study_id