Siderophores for Imaging Infection Using 68Ga-DFO

NCT ID: NCT05285072

Last Updated: 2022-03-17

Basic Information

• Recruitment Status: UNKNOWN
• Total Enrollment: 30 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2022-03-15
• Study Completion Date: 2024-01-31

Brief Summary

This is a single center pilot study involving a single study visit for participants, with remote follow up data collected at 24 hours. Follow up at 3 months and 6 months later occurs as standard of care.

The purpose of the study is to find out if a new type of imaging tracer (68Ga-DFO) can be used to show infection in patients with vascular grafts using PET/CT scans. These infections may be associated with significant ill health and mortality and can be difficult to diagnose. Effective treatments can require major surgery and long-term antibiotic therapy which may not be well tolerated nor feasible. Development of new imaging tracers that could detect bacteria causing graft infections with PET-CT scanners has great potential to benefit patients being considered for vascular surgery.

A PET-CT scan combines images from a CT (Computerised Tomography) scan and a PET (Positron Emission Tomography) scan. The CT scan takes a series of X-rays across the organs inside the body. The PET scan uses a mildly radioactive tracer to show up areas of activity inside the body. The 68Ga-DFO tracer mimics particles that bacteria make to take up iron from the body to help them grow. The investigators hope this new tracer will go to areas where bacteria are causing infection and tell if the graft is infected. The investigators hope this type of tracer could be a better way to show infection than the tests currently used to diagnose infection.

Detailed Description

This study is a first in human mechanistic study hypothesizing that siderophore complexes labelled with a radiotracer (Gallium-68) will be taken up in sites of infection in patients with a vascular graft.

Study objectives are to:

i) Determine if siderophore uptake occurs in sites of infection using the radiolabelled siderophore 68Ga-DFO in patients with a vascular graft on PET/CT scans.

ii) Determine if siderophore uptake as measured by the radiolabelled siderophore 68Ga-DFO is specific for infection by exploring whether uptake occurs in individuals with vascular grafts without infection and by characterising the uptake in normal tissues.

The injected substances are well-known licensed products.

The study will involve performing 68Ga-DFO PET/CT scans in 30 patients with vascular grafts, including 25 patients with diagnosed infection according to current syndromic criteria and 5 patients with a vascular graft but no evidence of any infection. Patients with infection will be recruited from patients who have had a prior FDG-PET-CT scan performed as part of their routine care which shows suspected graft infection.

All patients will have 'static' PET-CT scans following injection of the tracer. The scans will take 30 minutes.

The first ten patients with graft infection will also have 'dynamic' scans performed immediately after tracer injection lasting for 90 minutes. The result of these scans (e.g. time activity curves) will inform the scan protocol of the subsequent patients (e.g. uptake time). Up to 10 of these patients will have venous blood sampling at 8 time points up to 120 minutes after injection.

Patients will be followed up in person or by telephone 24 hours after the scan. Apart from this no specific follow up procedure outside standard of care is planned within this study.

Conditions

Vascular Graft Infection

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: PROSPECTIVE

Study Groups

Vascular graft infection

Patients with confirmed vascular graft infection according to current syndromic criteria.

PET-CT scan

Intervention Type: RADIATION

PET-CT scan using 68Ga-DFO

Vascular graft without infection

Patients with no clinical evidence of infection and CRP less than 5.

PET-CT scan

Intervention Type: RADIATION

PET-CT scan using 68Ga-DFO

Interventions

PET-CT scan

PET-CT scan using 68Ga-DFO

Intervention Type: RADIATION

Eligibility Criteria

Inclusion Criteria

• Age 18 and older
• Women of childbearing potential must have documented negative pregnancy test prior to tracer administration.
• Willing and able to give informed consent
• Able to comply with the PET imaging protocol
• Patients with vascular grafts in situ
• Positive FDG-PET/CT in the case of patients with suspected or diagnosed graft infections

Exclusion Criteria

• Aged under 18
• Pregnancy or lactation
• Prisoners
• Any lack of capacity to consent, including lack of adequate understanding of written or spoken English.
• Iron infusion within 1 week of 68Ga-DFO PET/CT scan (can potentially hamper the sensitivity of DFO by decreasing the number of siderophore receptors in the bacteria).

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

King's College London

OTHER

Sponsor Role: collaborator

Guy's and St Thomas' NHS Foundation Trust

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Sally Barrington, MD

Role: PRINCIPAL_INVESTIGATOR

King's College London

Locations

Kings College London and Guy's and St Thomas' Hospital PET Centre

London, , United Kingdom

Site Status: RECRUITING

Countries

United Kingdom

Central Contacts

Sally F Barrington, MD

Role: CONTACT

sally.barrington@kcl.ac.uk

02071884988

Facility Contacts

Sally Barrington, MD

Role: primary

sally.barrington@kcl.ac.uk

02071884988

Other Identifiers

271229

Identifier Type: -

Identifier Source: org_study_id