Significance of Cortactin Expression in Colorectal Adenocarcinomas

NCT ID: NCT05225870

Last Updated: 2022-07-15

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 50 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2021-01-01
• Study Completion Date: 2021-12-30

Brief Summary

Colorectal carcinoma is one of the most aggressive malignant epithelial neoplasms affecting the gastrointestinal tract. The incidence of colorectal carcinoma is obviously increasing in developing countries, where the physical inactivity and the consumption of animal fat-rich food became more evident. Colorectal tumorigenesis is a multistep process which is initiated by adenoma and is terminated by carcinoma, the latter shows variable degrees of tumor differentiation and invasiveness. During the adenoma-carcinoma process; a series of genetic mutations occur. Detection of these genetic mutations will help in the development of novel therapeutic agents, which in turn will improve patients' outcomes. Cortactin (CTTN) is a Src kinase substrate, encoded by a gene located on chromosome 11. CTTN binds to and activates Arp 2/3 and stabilizes the dynamic actin assembly after its formation. So, it become clear that CTTN is involved in the formation of the leading-edges cellular protrusions.

Detailed Description

Colorectal carcinoma is a highly lethal malignant epithelial tumor involving humans. The incidence of colorectal carcinoma is steadily increasing in developing countries, this could be assigned to low physical activity together with high consumption of animal fat-rich food. malignancy of the colon is a multistep process which is initiated by adenoma that proceeds to carcinoma, the latter includes a wide variety of tumor phenotypes. During the adenoma-carcinoma process; a series of genetic and epigenetic alterations occur. Detection of these genetic alterations is of great benefit in the future management of colorectal carcinomas. Cortactin (CTTN) is a Src kinase substrate, encoded by a gene located on chromosome 11. CTTN anchors the dynamic actin assembly . CTTN is implicated in the formation of the leading-edges cellular protrusions.the aim of this study is to evaluate expression of CTTN in 50 cases of colorectal adenocarcinomas and their adjacent normal colonic mucosae.

Conditions

Cancer Colon

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

patients with colorectal carcinoma

patients with colorectal carcinoma will undergo colectomy. their colectomy specimens will be sectioned, tissue blocks will be prepared from tumor and adjacent normal mucosa. sections will be stained immunohistochemically by antibody against cortactin.

Group Type: OTHER

immunohistochemical staining

Intervention Type: GENETIC

sections from colorectal carcinoma will be immunohistochemically stained by anti-Cortactin antobody.

Interventions

immunohistochemical staining

sections from colorectal carcinoma will be immunohistochemically stained by anti-Cortactin antobody.

Intervention Type: GENETIC

Eligibility Criteria

Inclusion Criteria

• patients with colorectal carcinomas who underwent colectomy operations.

Exclusion Criteria

• specimens obtained by lower endoscopy only, patients who received pre-operative chemotherapy.

• Minimum Eligible Age: 30 Years
• Maximum Eligible Age: 80 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Sohag University

OTHER

Sponsor Role: lead

Responsible Party

Maisa Hashem Mohammed

Lecturer of Pathology

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Maisa H Mohammed

Role: PRINCIPAL_INVESTIGATOR

a lecturer of pathology, Sohag faculty of medicine

Locations

Maisa Hashem Mohammed

Sohag, , Egypt

Countries

Egypt

Other Identifiers

Soh-Med-22-01-33

Identifier Type: -

Identifier Source: org_study_id