Determination of the Biological Activity of Serum From Patients

NCT ID: NCT04696718

Last Updated: 2022-08-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

20 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-12-20

Study Completion Date

2023-12-28

Brief Summary

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The main objective of the study is to determine the influence of patient sera before and after probiotic intervention on the behavior of bone cells (osteoblasts and osteoclasts).

The secondary objectives are to assess the activity of bone cells and the course of rheumatoid arthritis before and after the consomption of probiotics.

Detailed Description

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The written consent of the subjects will be obtained after full information of the aims, nature and possible risks of the study.

Before inclusion, subjects will undergo a medical check-up including an interview on personal and family history and drug treatments taken as well as a standard medical examination including weight, height and blood pressure measurement. . Their compliance with the inclusion / exclusion criteria will be verified during this review. An interview with personnel involved in the research (a dietician / study Research clinic assistant, etc.) will also be carried out as well as a biological selection assessment.

As part of this study, the subjects will come a total of 7 times to the Emile ROUX hospital in Le Puy en Velay.

The probiotic treatment will begin between 1 and 3 weeks after the inclusion visit. The volunteers / patients recruited will have a volume of blood sampled and a stool collected before the start of the probiotic treatment (T = 0) and at the end of the treatment (T = 4 weeks). The study consists of three periods of probiotic treatment of 4 weeks each interspersed with a wash-out period of 3 weeks in accordance with the recovery period necessary between two blood samples.

The sera thus obtained will then be analyzed to determine their biological activity with regard to the induction of changes in the behavior of bone cell models: osteoformin cells (osteoblasts) and osteo-resorbent cells (osteoclasts).

Conditions

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Women Rheumatoid Arthritis

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

CROSSOVER

Crossover Assignment patient may receive 3 products in 3 interventions sequentially during the protocol
Primary Study Purpose

PREVENTION

Blinding Strategy

SINGLE

Participants
single blind

Study Groups

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Traitement 1

Lactobacillus salivarius

Group Type EXPERIMENTAL

daily supplementation

Intervention Type DIETARY_SUPPLEMENT

the patients will consume 1 pill of probiotic each day for 28 days per treatments each treatment is separated by a wash out period of 21 days

Traitement 2

Lactobacillus Rhamnosus GG

Group Type EXPERIMENTAL

daily supplementation

Intervention Type DIETARY_SUPPLEMENT

the patients will consume 1 pill of probiotic each day for 28 days per treatments each treatment is separated by a wash out period of 21 days

Traitement 3

Bifidobacterium lactis

Group Type EXPERIMENTAL

daily supplementation

Intervention Type DIETARY_SUPPLEMENT

the patients will consume 1 pill of probiotic each day for 28 days per treatments each treatment is separated by a wash out period of 21 days

Interventions

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daily supplementation

the patients will consume 1 pill of probiotic each day for 28 days per treatments each treatment is separated by a wash out period of 21 days

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

Women

* 30-75 years
* Non-menopausal or menopausal for more than 2 years because the tissue osteoarticular is under the influence of steroid hormones. Peri-menopause is accompanied by strong hormonal variations.
* With rheumatoid arthritis (DAS28 threshold specified below)
* Clinical arthritis score ≥ 2.6 (DAS28). Pathology needs to be characterized even if the score remains low
* Person with good venous condition

Exclusion Criteria

Vaccination in the last two months

* Alcohol abuse with regard to WHO standards
* Smoking (\>5 cigarettes/day)

-\> 5 hours of intense sport per week
* Food allergy and others
* Antibiotic treatment in the month preceding inclusion
* Persons under guardianship, curators, deprived of liberties, safeguard of justice
* Refusal to sign the information and consent form
Minimum Eligible Age

30 Years

Maximum Eligible Age

75 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Centre Hospitalier Emile Roux

OTHER

Sponsor Role collaborator

Centre de Recherche en Nutrition Humaine d'Auvergne

OTHER_GOV

Sponsor Role collaborator

Adeline BLOT

OTHER

Sponsor Role lead

Responsible Party

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Adeline BLOT

Clinical Research Associate

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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benjamin castagne, MD

Role: PRINCIPAL_INVESTIGATOR

Centre Hospitalier Emile Roux

Locations

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Centre Hospitalier Emile Roux

Le Puy-en-Velay, , France

Site Status RECRUITING

Countries

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France

Central Contacts

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yohann wittrant, PhD

Role: CONTACT

+33682297271

emilie gadea, PhD

Role: CONTACT

Facility Contacts

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emile gadea, PhD

Role: primary

Other Identifiers

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2018-A00303-52

Identifier Type: -

Identifier Source: org_study_id

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