DNA-guided Second Line Adjuvant Therapy for High Residual Risk, Estrogen Receptor Positive, HER-2 Negative Breast Cancer (DARE)

NCT ID: NCT04567420

Last Updated: 2025-10-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE2

Total Enrollment

70 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-02-09

Study Completion Date

2028-12-31

Brief Summary

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A randomized, Phase II trial of circulating tumor DNA-guided second line Adjuvant therapy for high Residual risk, Estrogen Receptor positive, HER-2 negative breast cancer (DARE)

Detailed Description

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Surveillance population and ctDNA screening (up to 1000 patients): High risk ER positive, HER2-, breast cancer patients who have completed adjuvant endocrine therapy, or are currently receiving adjuvant endocrine therapy with an aromatase inhibitor or tamoxifen and are within 7 years since completion of definitive breast surgery are eligible for ctDNA screening.

In order to start ctDNA surveillance, patients must have completed at least 6 months, but no more than 7 years of adjuvant endocrine therapy of treatment without distant recurrence. Prior adjuvant CDK4/6 therapy is allowed, but at least 12 months must have elapsed since completing CDK4/6 therapy and enrolling into ctDNA surveillance on this study. Participants in the PENELOPE and PALLAS clinical trials who received Palbociclib are also eligible if meet all required eligibility criteria.

For screening, patients will undergo Signatera testing during routine follow up clinic visits. The current ASCO/NCCN breast cancer practice guidelines recommend follow up visits every 4 to 6 months at the treating physician's discretion. We anticipate that screening positivity rates will be the highest in patients between years 1-5 after initial diagnosis, based on the annual hazard rates of recurrence in ER positive breast cancer.

However, since up to 50% of all recurrences occur after 5 years of follow-up, we allow starting ctDNA screening up to 7 years after starting adjuvant endocrine therapy if a patient meets criteria for high risk.

Randomized phase II (N=100): Patients who become ctDNA positive during ctDNA surveillance will have systemic staging with CT of the chest, abdomen and pelvis, and those without radiographic evidence of metastatic disease will be randomized 1:1 to receive palbociclib plus fulvestrant for two years or continue standard of care endocrine therapy. Pre- and peri- menopausal patients randomized to the fulvestrant palbociclib arm will require GnRH analogue therapy. Patients in both treatment arms may continue adjuvant bisphosphonate therapy and patients in the control arm may switch between different brands of aromatase inhibitors for better tolerance or patient preference. No other, non-protocol directed anticancer therapy is allowed. The maximum duration of treatment is 2 years. A patient may complete a maximum of 26 cycles of treatment (for patients without interruptions or delays). The goal is not to administer a specific number of cycles, but to allow for the completion of any cycles initiated prior to 2 years from randomization. Patients who have completed 2 years of fulvestrant and palbociclib without recurrence may resume their originally planned standard of care adjuvant therapy to complete a total of 5 or 10 years of endocrine therapy at the discretion of the treating physician.

Conditions

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Breast Cancer

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Arm A

Palbociclib/Fulvestrant Combination

Group Type EXPERIMENTAL

Palbociclib

Intervention Type DRUG

4 week cycles

Fulvestrant

Intervention Type DRUG

4 week cycles

Arm B

Adjuvant Therapy

Group Type ACTIVE_COMPARATOR

Adjuvant Therapy

Intervention Type DRUG

Standard of Care

Interventions

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Palbociclib

4 week cycles

Intervention Type DRUG

Fulvestrant

4 week cycles

Intervention Type DRUG

Adjuvant Therapy

Standard of Care

Intervention Type DRUG

Other Intervention Names

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IBRANCE Faslodex Standard of Care

Eligibility Criteria

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Inclusion Criteria

1. High risk for recurrence HER-2 negative, ER positive invasive breast cancer. For this study, ER positivity is defined as equal to or greater than 10% ER positivity by immunohistochemistry, regardless of progesterone receptor (PR) status. Patients who are PR positive but ER negative are not eligible.
2. Patients may have completed adjuvant endocrine therapy and are within 7 years since the date of their definitive breast surgery, or may be currently taking an aromatase inhibitor, or tamoxifen, as adjuvant endocrine therapy and have completed at least 6 months (i.e. 24 weeks), but no more than 7 years of endocrine therapy. Prior CDK4/6 therapy in the adjuvant setting, including participation in the PALLAS and PENELOPE trials, is allowed if the last treatment was 12 or more months ago. Adjuvant bisphosphonate therapy is allowed
3. High risk for recurrence is defined as any one of the following (these criteria apply equally to both patients who underwent surgery first and those who received neoadjuvant chemotherapy or endocrine therapy before surgery). (i) Four or more involved ipsilateral axillary lymph nodes or positive ipsilateral supraclavicular, or ipsilateral infraclavicular, or internal mammary lymph nodes at diagnosis or after preoperative systemic therapy, regardless of tumour size. Microscopic positive lymph node (i.e. \<2 mm tumor deposit) is not counted as positive for eligibility for patients who underwent surgery first without any preoperative systemic therapy. Microscopic positive lymph nodes (i.e. \<2 mm tumor deposit) are considered as positive nodes for eligibility for patients who received preoperative systemic therapy. (ii) Tumor size \>5 cm and at least one macroscopically positive lymph node (i.e. \>2 mm tumor deposit).

(iii) Diagnosis of Inflammatory Breast Cancer.
4. Formalin fixed paraffin embedded tissue from the primary breast cancer available to be sent to Natera to perform ctDNA testing.
5. Signed and dated informed consent, including willingness to be randomized to standard of care versus fulvestrant + palbociclib.


1. ctDNA positivity by the Signatera assay, defined as 2 of the 16 cancer specific markers positive in plasma.
2. Patients with positive Signatera results obtained in the context of commercial testing, outside of the screening phase of this trial, are also eligible for randomization if they meet other eligibility criteria.
3. No evidence of metastatic disease on CT scan of the chest, abdomen and pelvis.

1. If imaging, after review with a radiologist, is low probability for metastatic disease, patients may proceed to randomization.
2. Patients with suspicious but inconclusive imaging results should undergo a diagnostic biopsy, if biopsy is negative patients are eligible for randomization.
3. Patients with positive imaging that is conclusive of metastatic disease, or with biopsy proven metastatic disease, are not eligible for randomization.
4. Pre-menopausal women and male patients must be willing to use an adequate method of contraception for the duration of trial treatment and for 4 additional weeks after completion of treatment in the control arm, and for 2 years after the last dose of fulvestrant, if randomized into the experimental arm Post- menopausal status is defined as:

1. Documented bilateral oophorectomy, or
2. Age ≥ 60 years, or
3. Age \< 60 years and amenorrhoeic for ≥ 12 consecutive months and FSH and estradiol levels in the post-menopausal range according to the institutional reference range for post- menopausal.

Adequate contraception is defined as:
4. ONE highly effective form (i.e. abstinence, surgical sterilization through bilateral tubal ligation, vasectomy), or
5. TWO effective forms (e.g. non-hormonal IUD and condom / occlusive cap with spermicidal foam / gel / film / cream / suppository).
6. Abstinence is to be interpreted as "true abstinence" for heterosexual intercourse and therefore, "periodic abstinence" (e.g. calendar, symptothermal, post-ovulation methods) and withdrawal (coitus interruptus) are not considered highly effective.

Exclusion Criteria

1. Prior or current treatment with fulvestrant, or current treatment with a CDK4/6 inhibitor, or treatment in the prior 12 months.
2. Patients cannot start participation in another therapeutic clinical trial for breast cancer during participation in this trial unless disease progression occurred, or patient withdrew consent for participation in the current trial.
3. Patients with current or past invasive cancer, other than breast cancer are not eligible, except: Adequately treated basal or squamous cell carcinoma of the skin and cancer survivors of previously diagnosed invasive cancer, who were treated with a curative intent, have no evidence of disease recurrence for 5 years or more, and are considered low risk for future recurrence by the treating physician are also eligible.
4. Patients with a second HER2 positive or triple negative synchronous breast cancer.


1. Patients with known contraindications to receive fulvestrant and palbociclib or those who are unable to tolerate these drugs are not eligible (e.g. absolute neutrophil count less than \<1000/mm3)
2. Any concurrent severe and uncontrolled medical condition that would, in the Investigator' opinion cause unacceptable safety risks or compromise compliance with the protocol including but not limited to:

1. Impairment of gastrointestinal (GI) function or GI disease that may significantly alter the absorption of oral medication (e.g. Crohn's disease, ulcerative diseases, uncontrolled chronic nausea, vomiting, diarrhea, malabsorption syndrome, or small bowel resection)
2. History of pneumonitis, interstitial lung disease or pulmonary fibrosis
3. Known history of Human Immunodeficiency Virus (HIV) (testing is not mandatory)
4. Known active Hepatitis B or Hepatitis C (testing is not mandatory)
5. Females who are pregnant or breastfeeding
6. History of bleeding diathesis (i.e. disseminated intravascular coagulation, clotting factor deficiency) that preclude the IM injections of fulvestrant or LHRH agonist as applicable.
3. Patients taking any CYPC3A4 strong inducers and inhibitors, that cannot be changed.
Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Criterium, Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Lajos Pusztai, MD

Role: PRINCIPAL_INVESTIGATOR

Yale University

Locations

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University of Arizona Cancer Center

Tucson, Arizona, United States

Site Status RECRUITING

USC/Norris Comprehensive Cancer Center

Los Angeles, California, United States

Site Status RECRUITING

Cedars-Sinai Medical Center

Los Angeles, California, United States

Site Status COMPLETED

University of Colorado Cancer Center

Aurora, Colorado, United States

Site Status RECRUITING

Intermountain

Golden, Colorado, United States

Site Status RECRUITING

Yale Cancer Center

New Haven, Connecticut, United States

Site Status RECRUITING

Winship Cancer Institute of Emory University

Atlanta, Georgia, United States

Site Status RECRUITING

Louisiana State University Health Sciences Center- New Orleans

New Orleans, Louisiana, United States

Site Status RECRUITING

Cancer Partners of Nebraska

Lincoln, Nebraska, United States

Site Status RECRUITING

New Mexico Cancer Care Alliance

Albuquerque, New Mexico, United States

Site Status RECRUITING

Icahn School of Medicine at Mount Sinai

New York, New York, United States

Site Status RECRUITING

Stony Brook University Cancer Center

Stony Brook, New York, United States

Site Status RECRUITING

The Ohio State University Wexner Medical Center James Cancer Hospital

Columbus, Ohio, United States

Site Status RECRUITING

Stephenson Cancer Center

Oklahoma City, Oklahoma, United States

Site Status RECRUITING

Oregon Health and Science University

Portland, Oregon, United States

Site Status RECRUITING

PRiSMs Group

Laredo, Texas, United States

Site Status RECRUITING

Virginia Cancer Institute

Richmond, Virginia, United States

Site Status RECRUITING

Swedish Cancer Institute

Seattle, Washington, United States

Site Status RECRUITING

University of Wisconsin Clinical Science Center

Madison, Wisconsin, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Bebi Yassin-Rajkumar, MSc

Role: CONTACT

6138511370

Julee Hartwell

Role: CONTACT

607-651-6273

Facility Contacts

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Alexia Demitsas, MS

Role: primary

520-694-9089

Grace Facio

Role: primary

323-409-7027

Leah Adams

Role: primary

720-848-7341

Melissa Cross

Role: backup

720-848-8031

Austin Daw

Role: primary

303-403-6381

Carl Brown

Role: primary

Ogochukwu Chukudi

Role: primary

404-778-1832

Ashley Trumbull

Role: backup

404-778-3969

Michelle Loch, MD

Role: primary

504-568-2346

Sheila Evans

Role: primary

Ellen R Wojcik

Role: primary

Esther Kim

Role: primary

212-824-7193

Alison Stopeck, MD

Role: primary

631-728-7425

Pushpa Talanki

Role: backup

631-638-0815

Christina Hayduchok

Role: primary

614-685-4852

Jennifer Fritts

Role: primary

405-271-8001 ext. 48686

Jeanette Phillips

Role: primary

Role: backup

971-262-9041

Ekta Grewal

Role: primary

Stacey Wright

Role: primary

Kim Reeves

Role: primary

206-215-2804

UW Cancer Connect

Role: primary

608-262-5223

Other Identifiers

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DARE

Identifier Type: -

Identifier Source: org_study_id

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