Epidemiological Study of Seroprevalence Against the SARS-CoV-2 Virus (COVID-19)

NCT ID: NCT04448769

Last Updated: 2020-08-19

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 2006 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2020-06-26
• Study Completion Date: 2020-07-24

Brief Summary

In order to inform the public decision on the containment strategy and knowledge of the intensity of the epidemic during post-containment, estimates of the share of the population infected with the SARS-CoV-2 virus responsible for COVID-19 disease at the territorial level are needed as soon as possible. The aim of the study is to estimate the prevalence of positive anti-SARS-CoV-2 serologies by detection of IgT-total antibodies (IgM/IgA/IgG) in the general population of the Grand Nancy Metropolitan area.

A study of seroprevalence and symptom collection, or absence of symptoms, on a cluster (household) sample of the Grand Nancy Metropolitan population randomly selected will be conducted.

The target population consists of all the inhabitants of the Grand Nancy Metropolitan area, from which a sample is drawn from the electoral lists (households) in a random manner to ensure representativeness. In order to ultimately include 2000 people in the study

Detailed Description

In order to inform the public decision on the containment strategy and knowledge of the intensity of the epidemic during post-containment, estimates of the share of the population infected with the SARS-CoV-2 virus responsible for COVID-19 disease at the territorial level are needed as soon as possible. To date, these estimates are based on RT-qPCR tests carried out on individuals with symptoms of the disease, on people likely to present severe forms of COVID-19, known as 'vulnerable', or on the number of hospitalizations/deaths related to COVID-19. In order to refine these estimates, it is crucial to carry out measurements on a random sample of the population and to take biological samples for serological testing, in accordance with the recommendations of the HAS and the scientific societies.

Besides, it is important to assess the proportion of persons reporting that they have not developed symptoms of COVID-19 since the beginning of the epidemic among seropositive persons.

The aim of the study is to estimate the prevalence of positive anti-SARS-CoV-2 serologies by detection of IgT-total antibodies (IgM/IgA/IgG) in the general population of the Grand Nancy Metropolitan area.

A study of seroprevalence and symptom collection, or absence of symptoms, on a cluster (household) sample of the GNM population randomly selected.

It is a cross-sectional, not experimental study in the sense that it does not alter the exposure of participants; however, it does require a blood sample, which classifies it as minimal risk intervention research according to French law. The study is monocentric.

The target population consists of all the inhabitants of the Grand Nancy Metropolitan area, from which a sample is drawn from the electoral lists (households) in a random manner to ensure representativeness. In order to ultimately include 2000 people in the study.

Each inhabitant who agrees to participate will have to :

• sign the consent form after receiving complete information
• complete a questionnaire exploring sociodemographic (gender, age, professional activity...), medical (anthropometric measures, health problems, smoking...), potential contacts with COVID-19 and symptoms.
• take a blood sample:

• in one of the identified locations of the GNM after making an appointment with the dedicated secretariat.
• at their homes for those vulnerable to Covid-19. The total duration of participation of a subject will be ½ day (collection of consent + filling in the questionnaire and blood sampling)

Data are collected from :

• the electoral lists: surname, first names, gender, address allowing identification of the IRIS zone
• self-report questionnaires (adapted to the age : an adult questionnaire, a child questionnaire completed by the parents and an adolescent questionnaire) : socio-demographic characteristics of the respondent : age, sex, professional activity, socio-professional category, level of education ; EPICES questionnaire (adult), FAS questionnaire (child) ; medical characteristics of the respondent : weight, height, smoking status, influenza vaccination, health problems, pregnancy; potential contacts with COVID-19: perception of having been infected by the coronavirus, close relatives having been infected; potential symptoms: exploration of symptoms felt since mid-February.
• serological test results: Anti-SARS-CoV-2 IgT seropositivity

The statistical analysis concerns all participants who respond to the questionnaire (even if blood sample could not be collected); it does not include any intermediate results and there is no statistical criterion for stopping the research.

The following analyses will be carried out :

• Proportions: All proportions (including seroprevalence) will have confidence intervals calculated with the approximation by the normal distribution when the observed proportion and the size of the study group allow it, otherwise by the exact calculation of the binomial distribution.
• Risk groups: Standardized prevalences for age and sex and their confidence intervals will be given for each group of each risk factor (smoking status, weight status, level of insecurity). Significance (p-value) will be calculated by a chi-square test if the size of the subgroups allows it, a Fisher test otherwise. A relationship will be significant if p<0.05
• Analysis of the serology result: The ELISA method allows semi-quantitative detection of total IgT antibodies. A positive sample will be defined by a ratio ≥ 1.0.
• Intra-household transmission: The share of infections due to intra-household transmission will be estimated using a Bayesian model.

• - Symptom expression groups: In seropositive participants, an exploratory study will be conducted to determine whether there are typologies of symptom expression .

This type of survey should make it possible to estimate the penetration of the virus in a very geographically targeted population (GNM area) over a short period of time and to evaluate the role of households in the transmission of SARS-CoV-2.

This measurement, based on a random sample, could make it possible to consolidate estimates obtained from molecular biology data (qRT-PCR) or the number of hospitalizations and/or deaths.

In addition, in vitro neutralization tests will allow an initial assessment of the ability of the detected antibodies to protect against SARS-CoV-2.

Combined with other similar studies (COVAL Paris, Epicov, ...) it will make it possible to measure the heterogeneity of virus penetration at the national level and to consolidate symptom association profiles.

All of the results (detection of cases of infection, particularly those that have not experienced symptoms, estimation of the number of people protected against a new infection (neutralisation results)) will in turn feed into mathematical models of the SEIR type (Susceptible, Exposed, Infected, Recovered).

Conditions

Coronavirus Infection; Prevalence

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: SCREENING
• Blinding Strategy: NONE

Study Groups

Anti-SARS-CoV-2 IgT seropositivity

Analysis of the serology result: The ELISA method allows semi-quantitative detection of total IgT antibodies. A positive sample will be defined by a ratio ≥ 1.0.

Group Type: OTHER

Anti-SARS-CoV-2 IgT seropositivity

Intervention Type: BIOLOGICAL

Each inhabitant who agrees to participate will have to :

• sign the consent form after receiving complete information
• complete a questionnaire exploring sociodemographic (gender, age, professional activity...), medical (anthropometric measures, health problems, smoking...), potential contacts with COVID-19 and symptoms.
• take a blood sample for the dermination of anti-SARS-CoV-2 IgT seropositivity of the individuals tested in the population of the Grand Nancy Metropolitan area (GNM).

Interventions

Anti-SARS-CoV-2 IgT seropositivity

Each inhabitant who agrees to participate will have to :

• sign the consent form after receiving complete information
• complete a questionnaire exploring sociodemographic (gender, age, professional activity...), medical (anthropometric measures, health problems, smoking...), potential contacts with COVID-19 and symptoms.
• take a blood sample for the dermination of anti-SARS-CoV-2 IgT seropositivity of the individuals tested in the population of the Grand Nancy Metropolitan area (GNM).

Intervention Type: BIOLOGICAL

Eligibility Criteria

Inclusion Criteria

• Person who has received full information about the research organization and signed informed consent
• Person residing in the Grand Nancy Metropolitan area
• Person aged at least 5 years on 1 June 2020 and weighing more than 7 kg

Exclusion Criteria

• Children under 5 years of age at the time of collection
• Person referred to in Articles L1121-8 of the Public Health Code. A person of full age who is subject to a legal protection measure (guardianship, curatorship, legal protection).

Adult person unable to give consent

• Persons deprived of their liberty by a judicial or administrative decision, persons under psychiatric care pursuant to articles L. 3212-1 and L. 3213-1.

• Minimum Eligible Age: 5 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

University of Lorraine

OTHER

Sponsor Role: collaborator

Métropole du Grand Nancy

UNKNOWN

Sponsor Role: collaborator

Central Hospital, Nancy, France

OTHER

Sponsor Role: lead

Responsible Party

Evelyne Schvoerer

Pr

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Evelyne Schvoerer

Role: PRINCIPAL_INVESTIGATOR

CHRU Nancy

Locations

Chru Nancy

Vandœuvre-lès-Nancy, , France

Countries

France

Other Identifiers

2020PI128

Identifier Type: -

Identifier Source: org_study_id