Skin Barrier Assessment in Pregnancy and at Birth

NCT ID: NCT04445298

Last Updated: 2020-09-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

160 participants

Study Classification

OBSERVATIONAL

Study Start Date

2020-08-03

Study Completion Date

2022-07-01

Brief Summary

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This is a prospective birth cohort study assessing the role of maternal and environmental factors on the development of allergic diseases in children. Pregnant mothers will be enrolled and we will examine her skin barrier with skin tape strips (STS) and transepidermal water loss (TEWL), along with blood work. We will then follow her offspring and perform similar testing, along with detailed questionnaires inquiring about exposures such as use of detergents and soaps, sunlight exposure, and pollution exposure. When the infant is around 12 months old, we will contact the family via telephone to see if the child developed any allergic conditions within their first year of life, such as eczema, food allergy, or wheezing. A final questionnaire will be performed.

Detailed Description

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Conditions

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Pregnancy Related Allergy Atopic Dermatitis Eczema Asthma in Children Food Allergy

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Pregnant women with expected delivery in the fall or winter

We will enroll up to 40 women who are expected to deliver in the fall (September, October, November) and winter (December, January, February). We will then follow their infant offspring.

Transepidermal water loss

Intervention Type DIAGNOSTIC_TEST

We will assess the skin with transepidermal water loss (TEWL) by using a condenser-chamber system placed lightly on the skin. This will be performed at baseline, then after every 4 skin tape strips.

Skin tape stripping

Intervention Type DIAGNOSTIC_TEST

Adhesive skin sampling discs will be pressed against the skin in a hairless location followed by lifting it free of the skin. The discs will be evaluated for lipid content, protein, and filaggrin byproducts in the collected skin sample.

Blood draw

Intervention Type DIAGNOSTIC_TEST

We will draw blood to look at the vitamin D, total immunoglobulin E (IgE), and the filaggrin gene which is important in maintaining the skin barrier.

Bacterial PCR swab

Intervention Type DIAGNOSTIC_TEST

Skin swab from the superficial skin will be collected to be assessed in the laboratory via PCR for the presence of staphylococcus aureus.

Pregnant women with expected delivery in the spring or summer

We will enroll up to 40 women who are expected to deliver in the spring (March, April, May) and summer (June, July, August). We will then follow their infant offspring.

Transepidermal water loss

Intervention Type DIAGNOSTIC_TEST

We will assess the skin with transepidermal water loss (TEWL) by using a condenser-chamber system placed lightly on the skin. This will be performed at baseline, then after every 4 skin tape strips.

Skin tape stripping

Intervention Type DIAGNOSTIC_TEST

Adhesive skin sampling discs will be pressed against the skin in a hairless location followed by lifting it free of the skin. The discs will be evaluated for lipid content, protein, and filaggrin byproducts in the collected skin sample.

Blood draw

Intervention Type DIAGNOSTIC_TEST

We will draw blood to look at the vitamin D, total immunoglobulin E (IgE), and the filaggrin gene which is important in maintaining the skin barrier.

Bacterial PCR swab

Intervention Type DIAGNOSTIC_TEST

Skin swab from the superficial skin will be collected to be assessed in the laboratory via PCR for the presence of staphylococcus aureus.

Infants born in the fall or winter

The infants born to the enrolled mothers will be followed. These are infants born in the fall (September, October, November) or winter (December, January, February).

Transepidermal water loss

Intervention Type DIAGNOSTIC_TEST

We will assess the skin with transepidermal water loss (TEWL) by using a condenser-chamber system placed lightly on the skin. This will be performed at baseline, then after every 4 skin tape strips.

Skin tape stripping

Intervention Type DIAGNOSTIC_TEST

Adhesive skin sampling discs will be pressed against the skin in a hairless location followed by lifting it free of the skin. The discs will be evaluated for lipid content, protein, and filaggrin byproducts in the collected skin sample.

Blood draw

Intervention Type DIAGNOSTIC_TEST

We will draw blood to look at the vitamin D, total immunoglobulin E (IgE), and the filaggrin gene which is important in maintaining the skin barrier.

Bacterial PCR swab

Intervention Type DIAGNOSTIC_TEST

Skin swab from the superficial skin will be collected to be assessed in the laboratory via PCR for the presence of staphylococcus aureus.

Infants born in the spring or summer

The infants born to the enrolled mothers will be followed. These are infants born in the spring (March, April, May) or summer (June, July, August).

Transepidermal water loss

Intervention Type DIAGNOSTIC_TEST

We will assess the skin with transepidermal water loss (TEWL) by using a condenser-chamber system placed lightly on the skin. This will be performed at baseline, then after every 4 skin tape strips.

Skin tape stripping

Intervention Type DIAGNOSTIC_TEST

Adhesive skin sampling discs will be pressed against the skin in a hairless location followed by lifting it free of the skin. The discs will be evaluated for lipid content, protein, and filaggrin byproducts in the collected skin sample.

Blood draw

Intervention Type DIAGNOSTIC_TEST

We will draw blood to look at the vitamin D, total immunoglobulin E (IgE), and the filaggrin gene which is important in maintaining the skin barrier.

Bacterial PCR swab

Intervention Type DIAGNOSTIC_TEST

Skin swab from the superficial skin will be collected to be assessed in the laboratory via PCR for the presence of staphylococcus aureus.

Interventions

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Transepidermal water loss

We will assess the skin with transepidermal water loss (TEWL) by using a condenser-chamber system placed lightly on the skin. This will be performed at baseline, then after every 4 skin tape strips.

Intervention Type DIAGNOSTIC_TEST

Skin tape stripping

Adhesive skin sampling discs will be pressed against the skin in a hairless location followed by lifting it free of the skin. The discs will be evaluated for lipid content, protein, and filaggrin byproducts in the collected skin sample.

Intervention Type DIAGNOSTIC_TEST

Blood draw

We will draw blood to look at the vitamin D, total immunoglobulin E (IgE), and the filaggrin gene which is important in maintaining the skin barrier.

Intervention Type DIAGNOSTIC_TEST

Bacterial PCR swab

Skin swab from the superficial skin will be collected to be assessed in the laboratory via PCR for the presence of staphylococcus aureus.

Intervention Type DIAGNOSTIC_TEST

Other Intervention Names

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TEWL STS PCR

Eligibility Criteria

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Inclusion Criteria

• Pregnant women at any stage of a singleton pregnancy.


• Infant is the offspring of a woman enrolled in the study.

Exclusion Criteria

* Use of systemic immunosuppressive drugs including oral steroids within 30 days of skin barrier assessment
* Has received total body phototherapy (e.g., ultraviolet light B \[UVB\], psoralen plus ultraviolet light A \[PUVA\], tanning beds \[\>1 visit per week\]) within 30 days of skin barrier assessment
* Use of topical corticosteroids, topical immunomodulatory agents, or topical antibiotics on the extremity being evaluated within 7 days of skin barrier assessment
* Use of systemic antibiotics, antiparasitics, antivirals, or antifungals within 7 days, or antihistamines within 5 days of skin barrier assessment
* Has taken a bleach bath within 7 days of skin barrier assessment
* Use of emollients on the extremity being evaluated within 24 hours of skin barrier assessment
* Has taken a bath or shower on the day of the skin barrier assessment


* Pregnant women with high risk pregnancies.
* Pregnancy is from an egg donation.
* Pregnant women pregnant with more than one fetus.
* Pregnant women with psychiatric and developmental co-morbidities that would render them unable to provide informed consent or perform study-related procedures.
* AIDS and HIV infection.
* A fetus with chromosomal or congenital abnormalities, a heritable hematological disorder like thalassemia or sickle cell disease in the mother.


* Infant is delivered earlier than 34 weeks gestation.
* Infant is born with a significant birth defect or medical condition where enrollment in this study is not in the infant's best interest.
Minimum Eligible Age

0 Minutes

Maximum Eligible Age

55 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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National Jewish Health

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Donald Leung, MD PhD

Role: PRINCIPAL_INVESTIGATOR

National Jewish Health

Locations

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National Jewish Health

Denver, Colorado, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Jessica W Hui, MD

Role: CONTACT

3033981245

Facility Contacts

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Jessica Hui, MD

Role: primary

Other Identifiers

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HS3513

Identifier Type: -

Identifier Source: org_study_id

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