Host-pathogen Interactions During SARS-CoV-2 Infection

NCT ID: NCT04376476

Last Updated: 2023-04-05

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

140 participants

Study Classification

INTERVENTIONAL

Study Start Date

2020-05-05

Study Completion Date

2022-05-13

Brief Summary

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The new Severe acute respiratory syndrome coronavirus (SARS-CoV-2) named coronavirus disease 2019 (COVID-19) is currently responsible for a pandemic spread of febrile respiratory infections, responsible for a veritable global health crisis.

In adults, several evolutionary patterns are observed: i) a/pauci-symptomatic forms; ii) severe forms immediately linked to rare extensive viral pneumonia; and iii) forms of moderate severity, some of which progress to secondary aggravation (Day 7-Day 10). Children can be affected, but are more rarely symptomatic and severe pediatric forms are exceptional.

Like some other coronaviruses (SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV)), these differences in clinical expression could be based on a variability in the immunological response, notably either via inhibition of the type I interferon (IFN-I) response, or on the contrary an immunological dysregulation responsible for a "cytokine storm" associated with the aggravation. Little is known about the impact of these innate immune response abnormalities on the adaptive response. In addition, certain genetic factors predisposing to a state of "hyper-fragility" and certain viral virulence factors could also be predictive of the clinical response.

In this context, the main hypothesis is that the virological analysis and the initial biological and immunological profiles are correlated with the initial clinical presentation of COVID-19 infection. In particular, children forms and pauci-symptomatic disease in adults may be linked to a more robust innate immune response, including better production of IFN-I.

Detailed Description

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Conditions

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Infection, Coronavirus Severe Acute Respiratory Syndrome Coronavirus 2

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

OTHER

Blinding Strategy

NONE

Study Groups

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Children group E1

Children with confirmed asymptomatic or pauci-symptomatic COVID infection will be recruited in pediatric emergency departments, among siblings of COVID-19+ pediatric patients or through the blood collection centers set up by the occupational health services. A single visit will be scheduled at the hospital (for clinical examination, biology, immunology, virology measurements) and a phone call performed at day 14.

Group Type EXPERIMENTAL

Blood sample

Intervention Type BIOLOGICAL

blood samples will be taken as below: Group E1: At day 0 Group E2: At day 0, day 7, day 14 Group E3: At day 0

Low or upper respiratory tract sample

Intervention Type BIOLOGICAL

Low or upper respiratory tract sample will be collected in order to take virology measurements:

Group E1: At day 0 Group E2: At day 0, day 7, day 14 Group E3: At day 0

phone call

Intervention Type OTHER

phone calls will be performed to collect data regarding patients' symptoms at: Group E1: Day 14 Group E3: Day 14

Children group E2

Children with confirmed COVID-19 infection requiring hospitalization will be recruited within participating centers (mostly in emergency and intensive care units). Data will be recorded (clinical examination, biology, immunology, virology measurements) during their hospital stay (day 0, day 7, in case of worsening) and a phone call performed at day 14 (or onsite visit if patient still hospitalized).

Group Type EXPERIMENTAL

Blood sample

Intervention Type BIOLOGICAL

blood samples will be taken as below: Group E1: At day 0 Group E2: At day 0, day 7, day 14 Group E3: At day 0

Low or upper respiratory tract sample

Intervention Type BIOLOGICAL

Low or upper respiratory tract sample will be collected in order to take virology measurements:

Group E1: At day 0 Group E2: At day 0, day 7, day 14 Group E3: At day 0

Stool collection or fecal swab

Intervention Type BIOLOGICAL

The stool collection or fecal swab will be collected in order to take virology measurements:

Group E1: / Group E2: At day 0, day 7, day 14 Group E3: At day 0

Children group E3

Children with confirmed non-COVID-19 viral infection requiring hospitalization will be recruited within participating centers (mostly in intensive care units). At inclusion, data will be recorded (clinical examination, biology, immunology, virology measurements) and a phone call performed at day 14.

Group Type EXPERIMENTAL

Blood sample

Intervention Type BIOLOGICAL

blood samples will be taken as below: Group E1: At day 0 Group E2: At day 0, day 7, day 14 Group E3: At day 0

Low or upper respiratory tract sample

Intervention Type BIOLOGICAL

Low or upper respiratory tract sample will be collected in order to take virology measurements:

Group E1: At day 0 Group E2: At day 0, day 7, day 14 Group E3: At day 0

Stool collection or fecal swab

Intervention Type BIOLOGICAL

The stool collection or fecal swab will be collected in order to take virology measurements:

Group E1: / Group E2: At day 0, day 7, day 14 Group E3: At day 0

phone call

Intervention Type OTHER

phone calls will be performed to collect data regarding patients' symptoms at: Group E1: Day 14 Group E3: Day 14

Interventions

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Blood sample

blood samples will be taken as below: Group E1: At day 0 Group E2: At day 0, day 7, day 14 Group E3: At day 0

Intervention Type BIOLOGICAL

Low or upper respiratory tract sample

Low or upper respiratory tract sample will be collected in order to take virology measurements:

Group E1: At day 0 Group E2: At day 0, day 7, day 14 Group E3: At day 0

Intervention Type BIOLOGICAL

Stool collection or fecal swab

The stool collection or fecal swab will be collected in order to take virology measurements:

Group E1: / Group E2: At day 0, day 7, day 14 Group E3: At day 0

Intervention Type BIOLOGICAL

phone call

phone calls will be performed to collect data regarding patients' symptoms at: Group E1: Day 14 Group E3: Day 14

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

Group E1:

* Age from birth to \<18 years old;
* Weight\> 3 kilogram (kg);
* Infection with SARS-CoV-2 virus confirmed by RT-PCR on upper respiratory tract sample
* No fever or respiratory symptoms;
* Not requiring hospitalization (or hospitalization not related to a SARS-CoV-2 infection);
* Consent signed by at least one parent / holder of parental authority and assent of the child (if applicable);
* Beneficiary of a social security scheme

Group E2:

* Age from birth to \<18 years old;
* Weight\> 3kg;
* Infection with the SARS-CoV-2 virus confirmed by RT-PCR on a upper or low respiratory tract sample or pneumonia with scanner suggesting SARS-CoV-2 infection;
* Hospitalized in a pediatric intensive care unit or in a general pediatrics unit
* Consent signed by at least one parent / holder of parental authority and assent of the child (if applicable);
* Beneficiary of a social security scheme

Group E3:

* Age from birth to \<18 years old;
* Weight\> 3 kg;
* Negative SARS-CoV-2 PCR on at least one respiratory sample, and other confirmed viral infection
* Hospitalized in a pediatric intensive care unit or in a general pediatrics unit, for a respiratory reason;
* Consent signed by at least one parent / holder of parental authority and assent of the child (if applicable);
* Beneficiary of a social security scheme

Exclusion Criteria

Group E1:

* Patients with any other inherited or acquired immune deficiency that could compromise the immunological evaluation;
* Other Suspected or proved infection
* Pregnancy.

Group E2:

* Patients with any other inherited or acquired immune deficiency that could compromise the immunological evaluation;
* Pregnancy.

Group E3:

* Patients with any other inherited or acquired immune deficiency that could compromise the immunological evaluation;
* Infection with the SARS-CoV-2 virus known among the relatives
* Pregnancy.
Minimum Eligible Age

1 Day

Maximum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Hospices Civils de Lyon

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Groupement Hospitalier Nord-Daupine

Bourgoin, , France

Site Status

Hôpital femme-mère-enfant

Bron, , France

Site Status

Hôpital Louis Pradel

Bron, , France

Site Status

Hôpital Louis Mourier

Colombes, , France

Site Status

Centre Hospitalier D'Annecy-Genevois

Épagny, , France

Site Status

Centre Hospitalo-Universitaire de Grenoble

La Tronche, , France

Site Status

Hopital de la Croix-Rousse

Lyon, , France

Site Status

Hôpital Edouard Herriot

Lyon, , France

Site Status

Hôpital mère - enfant Nantes

Nantes, , France

Site Status

Centre Hospitalier Lyon Sud

Pierre-Bénite, , France

Site Status

Hôpital Nord de Saint Etienne

Saint-Priest-en-Jarez, , France

Site Status

Hôpital Nord-Ouest

Villefranche-sur-Saône, , France

Site Status

Countries

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France

Other Identifiers

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2020-A01102-37

Identifier Type: OTHER

Identifier Source: secondary_id

69HCL20_0342

Identifier Type: -

Identifier Source: org_study_id

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