Molecular Characteristics of Gastroesophageal Adenocarcinoma (MOCHA): A Prospective Feasibility Study

NCT ID: NCT04219137

Last Updated: 2025-05-14

Basic Information

• Recruitment Status: RECRUITING
• Total Enrollment: 120 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2019-11-14
• Study Completion Date: 2027-11-30

Brief Summary

Researchers are looking to further our knowledge on disease biology and treatment selection for gastroesophageal adenocarcinoma. The purpose of this study is to see how useful it is to look for changes and characteristics in your genes (molecules that contain instructions for the development and functioning of the cells) and the genes within the tumour. These characteristics may be useful in choosing treatments for patients for the future.

Detailed Description

There will be two study arms:

1. patients with suspected or diagnosed localized gastroesophageal adenocarcinoma (GEA) and

2. patients diagnosed with de novo metastatic gastroesophageal adenocarcinoma. Fresh tumour, adjacent normal tissue materials, and blood samples will be acquired and utilized to generate molecular data. Stool or rectal swab samples will also be acquired for microbiome analysis. Physiologic, quality of life, epidemiologic, frailty, and other clinical data will be systematically collected as standard of care to serve as clinical correlates for the molecular data.

Arm 1 Primary Objectives

1. Feasibility to produce a potential molecular signature in a clinically meaningful time point in patients with locally advanced GEA

2. Study the molecular characteristics of GEA in patients with localized and resectable disease and identify predictive signatures of response to induction therapy and the development of novel treatment regimens

3. To validate previously identified mutational signatures that defined subgroups of GEA

Arm 2 Primary Objectives

1. Feasibility to produce a potential molecular signature in a clinically meaningful time point for patients with advanced GEA on 1st line chemotherapy

2. Use of genotypes and genomic analyses to define therapies, and develop predictive and prognostic models

3. Assess the feasibility of prospectively identifying distinct genomic characteristics which associate with response to systemic therapy and survival

STUDY ENDPOINTS:

1. Feasibility of obtaining timely sequencing data (8-12 weeks) to guide treatment for patients progressing on 1st line treatment

2. Feasibility of using ctDNA, metabolome, immune profiling and other emerging technologies to guide treatment

3. Establish a program of personalized care for GEA patients in terms of pre-treatment assessment (Physiological and Frailty Risk Assessment, QOLQ, 4. Sarcopenia and Adiposity measurements) and treatment based on clinical-genomic correlations

4. Establishment of repository of biospecimens (tumour, blood and microbiome)

5. Establishment of robust preclinical models of GEA: PDO and PDX models

6. Assess the feasibility of using PDO models to identify drug sensitivity to guide treatment decisions

Conditions

Esophagogastric Adenocarcinoma

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: PROSPECTIVE

Study Groups

Localized Esophagogastric Adenocarcinoma

Patients diagnosed with gastroesophageal adenocarcinoma who will undergo surgical resection for curative intent, with or without neo-adjuvant chemotherapy or chemoradiotherapy.

Molecular Profiling

Intervention Type: GENETIC

This is a correlative study collecting biosamples to evaluate genomic characteristics and treatment outcomes of gastroesophageal adenocarcinoma.

Metastatic Esophagogastric Adenocarcinoma

Patients diagnosed with de novo metastatic gastroesophageal adenocarcinoma who will undergo platinum based first line chemotherapy.

Molecular Profiling

Intervention Type: GENETIC

This is a correlative study collecting biosamples to evaluate genomic characteristics and treatment outcomes of gastroesophageal adenocarcinoma.

Interventions

Molecular Profiling

This is a correlative study collecting biosamples to evaluate genomic characteristics and treatment outcomes of gastroesophageal adenocarcinoma.

Intervention Type: GENETIC

Other Intervention Names

Whole Genome Sequencing

Eligibility Criteria

Inclusion Criteria

Arm 1:

• Patients with suspected or histologically confirmed localized gastroesophageal adenocarcinoma amenable to curative intent therapy with surgery as standard of care, either with or without induction chemotherapy or chemo radiotherapy.
• Age ≥18 years.
• Eastern Cooperative Group (ECOG) performance status 0-2

Arm 2

• Patients must have a histological or radiological diagnosis of advanced gastroesophageal cancer.
• Patient must have a tumour lesion that is amenable to a core needle biopsy as judged by a staff radiologist. A minimum of 3 x 18G good quality tumour cores must be safely obtainable under CT or US guidance. Biopsy to be completed before systemic therapy begins.
• Patients must have a measurable lesion by RECIST 1.1 in addition to the lesion that is going to be biopsied. See Section 13.1.3 for the evaluation of measurable disease. Patients with locally advanced gastroesophageal cancer with no metastatic disease who are not candidates for curative intent therapy as per part 1 of the protocol, are eligible for part 2 and are exempt from this criterion.
• Patients must be fit enough to safely undergo a tumour biopsy as judged by the investigator.
• Age ≥ 18 years.
• Eastern Cooperative Group (ECOG) performance status 0-2
• Life expectancy of greater than 90 days, as judged by the investigator.
• Patients plan to undergo systemic treatment with platinum-based chemotherapy (e.g. FOLFOX, CF, CX with or without Herceptin) as first line standard systemic palliative treatment, or as part of a first line clinical trial.
• Within 14 days of the proposed biopsy date, patients must have normal organ and marrow functions.

Exclusion Criteria

Arm 1

• Patients who are planned for definitive chemoradiation without surgical resection will be excluded from this study.
• Any other condition that would, in the Investigator's judgment, contraindicate the patient's participation in the clinical study due to safety concerns or compliance with clinical study procedures.

Arm 2

• Patients with one or more contraindications to tumour biopsy according to UHN's standard biopsy procedures.
• Patients who had prior systemic treatment for advanced or metastatic gastroesophageal cancer.
• Patients who are currently on anti-cancer treatment including chemotherapy for another malignancy.
• Patients with known brain metastases are excluded from participation in this clinical study.
• Patients with advanced gastroesophageal cancer who are going to be treated with non-platinum based chemotherapy in the first line setting.
• Uncontrolled inter-current illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, or psychiatric illness/social situations that would limit compliance with study requirements.
• Any condition that would, in the investigators' judgment, contraindicate the patient's participation in the clinical study due to safety concerns or compliance with clinical study procedures.

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

University Health Network, Toronto

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Elena Elimova

Role: PRINCIPAL_INVESTIGATOR

University Health Network, Toronto

Locations

University Health Network

Toronto, Ontario, Canada

Site Status: RECRUITING

Countries

Canada

Central Contacts

Frances Allison

Role: CONTACT

Frances.Allison@uhn.ca

416-340-5446

Yvonne Bach

Role: CONTACT

Yvonne.Bach2@uhn.ca

416-340-5446

Facility Contacts

Elena Elimova

Role: primary

Elena.Elimova@uhn.ca

416-946-4501 ext. 2520

Other Identifiers

18-5663

Identifier Type: -

Identifier Source: org_study_id