Evaluating Selected Constituents in the Exhaled Breath Samples

NCT ID: NCT04143256

Last Updated: 2020-02-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

135 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-10-03

Study Completion Date

2019-12-13

Brief Summary

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An Open-Label, Multi-Center Study to Evaluate Selected Constituents in the Exhaled Breath Samples From the Use of JUUL Nicotine Salt Pod System Product (5% and 3% Virginia Tobacco, Mint, Mango, Menthol) Users and Conventional Cigarettes (Non-Menthol and Menthol Flavors)

Detailed Description

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The objective of this study is to evaluate the baseline-adjusted level of selected constituents in the exhaled breath sample (EBS) between the two JUUL NSPS products (5% vs 3% nicotine by weight) for Virginia Tobacco, Mint, Mango and Menthol flavor and to compare the change in absolute level of selected constituents in EBS before and after the use of JUUL NSPS (5% and 3% nicotine by weight; Virginia Tobacco, Mint, Mango, Menthol flavors) to the change in absolute level of selected constituents before and after the use of menthol and non-menthol cigarettes.

Subjects will be screened for participation in the trial up to 28 days before Day 1. Along with screening procedures, all subjects will receive training on EBS collection procedures. All subjects will be further trained on the CPS using a training video and site instructions. Trained subjects will have the opportunity to try the JUUL NSPS.

Eligible subjects will be scheduled for a Day 1 clinic visit. Within 24 to 48 hours prior to Check-in, the clinic staff will provide a courtesy phone call to subjects to remind them to abstain from the use of alcohol, any nicotine-containing products and mint/mentholated products for at least 12 hours prior and xanthines/caffeine for at least 24 hours prior to screening and Day 1.

Following completion of the check-in events, including re-instruction on the EBS sample collection technique and CPS instructions, subjects will participate in the exhaled breath collection test sessions.

Groups I - IV will complete two periods of baseline and test sample collections with a 3-hour break in between each period. Each period will consist of two baseline samples (the first for analysis of nicotine, propylene glycol and glycerin and the second for analysis of acetaldehyde, acrolein, benzoic acid, formaldehyde, and menthol), followed by product use, and then two test samples (similar to baseline). Exhaled CO will be measured before the first baseline sample, after the second baseline sample, before the first test sample and after the second test sample. Pods used during the test sessions will be weighed before and after use.

Groups V and VI will complete only one period of baseline and test sample collections, consisting of two baseline sample collections and two test sample collections similar to that noted above.

A follow-up phone call with subjects will be made the day after Check-out (or early withdrawal). Provided there are no adverse events which require further attention, the subject's participation in the trial will be complete.

Conditions

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Electronic Cigarette Use Cigarette Use, Electronic Tobacco Use Tobacco Smoking

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Four crossover groups and two single arm groups
Primary Study Purpose

OTHER

Blinding Strategy

NONE

Study Groups

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Group I

Subjects with ENDS user history may be randomized to use JUUL ENDS 5% Virginia Tobacco and 3% Virginia Tobacco

Group Type EXPERIMENTAL

JUUL ENDS 5% Virginia Tobacco

Intervention Type OTHER

JUUL ENDS Virginia Tobacco 5% nicotine strength

JUUL 3% Virginia Tobacco ENDS

Intervention Type OTHER

JUUL ENDS Virginia Tobacco 3% nicotine strength

Group II

Subjects with ENDS user history may be randomized to use JUUL ENDS 5% Mint and 3% Mint

Group Type EXPERIMENTAL

JUUL 5% Mint ENDS

Intervention Type OTHER

JUUL ENDS Mint 5% nicotine strength

JUUL 3% Mint ENDS

Intervention Type OTHER

JUUL ENDS Mint 3% nicotine strength

Group III

Subjects with ENDS user history may be randomized to use JUUL ENDS 5% Menthol and 3% Menthol

Group Type EXPERIMENTAL

JUUL 5% Menthol ENDS

Intervention Type OTHER

JUUL ENDS Menthol 5% nicotine strength

JUUL 3% Menthol ENDS

Intervention Type OTHER

JUUL ENDS Menthol 3% nicotine strength

Group IV

Subjects with ENDS user history may be randomized to use JUUL ENDS 5% Mango and 3% Mango

Group Type EXPERIMENTAL

JUUL 5% Mango ENDS

Intervention Type OTHER

JUUL ENDS Mango 5% nicotine strength

JUUL 3% Mango ENDS

Intervention Type OTHER

JUUL ENDS Mango 3% nicotine strength

Group V

Subjects with cigarette user history will be assigned to use US Cigarette, Non-Menthol Flavor (Marlboro Gold King Size)

Group Type EXPERIMENTAL

US Cigarette, Non-menthol - Marlboro Gold King Size

Intervention Type OTHER

US Cigarette, Non-menthol - Marlboro Gold King Size

Group VI

Subjects with cigarette user history will be assigned to use US Cigarette, Menthol Flavor (Newport King Size)

Group Type EXPERIMENTAL

US Cigarette, Menthol - Newport King Size

Intervention Type OTHER

US Cigarette, Menthol - Newport King Size

Interventions

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JUUL ENDS 5% Virginia Tobacco

JUUL ENDS Virginia Tobacco 5% nicotine strength

Intervention Type OTHER

JUUL 3% Virginia Tobacco ENDS

JUUL ENDS Virginia Tobacco 3% nicotine strength

Intervention Type OTHER

JUUL 5% Mint ENDS

JUUL ENDS Mint 5% nicotine strength

Intervention Type OTHER

JUUL 3% Mint ENDS

JUUL ENDS Mint 3% nicotine strength

Intervention Type OTHER

JUUL 5% Menthol ENDS

JUUL ENDS Menthol 5% nicotine strength

Intervention Type OTHER

JUUL 3% Menthol ENDS

JUUL ENDS Menthol 3% nicotine strength

Intervention Type OTHER

JUUL 5% Mango ENDS

JUUL ENDS Mango 5% nicotine strength

Intervention Type OTHER

JUUL 3% Mango ENDS

JUUL ENDS Mango 3% nicotine strength

Intervention Type OTHER

US Cigarette, Non-menthol - Marlboro Gold King Size

US Cigarette, Non-menthol - Marlboro Gold King Size

Intervention Type OTHER

US Cigarette, Menthol - Newport King Size

US Cigarette, Menthol - Newport King Size

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

1. Be a healthy male or female aged 21 - 65 years, inclusive, at the time of signing the informed consent.
2. Be informed of the nature of the study, agree to and are able to read, review, and sign the informed consent document prior to any study procedures. The informed consent document will only be available in English. Therefore, the volunteer must have the ability to read and communicate in English in order to participate in the study.
3. Complete the screening process up to 28 days prior to Day 1.
4. Satisfy the user definitions of either cigarette user or ENDS/closed system user.
5. Agree to abide by the study restrictions.
6. Agree to abstain from use of alcohol 12 hours before the start of Day 1 for Groups I through VI.
7. Agree to abstain from use of any nicotine products 12 hours before the start of study day 1 for Groups I through VI.
8. Agree to not consume any mint or menthol flavor products (e.g., chewing gum, mouth wash, toothpaste, etc.) 12 hours before the start of testing for Groups I through VI.
9. Have a positive urine cotinine result at screening of \>200 ng/ml.
10. A female subject of childbearing potential must have been using one (1) of the following forms of contraception and agree to continue using it through completion of the trial:

* hormonal (e.g., oral, vaginal ring, transdermal patch, implant, or injection) consistently for at least 3 months prior to Day 1;
* double barrier method (e.g., condom with spermicide, diaphragm with spermicide) at screening.
* intrauterine device for at least 3 months prior to Day 1;
* a partner who has been vasectomized for at least 6 months prior to Day 1;
* abstinence beginning at least 6 months prior to screening.
11. A female subject of non childbearing potential must have undergone one of the following sterilization procedures at least 6 months prior to Day 1:

* hysteroscopic sterilization;
* bilateral tubal ligation or bilateral salpingectomy;
* hysterectomy;
* bilateral oophorectomy;
* Or be postmenopausal with amenorrhea for at least 1 year prior to Day 1.
12. Be judged by the Investigator to be in good general health as documented by the medical history, physical examination (including but may not be limited to an evaluation of the cardiovascular, gastrointestinal, respiratory and central nervous systems), vital sign assessments and by general observations. Any abnormalities or deviations outside the normal ranges for any clinical testing (e.g. vital signs) can be repeated at the discretion of the Investigator and judged to be not clinically significant for study participation.

Exclusion Criteria

1. Report receiving any investigational product, participated in any previous clinical study for an investigational drug, device, biologic, or tobacco product within 30 days prior to screening.
2. Report at screening using both ENDS and conventional cigarettes (menthol or non-menthol) (dual users), or use of other inhaled products (such as, but not limited to cannabis) in the past 3 months.
3. For ENDS users, be unable to perform CPS or incapable of drawing down the pod weight by 20-60mg after 3 attempts at screening.
4. If female, be pregnant, have a positive urine pregnancy test at screening, be lactating, breast feeding, or intend to become pregnant during the time period from screening through the end of the study.
5. Have any clinically significant results from physical examinations and vital signs assessments, as judged by the Investigator.
6. Have a systolic blood pressure \< 90 mmHg or \> 150 mmHg, diastolic blood pressure \< 40 mmHg or \> 95 mmHg, or heart rate \< 40 bpm or \> 99 bpm at screening.
7. Report a clinically significant illness during the 30 days prior to enrollment, as determined by the Investigator.
8. Report a history of drug or alcohol addiction or abuse within the past 1 year.
9. Have a positive screen for alcohol or drugs of abuse at screening or at check-in on Day 1.
10. Have a body mass index (BMI) greater than 40 kg/m2 or less than 18 kg/m2 at screening.
11. Have used prescription anti-diabetic medication and/or insulin therapy within 12 months of screening.
12. Have taken medication for depression, asthma, or chronic obstructive pulmonary disease within 6 months of screening.
13. Have used prescription or over-the-counter bronchodilator medication (e.g., inhaled or oral β-agonists) within 6 months of screening.
14. Be breast-feeding or pregnant female subjects (confirmed by a positive pregnancy test). Female subjects, who are considered women of child bearing potential (WOCBP) and sexually active, must be willing and able to use an acceptable method of contraception from screening through the end of the study.
15. Have experienced an allergic reaction following previous e-cigarette use or with exposure to any primary components of the e-liquids (nicotine, flavor, benzoic acid, propylene glycol, and/or glycerol.
16. Be or have a first-degree relative (i.e. parent, sibling or child) who is a current employee of CRO, Sponsor, or site.
17. In the opinion of an Investigator, be deemed not eligible to participate in this study.
Minimum Eligible Age

21 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Juul Labs, Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Frontage Clinical Services

Secaucus, New Jersey, United States

Site Status

Rose Research Center

Charlotte, North Carolina, United States

Site Status

Rose Research Center

Raleigh, North Carolina, United States

Site Status

Countries

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United States

References

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Oldham MJ, Bailey PC, Castro N, Lang Q, Salehi A, Rostami AA. Prediction of potential passive exposure from commercial electronic nicotine delivery systems using exhaled breath analysis and computational fluid dynamic techniques. J Breath Res. 2021 Oct 5;15(4). doi: 10.1088/1752-7163/ac2884.

Reference Type DERIVED
PMID: 34544050 (View on PubMed)

Other Identifiers

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PROT-00109

Identifier Type: -

Identifier Source: org_study_id

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