Adoptive T Cell Therapy in Patients With Recurrent Ovarian Cancer

NCT ID: NCT04072263

Last Updated: 2021-04-01

Basic Information

• Recruitment Status: UNKNOWN
• Clinical Phase: PHASE1/PHASE2
• Total Enrollment: 12 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2018-08-01
• Study Completion Date: 2021-12-01

Brief Summary

The clinical benefit of standard treatment for patients with epithelial ovarian cancer (EOC) are poor. Ovarian cancer is a highly immunogenic tumor and good survival is tightly linked to the presence of tumor-infiltrating CD8+ T cells and the absence of immunosuppressive immune cells. This clear correlation between T cell infiltration and disease progression suggests that EOC may be sensitive to adoptive cell therapy by infusion of ex-vivo expanded autologous Tumor Infiltrating Lymphocytes (TIL) provided that immune suppression is reduced. Carboplatin+paclitaxel chemotherapy is directly killing tumor cells but was also shown to alleviate immunosuppression for 2 weeks coinciding with enhanced T-cell immunity, potentially creating a window of opportunity for T-cell based immunotherapy.

In addition, there is evidence that interferon alpha (IFNα) not only may work as a low toxic preconditioning regimens that creates the space required for the infused TIL but that it also supports the TIL by sustaining their persistence and indirectly their function, by upregulation of HLA class I on tumor cells and decreasing the numbers of regulatory T cells. Based on this we hypothesize that a synergistic clinical effect may be obtained when adoptive cell therapy with autologous TIL is administered during treatment with chemotherapy and IFNα. The feasibility and safety of TIL administration is studied in the window of opportunity created by carboplatin-paclitaxel chemotherapy with or without interferon alpha (IFNα). Furthermore, exploratory studies will be performed to analyze and confirm the proposed underlying mechanisms.

Tumor material for TIL production will be collected during first line debulking surgery in case of FIGO stage IIIC/IV disease (pre-OVACURE) or in case of recurrent platinum sensitive disease an extra biopsy can be planned (OVACURE).

Detailed Description

Study the feasibility and safety of TIL administration in the window of opportunity created by carboplatin-paclitaxel chemotherapy with or without interferon alpha (IFNα). Furthermore, exploratory studies will be performed to analyze and confirm the proposed underlying mechanisms.

Intervention:

Cohort 1

• Carboplatin-paclitaxel day1, q3 weeks, 6x, plus
• TIL starting 14 days after the 2nd chemotherapy cycle, q3 weeks, 3x.

Cohort 2

• Carboplatin-paclitaxel day1, q3 weeks, 6x, plus
• TIL starting 14 days after the 2nd chemotherapy cycle, q3 weeks, 3x, plus
• IFNα (3x10e6 U daily) starting one week before the first TIL infusion for 12 weeks in total.

Conditions

Ovarian Cancer Recurrent

Study Design

• Allocation Method: NON_RANDOMIZED
• Intervention Model: SEQUENTIAL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Cohort 1

• Carboplatin-paclitaxel day1, q3 weeks, 6x, plus
• Tumor Infiltrating Lymphocytes (TIL) starting 14 days after the 2nd chemotherapy cycle, q3 weeks, 3x.

Group Type: EXPERIMENTAL

Tumor Infiltrating Lymphocytes (TIL)

Intervention Type: BIOLOGICAL

Adoptive cell therapy using Tumor-Infiltrating Lymphocytes (TIL) i.v.

Carboplatin

Intervention Type: DRUG

chemotherapy i.v.

Paclitaxel

Intervention Type: DRUG

Chemotherapy i.v.

Cohort 2

• Carboplatin-paclitaxel day1, q3 weeks, 6x, plus
• Tumor Infiltrating Lymphocytes (TIL) starting 14 days after the 2nd chemotherapy cycle, q3 weeks, 3x, plus
• Interferon Alpha 2A (3x10e6 U daily) starting one week before the first TIL infusion for 12 weeks in total.

Group Type: EXPERIMENTAL

Tumor Infiltrating Lymphocytes (TIL)

Intervention Type: BIOLOGICAL

Adoptive cell therapy using Tumor-Infiltrating Lymphocytes (TIL) i.v.

Interferon Alfa 2A

Intervention Type: DRUG

Interferon alpha (IFNα) s.c., may work as a low toxic preconditioning regimens that creates the space required for the infused TIL but that it also supports the TIL by sustaining their persistence and indirectly their function, by upregulation of HLA class I on tumor cells and decreasing the numbers of regulatory T cells.

Carboplatin

Intervention Type: DRUG

chemotherapy i.v.

Paclitaxel

Intervention Type: DRUG

Chemotherapy i.v.

Interventions

Tumor Infiltrating Lymphocytes (TIL)

Adoptive cell therapy using Tumor-Infiltrating Lymphocytes (TIL) i.v.

Intervention Type: BIOLOGICAL

Interferon Alfa 2A

Interferon alpha (IFNα) s.c., may work as a low toxic preconditioning regimens that creates the space required for the infused TIL but that it also supports the TIL by sustaining their persistence and indirectly their function, by upregulation of HLA class I on tumor cells and decreasing the numbers of regulatory T cells.

Intervention Type: DRUG

Carboplatin

chemotherapy i.v.

Intervention Type: DRUG

Paclitaxel

Chemotherapy i.v.

Intervention Type: DRUG

Other Intervention Names

IFNalpha; carboplatine; Taxol

Eligibility Criteria

Inclusion Criteria

• Age ≥ 18 years.
• Histologically proven epithelial ovarian cancer (EOC).
• Recurrent ovarian cancer amenable to carboplatin and paclitaxel chemotherapy. Patient with primary stage III or IV EOC can participate in the pre-OVACURE to collect rest tumor during debulking surgery for TILs preservation, so TILs will be available in case of recurrent disease will develop in the future.
• Presence of measurable progressive disease according to RECIST version 1.1 or elevated CA125 ≥ 2 times the upper normal limit (UNL) within 3 months and confirmed.
• Expected survival of at least 3 months.
• WHO performance status 0-2.
• Within the last 2 weeks prior to study day 0, vital laboratory parameters should be within normal range, except for the following laboratory parameters, which should be within the ranges specified:

Lab Parameter Range Hemoglobin ≥ 6,0 mmol/l Granulocytes ≥ 1,500/µl Lymphocytes ≥ 700/µl Platelets ≥ 100,000/µl Creatinine clearance ≥ 50 min/ml Serum bilirubin ≤ 40 mol/l ASAT and ALAT ≤ 5 times the normal upper limit LDH ≤ 2 times the normal upper limit

• Viral tests:

• Negative for HIV type 1/2, HTLV and TPHA
• No HBV (hepatitis B virus) antigen or antibodies against HBc in the serum
• No antibodies against HCV (hepatitis C virus) in the serum
• Able and willing to give valid written informed consent.
• Prior treatment, including immunotherapy e.g. with anti-PD(L)1, is allowed but systemic therapy and radiotherapy must have been discontinued for at least two weeks before study entry.
• Patients should have disease progression.

Exclusion Criteria

• Patients with brain metastases.
• Clinically significant heart disease (NYHA Class III or IV).
• Other serious acute or chronic illnesses, e.g. active infections requiring antibiotics, bleeding disorders, or other conditions requiring concurrent medications not allowed during this study.
• Active immunodeficiency disease or autoimmune disease requiring immune suppressive drugs. Vitiligo is not an exclusion criterion.
• Other malignancy within 2 years prior to entry into the study, except for treated non-melanoma skin cancer and in situ cervical or vulva carcinoma.
• Mental impairment that may compromise the ability to give informed consent and comply with the requirements of the study.
• Lack of availability for follow-up assessments.
• Pregnancy or breastfeeding.

• Minimum Eligible Age: 18 Years
• Eligible Sex: FEMALE
• Accepts Healthy Volunteers: No

Sponsors

Leiden University Medical Center

OTHER

Sponsor Role: lead

Responsible Party

J.R. Kroep

MD PhD

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Judith Kroep, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Leiden University Medical Center

Locations

Leiden University Medical Center

Leiden, South Holland, Netherlands

Site Status: RECRUITING

Countries

Netherlands

Central Contacts

Judith Kroep, MD, PhD

Role: CONTACT

j.r.kroep@lumc.nl

0031715263464

Els Verdegaal, PhD

Role: CONTACT

E.M.E.Verdegaal@lumc.nl

0031715263464

Other Identifiers

OVACURE

Identifier Type: -

Identifier Source: org_study_id