Efficacy of Fractional CO2 Laser as a Mono- or Adjuvant Therapy for Alopecia Areata

NCT ID: NCT04003376

Last Updated: 2020-05-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE4

Total Enrollment

40 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-07-26

Study Completion Date

2022-07-01

Brief Summary

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Alopecia areata is a non-scarring hair loss disorder that affects both sexes equally. Incidence of the disease varies for different populations and in different studies, with global incidence ranging from 0.57% to 3.8%.

Etiology of alopecia areata is not completely understood, and the majority of evidence suggests that genetically predisposed individuals, when exposed to an unknown trigger, develop a predominantly autoimmune reaction, leading to acute hair loss. Environmental triggers, including viral or bacterial infections, along with autoimmune disorders, seem to play a major role in the development of alopecia areata.

Detailed Description

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The onset and progression of alopecia areata are unpredictable. Significant variations in the clinical presentation of alopecia areata have been observed, ranging from small, well-circumscribed patches of hair loss to a complete absence of body and scalp hair.

Although many patients improve spontaneously or respond to standard therapy, treatment can be quite challenging in those with more severe and refractory disease.

Recent advances in the understanding of alopecia areata pathophysiology hold promise for better treatments in the future.

Corticosteroids either topical or intralesional are the most popular drugs for the treatment of this disease. Other therapies like topical minoxidil, anthralin, immunotherapy, systemic corticosteroids, cyclosporine and Psoralen with Ultraviolet-A Light therapy are also commonly used with varying success.

Various lasers have been suggested in recent studies to treat alopecia areata . The effect of 308-nm excimer laser was the most studied, while others, including neodymium-doped yttrium aluminum garnet, erbium:glass laser, fractional carbon dioxide laser, and low-level laser therapy, have also been assessed.

Despite the nascent state of research on lasers as a treatment option for alopecia areata , there have been a great deal of promising results. It is possible for lasers to become the mainstay treatment option of alopecia areata .

It was suggested that ablative fractional lasers may exert its effect through: induction of moderate inflammation to promote anagen entry and creation of channel pathways for topically applied medications they grant access to dermal structures such as hair follicles and cutaneous vasculature. Also, creation of a wound by the ablative fractional laser may stimulate stem cell populations to produce a hair shaft and progress through all stages of the hair follicle cycle.

Majid et al, investigated the efficacy and safety of the combination of fractional carbon dioxide followed by topical triamcinolone acetonide application in ten patients with resistant alopecia areata. Only eight patients completed the study. Seven of them had complete recovery of the treated area. One patient however did not show good response. No significant adverse effects were noted in any of the patients.

The role of platelet rich plasma in promoting hair survival and growth has been demonstrated both in vitro and in vivo. The activation of platelet α granules releases numerous growth factors, including transforming growth factor , platelet derived growth factor, vascular endothelial growth factor, epidermal growth factor, insulin-like growth factor, and interleukin-1. It is proposed that these growth factors may act on stem cells in the bulge area of the follicles, stimulating the development of new follicles and promoting neovascularization. platelet rich plasma has been found to benefit in hair growth in alopecia areata. Intralesional injections of platelet rich plasma were found to increase hair regrowth significantly compared with triamcinolone acetonide or placebo.

Vitamin D 1, 25-dihydroxycholecalciferol \[1, 25(OH)/2 D3\] is the biologic active form of the vitamin D3. Vitamin D has a multitude of biologic effects interacting with the innate and adaptive immune system, mainly leading to its downregulation. It regulates the differentiation of B cells, T cells, dendritic cells, and the expression of Toll-like receptors. There is growing evidence that vitamin D may help in several autoimmune diseases like multiple sclerosis and type I diabetes mellitus, lupus, and rheumatoid arthritis.

The relation between vitamin D levels and the development of alopecia areata and whether vitamin D supplementation helps in the treatment of alopecia areata represent an attractive area of research. Recent studies suggest that there is deficiency of serum vitamin D and reduced vitamin D receptor expression in the affected hair follicles in alopecia areata patients, the results of which may prove that vitamin D is a safe and helpful choice in alopecia areata treatment.

To the best our knowledge no previous research studied the effect of vitamin D solution in treatment of alopecia areata.

Conditions

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Alopecia Areata

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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fractional carbon dioxide laser alone

six sessions of fractional carbon dioxide laser will be done for 10 patients with alopecia areata

Group Type ACTIVE_COMPARATOR

fractional carbon dioxide laser alone

Intervention Type DEVICE

six sessions of fractional carbon dioxide laser will be done for alopecia areata lesions

fractional carbon dioxide laser and triamcinolone acetonide

six sessions of fractional carbon dioxide laser will be done for 10 patients with alopecia areata followed immediately by topical application of triamcinolone acetonide (10mg/ml)

Group Type EXPERIMENTAL

fractional carbon dioxide laser alone

Intervention Type DEVICE

six sessions of fractional carbon dioxide laser will be done for alopecia areata lesions

Triamcinolone Acetonide 10mg/mL

Intervention Type DRUG

six sessions of fractional carbon dioxide laser will be done for 10 patients with alopecia areata followed immediately by topical application of triamcinolone acetonide

fractional carbon dioxide laser and platelet rich plasma

six sessions of fractional carbon dioxide laser will be done for 10 patients with alopecia areata followed immediately by topical application of autologous platelet rich plasma

Group Type EXPERIMENTAL

fractional carbon dioxide laser alone

Intervention Type DEVICE

six sessions of fractional carbon dioxide laser will be done for alopecia areata lesions

platelet rich plasma

Intervention Type BIOLOGICAL

six sessions of fractional carbon dioxide laser will be done for 10 patients with alopecia areata followed immediately by topical application of autologous platelet rich plasma

fractional carbon dioxide laser and vitamin D solution

six sessions of fractional carbon dioxide laser will be done for 10 patients with alopecia areata followed immediately by topical application of vitamin D solution

Group Type EXPERIMENTAL

fractional carbon dioxide laser alone

Intervention Type DEVICE

six sessions of fractional carbon dioxide laser will be done for alopecia areata lesions

vitamin D solution

Intervention Type DRUG

six sessions of fractional carbon dioxide laser will be done for 10 patients with alopecia areata followed immediately by topical application of vitamin D solution

Interventions

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fractional carbon dioxide laser alone

six sessions of fractional carbon dioxide laser will be done for alopecia areata lesions

Intervention Type DEVICE

Triamcinolone Acetonide 10mg/mL

six sessions of fractional carbon dioxide laser will be done for 10 patients with alopecia areata followed immediately by topical application of triamcinolone acetonide

Intervention Type DRUG

platelet rich plasma

six sessions of fractional carbon dioxide laser will be done for 10 patients with alopecia areata followed immediately by topical application of autologous platelet rich plasma

Intervention Type BIOLOGICAL

vitamin D solution

six sessions of fractional carbon dioxide laser will be done for 10 patients with alopecia areata followed immediately by topical application of vitamin D solution

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* patients with refractory alopecia areata above 10 years old

Exclusion Criteria

* Alopecia totalis or alopecia universalis.
* Patients with alopecia other than alopecia areata.
* Patients younger than 10 years.
* Pregnant and lactating females.
* Patients with history of hypertrophic scars or keloid formation.
* Patients with active infection at the site of the lesion.
* Patients with blood disorders and platelet abnormalities and chronic liver disease.
* Patients using anticoagulation therapy and antiplatelet agents.
Minimum Eligible Age

10 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Assiut University

OTHER

Sponsor Role lead

Responsible Party

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A Ghazally

principal investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Alaa Ghazally, MS

Role: PRINCIPAL_INVESTIGATOR

Assiut University

Locations

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Assiut University Hospital

Asyut, , Egypt

Site Status RECRUITING

Countries

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Egypt

Central Contacts

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Azza Mahfouz, PHD

Role: CONTACT

01001801039

Radwa Bakr, PHD

Role: CONTACT

01119988115

Facility Contacts

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Alaa Ghazally, MS

Role: primary

01007224787

References

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Aksu Cerman A, Sarikaya Solak S, Kivanc Altunay I. Vitamin D deficiency in alopecia areata. Br J Dermatol. 2014 Jun;170(6):1299-304. doi: 10.1111/bjd.12980.

Reference Type BACKGROUND
PMID: 24655364 (View on PubMed)

Alkhalifah A, Alsantali A, Wang E, McElwee KJ, Shapiro J. Alopecia areata update: part II. Treatment. J Am Acad Dermatol. 2010 Feb;62(2):191-202, quiz 203-4. doi: 10.1016/j.jaad.2009.10.031.

Reference Type BACKGROUND
PMID: 20115946 (View on PubMed)

Huang Y, Zhuo F, Li L. Enhancing hair growth in male androgenetic alopecia by a combination of fractional CO2 laser therapy and hair growth factors. Lasers Med Sci. 2017 Nov;32(8):1711-1718. doi: 10.1007/s10103-017-2232-8. Epub 2017 May 21.

Reference Type BACKGROUND
PMID: 28528395 (View on PubMed)

Issa MC, Pires M, Silveira P, Xavier de Brito E, Sasajima C. Transepidermal drug delivery: a new treatment option for areata alopecia? J Cosmet Laser Ther. 2015 Feb;17(1):37-40. doi: 10.3109/14764172.2014.967778. Epub 2014 Oct 16.

Reference Type BACKGROUND
PMID: 25260052 (View on PubMed)

Other Identifiers

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CO2LASERAA

Identifier Type: -

Identifier Source: org_study_id

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