Evaluation of a Donor Testing Kit for the Prediction of AGVHD in Patient Receiving a Peripheral Blood Stem Cell Allograft

NCT ID: NCT03887156

Last Updated: 2021-09-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE2/PHASE3

Total Enrollment

227 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-09-16

Study Completion Date

2023-03-16

Brief Summary

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The aim is to validate an in vitro diagnosis medical device to predict grade II to IV aGVHD after a cell graft

Detailed Description

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Acute Graft Versus Host Disease (aGVHD) is the most frequent complication in allogeneic Hematopoietic Stem Cell Transplantation (allo-HSCT). It affects up to 50% patients, among whom 15% to 25% develop severe forms, often lethal, yet impossible to predict even for donors with a Human Leukocyte Antigene (HLA) 10/10 compatibility. Global Overall Survival (OS) after transplantation is 40% to 60% only due to post transplant severe complications. There is a major medical need for a technology that would predict the risk of aGVHD and would allow the selection of a favourable donor among multiple Human Leukocyte Antigene (HLA)10/10 compatible donors.

MT. Rubio and M. Bouillié at Pr Olivier Hermine's lab previously reported that enhanced early post-transplant invariant Natural Killer T (iNKT) cells reconstitution from donor cells was correlated to reduced risk of aGVHD, without impairment of the Graft Versus Leukemia (GVL) effect. They subsequently demonstrated that the expansion of donors CD4neg invariant Natural Killer T (iNKT) cells subpopulation was predictive of a reduced risk of aGVHD, and developed a method for predicting this risk based on the expansion factor of CD4neg invariant Natural Killer T (iNKT) cells in the peripheral blood stem cell (PBSC) graft. This invariant Natural Killer T (iNKT) cells functional test reaches its optimal predictive capacity with 94% sensitivity and 100% specificity in allo-HSCT performed with Human Leukocyte Antigene (HLA) 10/10 matched peripheral blood stem cell (PBSC) grafts for non-progressive hematological malignant diseases, in complete response, which represent the majority of the indications of allogeneic HSCT. Similar predictive value was also observed when the test was performed from donor's peripheral blood before G-CSF mobilization. It was not associated with an increased risk of relapse. This test could therefore allow to easily selecting the best donor if different siblings or unrelated donors are available before PBSC allo-HSCT.

Conditions

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Acute Myeloid Leukemia in Remission Lymphoma in Remission Non-progressive Myeloproliferative Syndrome Myelodysplasia With Stable Blasts is Cell Number and < 10% of Blastocysts Acute Leukemia Biphenotypic in Remission Acute Lymphoblastic Leukemia

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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Arm 1

One arm

Group Type OTHER

Ex vivo capacities of CD4neg INkT expansion of the peripheral blood donor with the Predictor test

Intervention Type DIAGNOSTIC_TEST

Calculation of ex vivo capacities of CD4neg INkT expansion of the peripheral blood from an identified donor for an allograft. Sample is collected before mobilization and the blood culture and analysis using the Predictor test are performed by the central lab.

Interventions

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Ex vivo capacities of CD4neg INkT expansion of the peripheral blood donor with the Predictor test

Calculation of ex vivo capacities of CD4neg INkT expansion of the peripheral blood from an identified donor for an allograft. Sample is collected before mobilization and the blood culture and analysis using the Predictor test are performed by the central lab.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* PATIENT :

* Age between 18 and 65 years ( included )
* Being candidate to a graft of peripheral hematopoietic stem cells , according the following criteria :
* HLA compatibility 10 / 10 with the selected donor
* Malignant haematological disorder as described below :

* Acute Myeloid Leukemia (AML) or Acute Lymphoblastic Leukemia ( ALL) in 1st or 2d complete remission
* Aggressive lymphoma in complete remission
* Non - progressive myeloproliferative syndrome ,
* Myelodysplasia with stable blasts is cell number and \< 10 % of blastocysts,
* Acute leukemia biphenotypic in 1st or 2d complete remission
* Sequential graft conditioning, myeloablative or with a reduced intensity, both may include ATG
* Classical scheme for immunosuppression decrease ( from day 90 to day 180 ) • Not being opposed to medical data collection DONOR
* Adult ( ≥ 18 year old) up to the maximum authorized by each National Transplantation Authority
* Being a patient's sibling or registered in the Bone Marrow Donors Worldwide registry or a national registry
* Being candidate to a Peripheral Blood Stem Cells donation with a Human Leucocyt Antigen (HLA) 10 / 10 compatibility with the recipient ,
* Signed and dated informed consent ( in accordance with local regulation of the country in which the observation is performed )

Exclusion Criteria

* Participating in a clinical trial, if interventional on the prophylaxis treatment ( not on the prophylaxis ) of GVHD, in the 30 days prior to the inclusion and during the Predictor 2 study ,
* Being placed under legal supervision ,
* Presenting any impossibility to fulfil the study requirements, due to geographical, social or physical reasons
Minimum Eligible Age

18 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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SATT

UNKNOWN

Sponsor Role collaborator

SNC Graft Versus Host Disease

UNKNOWN

Sponsor Role collaborator

Imagine Institute

OTHER

Sponsor Role collaborator

Axonal-Biostatem

INDUSTRY

Sponsor Role collaborator

CERBA laboratory

UNKNOWN

Sponsor Role collaborator

Assistance Publique - Hôpitaux de Paris

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Olivier Hermine, MD

Role: PRINCIPAL_INVESTIGATOR

Head of adult hematology department

Locations

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Z.N.A. Stuivenberg Ziekenhuis

Antwerp, , Belgium

Site Status RECRUITING

CHU Liège

Liège, , Belgium

Site Status RECRUITING

U.Z. Antwerpen

Wilrijk, , Belgium

Site Status RECRUITING

CHU Amiens-Picardie

Amiens, , France

Site Status RECRUITING

CHU Angers

Angers, , France

Site Status RECRUITING

CHU de Caen

Caen, , France

Site Status RECRUITING

HIA Percy

Clamart, , France

Site Status RECRUITING

CHU Clermont-Ferrand

Clermont-Ferrand, , France

Site Status RECRUITING

Hôpital Dupuyten

Limoges, , France

Site Status RECRUITING

Hôtel Dieu

Nantes, , France

Site Status RECRUITING

CHU Nice

Nice, , France

Site Status RECRUITING

Hôpital de la Pitiè-Salpétrière

Paris, , France

Site Status RECRUITING

Hôpital Necker Enfants Malades

Paris, , France

Site Status RECRUITING

CHU Bordeaux

Pessac, , France

Site Status RECRUITING

CHU de Poitiers

Poitiers, , France

Site Status RECRUITING

CHU de Rennes

Rennes, , France

Site Status RECRUITING

L'Institut de Cancérologie de la Loire

Saint-Priest-en-Jarez, , France

Site Status RECRUITING

Institut Universitaire du Cancer de Toulouse

Toulouse, , France

Site Status RECRUITING

CHRU Nancy - Hôpital de Brabois

Vandœuvre-lès-Nancy, , France

Site Status RECRUITING

Donor Site - Koln

Cologne, , Germany

Site Status ACTIVE_NOT_RECRUITING

Donor Site-Dresden

Dresden, , Germany

Site Status ACTIVE_NOT_RECRUITING

Medizinische Hochschule Hannover

Hanover, , Germany

Site Status RECRUITING

Countries

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Belgium France Germany

Central Contacts

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Yannick VACHER

Role: CONTACT

+33 1 44 84 17 30

Marie-Lou CAMUT

Role: CONTACT

+33 1 56 38 21 77

Facility Contacts

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Dimitri BREEMS

Role: primary

0032 3 217 73 97

Yves BEGUIN, Pr

Role: primary

003243667201

Anke VERLINDEN, Dr

Role: primary

0032 478 27 86 24

Magalie JORIS, Dr

Role: primary

03 22 45 59 20

Sylvie FRANCOIS, Dr

Role: primary

02.41.35.44.75

Hyacinthe Atchroué JOHNSON-ANSAH, Dr

Role: primary

02 31 27 20 20

Jean-Valère MALFUSON, Pr

Role: primary

01.41.46.63.07

Lemal RICHARD, Dr

Role: primary

04 73 75 00 65

Arnaud JACCARD, Dr

Role: primary

05 55 05 66 52

Patrice CHEVALLIER, Dr

Role: primary

02.40.08.32.69

Pierre-Simon ROHRLICH, Dr

Role: primary

04 92 03 58 41

Stéphanie NGUYEN-QUOC, Pr

Role: primary

01 42 16 28 25

Olivier HERMINE, Pr

Role: primary

01.44.49.52.82

Carmen BOTELLA GARCIA, Dr

Role: primary

05 57 65 65 11

Natacha MAILLARD, Dr

Role: primary

05.49.44.43.07

Marc BERNARD, Dr

Role: primary

02 99 28 43 21

Emilie CHALAYER, Dr

Role: primary

04 77 91 70 60

Anne HUYNH, Dr

Role: primary

05 31 15 63 54

Marie-Thérèse RUBIO

Role: primary

03 83 15 32 82

Michael STADLER

Role: primary

References

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Rubio MT, Bouillie M, Bouazza N, Coman T, Trebeden-Negre H, Gomez A, Suarez F, Sibon D, Brignier A, Paubelle E, Nguyen-Khoc S, Cavazzana M, Lantz O, Mohty M, Urien S, Hermine O. Pre-transplant donor CD4- invariant NKT cell expansion capacity predicts the occurrence of acute graft-versus-host disease. Leukemia. 2017 Apr;31(4):903-912. doi: 10.1038/leu.2016.281. Epub 2016 Oct 14.

Reference Type BACKGROUND
PMID: 27740636 (View on PubMed)

Other Identifiers

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K180304J

Identifier Type: -

Identifier Source: org_study_id

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