Clinical Trial to Evaluate the Safety, Tolerability, Pharmacokinetics and Pharmacodynamics of Tuspetinib (HM43239) in Patients With Relapsed or Refractory Acute Myeloid Leukemia

NCT ID: NCT03850574

Last Updated: 2025-08-26

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE1/PHASE2

Total Enrollment

240 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-03-11

Study Completion Date

2027-04-30

Brief Summary

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The main purpose of this study is to identify a safe and potentially effective dose of tuspetinib to be used in future studies in study participants diagnosed with acute myeloid leukemia (AML), myelodysplastic syndromes with increased blasts grade 2 (MDS-IB2), or chronic myelomonocytic leukemia (CMML) that is relapsed or refractory after at least one line of prior therapy, or in study participants with newly diagnosed AML. Tuspetinib will be administered as a single agent or in combination with other drugs (venetoclax or venetoclax plus azacitidine), as specified for each part of the study.

Detailed Description

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This is a Phase 1/2, open-label, multi-center study designed to assess the efficacy, safety, tolerability, and pharmacokinetics, including determining the recommended Phase 2 dose (RP2D) of tuspetinib (HM43239) in subjects with relapsed or treatment-refractory acute myeloid leukemia (AML).

Part C: This portion of the study will evaluate tuspetinib (HM43239) as monotherapy in patients with relapsed or refractory (R/R) AML, focusing on safety, tolerability, pharmacokinetics, and preliminary efficacy (Aptivate).

Part D: This portion of the study will evaluate the safety, tolerability, and PK parameters of tuspetinib (HM43239) in combination with venetoclax and azacitidine when administered to newly diagnosed AML patients who are ineligible for induction chemotherapy (Tuscany).

Conditions

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Leukemia, Myeloid, Acute Refractory AML Relapsed Adult AML Myelodysplastic Syndrome With Excess Blasts-2 Chronic Myelomonocytic Leukemia

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SEQUENTIAL

1. Part A: Tuspetinib dose escalation
2. Part B: Tuspetinib dose exploration
3. Part C: Tuspetinib monotherapy dose expansion
4. Part C: Tuspetinib in combination with venetoclax ("doublet" therapy) dose expansion
5. Part D: Tuspetinib in combination with venetoclax and azacitidine ("triplet" therapy) dose exploration
Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Part A Dose Escalation [COMPLETED]

Part A dose escalation of tuspetinib as a single agent is planned for up to 6 dose levels. If a study participant in dose escalation at any dose level achieves clinical response, then the dose level will continue to enroll in Part B. If one DLT or less is observed in the 6 participants (\<1/6 DLT observed) in Part A, up to 20 evaluable participants can be enrolled in Part B at that dose level.

Group Type EXPERIMENTAL

Tuspetinib

Intervention Type DRUG

Daily (QD), continuous dosing

Part B Dose Exploration [ACTIVE, NOT RECRUITING]

Part B dose exploration of tuspetinib as a single agent is planned for up to 4 dose levels.

Group Type EXPERIMENTAL

Tuspetinib

Intervention Type DRUG

Daily (QD), continuous dosing

Part C Dose Expansion (tuspetinib as a single agent) [COMPLETE]

Part C dose expansion of tuspetinib as a single agent is planned for 2 dose levels. Study participants will be randomly assigned to either arm based on the number of slots available. The initial tuspetinib dose will be 120 mg.

Group Type EXPERIMENTAL

Tuspetinib

Intervention Type DRUG

Daily (QD), continuous dosing

Part C Dose Expansion (tuspetinib plus venetoclax) [COMPLETE]

Part C dose expansion of tuspetinib in combination with venetoclax is planned for 2 dose levels. The initial tuspetinib dose will be 80 mg.

Group Type EXPERIMENTAL

Tuspetinib

Intervention Type DRUG

Daily (QD), continuous dosing

Venetoclax Oral Tablet

Intervention Type DRUG

Venetoclax will be given to study participants in the Part C tuspetinib plus venetoclax combination treatment group either in 50 mg or 100 mg tablets

Part D Dose Exploration (tuspetinib plus venetoclax and azacitidine) [ACTIVE, RECRUITING - US Sites]

Part D dose exploration of tuspetinib in combination with venetoclax and azacitidine is planned for up to 6 dose levels. The initial tuspetinib dose will be 40 mg.

Group Type EXPERIMENTAL

Tuspetinib

Intervention Type DRUG

Daily (QD), continuous dosing

Venetoclax Oral Tablet

Intervention Type DRUG

Venetoclax will be given to study participants in the Part C tuspetinib plus venetoclax combination treatment group either in 50 mg or 100 mg tablets

Azacitidine for Intravenous Infusion

Intervention Type DRUG

Azacitidine will be given to study participants in Part D as intravenous infusion at a dose of 75 mg/m\^2

Interventions

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Tuspetinib

Daily (QD), continuous dosing

Intervention Type DRUG

Venetoclax Oral Tablet

Venetoclax will be given to study participants in the Part C tuspetinib plus venetoclax combination treatment group either in 50 mg or 100 mg tablets

Intervention Type DRUG

Azacitidine for Intravenous Infusion

Azacitidine will be given to study participants in Part D as intravenous infusion at a dose of 75 mg/m\^2

Intervention Type DRUG

Other Intervention Names

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HM43239 Venetoclax Azacitidine

Eligibility Criteria

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Inclusion Criteria

* Study participant is defined as having morphologically documented primary or secondary AML, MDS-IB2 (≥ 10% bone marrow blasts), or CMML by the World Health Organization (WHO) criteria (2016), and fulfills one of the following:

1. Refractory to at least 1 cycle of prior therapy
2. Relapsed after achieving remission with a prior therapy
* Study participant has an Eastern Cooperative Oncology Group (ECOG) performance status ≤ 2.
* Study participant's interval from prior treatment to time of study drug administration is at least 2 weeks for cytotoxic agents (except hydroxyurea given for controlling blast cells), at 4 weeks for biologic or cellular immunotherapies, or least 5 half-lives for prior experimental agents or noncytotoxic agents, including immunosuppressive therapy post hematopoietic stem cell transplantation (HSCT). Upon discussion with the Medical Monitor, a shorter than stated washout period may be considered provided that the study participant has recovered from any clinically relevant safety issue and recovered to Grade ≤ 1 toxicity from prior therapies.
* Study participant must meet the following criteria as indicated on the clinical laboratory tests.

1. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) ≤ 3× institutional upper limit normal (ULN)
2. Total serum bilirubin ≤ 1.5× institutional ULN
3. Creatinine clearance as calculated by the Cockcroft-Gault formula or measured by 24-h urine collection of \> 45 mL/min.
* Study participant is suitable for oral administration of study drug and has minimum life expectancy (≥ 3 months)
* Female study participants must be either:
* Of non-childbearing potential

1. Post-menopausal (defined as at least 1 year without any menses) prior to screening, or
2. Documented surgically sterile or status post hysterectomy (at least 1 month prior to screening)
* Or, if of childbearing potential,

1. Must have a negative serum or urine pregnancy test at screening (within 72 hours prior to start of treatment), and
2. Must use an acceptable highly effective method of birth control starting at screening and throughout the study period and for 90 days after the final study drug administration.
* Female study participants must not be breastfeeding at screening and during the study period, and for 90 days after the final study drug administration
* Female study participants must not donate ova starting at screening and throughout the study period, and for 90 days after the final study drug administration.
* Male study participants and their female spouse/partners who are of childbearing potential must be using highly effective contraception starting at screening and continue throughout the study period and for 90 days after the final study drug administration.
* Male study participants must not donate sperm starting at screening and throughout the study period and for 90 days after the final study drug administration.
* Study participant agrees not to participate in another interventional study while on treatment.


* Study participant is defined as having morphologically documented newly diagnosed previously untreated primary or secondary AML by the World Health Organization (WHO) criteria (2016) and considered ineligible to receive intensive chemotherapy.

Study participants ≥ 75 years of age are considered ineligible to receive intensive chemotherapy if they meet any of the following criteria:

1. ECOG Performance Status of 2 or 3;
2. Cardiac history of congestive heart failure (CHF) requiring treatment or ejection fraction ≤ 50% or chronic stable angina;
3. Diffusing capacity of the lung for carbon monoxide (DLCO) ≤ 65% or forced expiratory volume during the first second (FEV1) ≤ 65%;
4. Creatinine clearance ≥ 30 mL/min to \< 45 ml/min;
5. Moderate hepatic impairment with total bilirubin \> 1.5 to ≤ 3.0×ULN;
6. Any other comorbidity that the Investigator judges to be incompatible with intensive chemotherapy must be reviewed and approved by the Medical Monitor during screening and prior to treatment.

Study participants are considered ineligible to receive intensive chemotherapy based on their age being ≥ 75 years.

* Study participant \< 75 years has an ECOG performance status ≤ 2; study participant ≥ 75 years has an ECOG performance status 0-2.
* Study participant must meet the following criteria as indicated on the clinical laboratory tests:

1. Serum AST and ALT ≤ 3× institutional ULN unless considered due to leukemic organ involvement.
2. Total serum bilirubin ≤ 3x institutional ULN unless considered due to leukemic organ involvement for study participants \< 75 years; total serum bilirubin ≤ 1.5x institutional ULN (or ≤ 3x institutional ULN if documented history of Gilbert's syndrome) unless considered due to leukemic organ involvement for study participants ≥ 75 years.
3. Creatinine clearance as calculated by the Cockcroft-Gault formula or measured by 24-h urine collection of ≥ 30 mL/min for study participants \< 75 years; creatinine clearance as calculated by the Cockcroft-Gault formula or measured by 24-h urine collection of ≥ 45 mL/min for study participants ≥ 75 years.
* Study participant is suitable for oral administration of study drug and has minimum life expectancy (≥ 3 months)
* Female study participants must be either:
* Of non-childbearing potential

1. Post-menopausal (defined as at least 1 year without any menses) prior to screening, or
2. Documented surgically sterile or status post hysterectomy (at least 1 month prior to screening)
* Or, if of childbearing potential,

1. Must have a negative serum or urine pregnancy test at screening (within 72 hours prior to start of treatment), and
2. Must use an acceptable highly effective contraception starting at screening and throughout the study period and for 90 days after the final study drug administration.
* Female study participants must not be breastfeeding at screening and during the study period, and for 90 days after the final study drug administration.
* Female study participants must not donate ova starting at screening and throughout the study period, and for 90 days after the final study drug administration.
* Male study participants and their female spouse/partners who are of childbearing potential must be using highly effective contraception starting at screening and continue throughout the study period and for 90 days after the final study drug administration.
* Male study participants must not donate sperm starting at screening and throughout the study period and for 90 days after the final study drug administration.
* Study participant agrees not to participate in another interventional study while on treatment.

Exclusion Criteria

* Study participant was diagnosed with acute promyelocytic leukemia (APL).
* Study participant has known BCR-ABL-positive leukemia.
* Study participant has an active malignancy other than AML, MDS-IB2, or CMML.
* Study participant has persistent non-hematological toxicities of ≥ Grade 2 (CTCAE v4.03), with symptoms and objective findings, from prior AML, MDS-IB2, or CMML treatment (including chemotherapy, kinase inhibitors, immunotherapy, experimental agents, radiation, or surgery)
* Study participant has had hematopoietic stem cell transplant (HSCT) and meets any of the following criteria:

1. Has undergone HSCT within the 2-month period prior to the first study dose
2. Has clinically significant graft-versus-host-disease (GVHD) requiring treatment
3. Has ≥ Grade 2 persistent non-hematological toxicity related to the transplant
4. Had a donor lymphocyte infusion (DLI) ≤ 30 days prior to the first study dose.
* Study participant has meningeal or central nervous system (CNS) involvement with leukemia or other CNS disease related to underlying and secondary effects of malignancy.
* Study participant has disseminated intravascular coagulation abnormality (DIC).
* Study participant has had major surgery within 4 weeks prior to the first study dose.
* Study participant has had radiation therapy within 4 weeks prior to the first study dose.
* Study participant has congestive heart failure New York Heart Association (NYHA) class 3 or 4, or study participant with a history of congestive heart failure NYHA class 3 or 4 in the past, unless a screening echocardiogram or multigated acquisition (MUGA) scan performed within 3 months prior to study entry results in a left ventricular ejection fraction (LVEF) that is ≥ 45%.
* Study participant has any of the following cardiac abnormalities of history:

1. Study participant has any clinically important abnormalities in rhythm, conduction or morphology of resting ECG, e.g., complete left bundle branch block, third-degree heart block, second-degree heart block, or PR interval \> 250 milliseconds (ms).
2. Study participant has a mean QT interval (QTc) by Friderica's method (QTcF) \> 450 ms in three successive screening measurements.
3. Study participant has any factors that increase the risk of QTc prolongation or risk of arrhythmic events, such as congenital long QT, syndrome, family history of long QT syndrome.
* Study participant is known to have active infection including any identified active COVID-19 infection.
* Study participant is known to have human immunodeficiency virus infection.
* Study participant has known active hepatitis B or C, or other active hepatic disorder.
* Study participant has any condition which, in the Investigator's opinion, makes the study participant unsuitable for study participation.
* Study participant has a history of Grade 3 or 4 non-hematologic toxicity related to tyrosine kinase inhibitor.



* Study participant was diagnosed with acute promyelocytic leukemia (APL).
* Study participant has known BCR-ABL-positive leukemia.
* Study participant has an active malignancy other than AML.
* Study participant has received treatment with the following:

1. An HMA, VEN (or other BCL-2 inhibitor), a tyrosine kinase inhibitor (TKI), a FLT3 inhibitor (FLT3i), a hematopoietic stem cell transplant (HSCT), and/or a chemotherapeutic agent for antecedent myeloid neoplasm (Note: Prior chemotherapy for solid tumors considered in remission is allowed.)
2. CAR-T cell therapy
3. Experimental therapies for antecedent myeloid neoplasms
4. Current participation in another research or observational study
* Study participant has persistent non-hematological toxicities of ≥ Grade 2 (CTCAE v4.03), with symptoms and objective findings, from treatment for antecedent myeloid neoplasms (including chemotherapy, kinase inhibitors, immunotherapy, experimental agents, immunosuppressive therapy, radiation, or surgery).
* Study participant has meningeal or central nervous system (CNS) involvement with leukemia or other CNS disease related to underlying and secondary effects of malignancy.
* Study participant has disseminated intravascular coagulation abnormality (DIC).
* Study participant has had major surgery within 4 weeks prior to the first study dose.
* Study participant has had radiation therapy within 4 weeks prior to the first study dose.
* Study participant has congestive heart failure New York Heart Association (NYHA) class 3 or 4, or study participant with a history of congestive heart failure NYHA class 3 or 4 in the past, unless a screening echocardiogram or multigated acquisition (MUGA) scan performed within 3 months prior to study entry results in a left ventricular ejection fraction (LVEF) that is ≥ 45%.
* Study participant has any of the following cardiac abnormalities of history:

1. Study participant has any clinically important abnormalities in rhythm, conduction or morphology of resting ECG, e.g., complete left bundle branch block, third-degree heart block, second-degree heart block, or PR interval \> 250 milliseconds (ms).
2. Study participant has a mean QT interval (QTc) by Friderica's method (QTcF) \> 450 ms in three successive screening measurements.
3. Study participant has any factors that increase the risk of QTc prolongation or risk of arrhythmic events, such as congenital long QT, syndrome, family history of long QT syndrome.
* Study participant is known to have active infection, including any identified active COVID-19 infection.
* Study participant is known to have human immunodeficiency virus infection.
* Study participant has known active hepatitis B or C, or other active hepatic disorder.
* Study participant has any condition which, in the Investigator's opinion, makes the study participant unsuitable for study participation, including a chronic respiratory disease that requires continuous oxygen, or a significant history of renal, neurologic, psychiatric, endocrinologic, metabolic, immunologic, hepatic, or cardiovascular disease, or any other medical condition or known hypersensitivity to any of the study medications including excipients of VEN and AZA that in the opinion of the Investigator would adversely affect participating in this study.
* Study participant exhibits evidence of other clinically significant uncontrolled systemic infection requiring therapy (viral, bacterial, or fungal) or other medical conditions (e.g., infection, heart failure, COPD flare, etc.).
* Study participant has a white blood cell count \> 25 × 10\^9/L. (Treatment with hydroxyurea or use of leukapheresis are permitted to meet this criterion.)
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Aptose Biosciences Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Naval Daver, MD

Role: PRINCIPAL_INVESTIGATOR

M.D. Anderson Cancer Center

Locations

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The Kirklin Clinic of UAB Hospital

Birmingham, Alabama, United States

Site Status RECRUITING

City of Hope Comprehensive Cancer Center

Duarte, California, United States

Site Status ACTIVE_NOT_RECRUITING

University of California Irvine

Irvine, California, United States

Site Status RECRUITING

UCSD Moores Cancer Center

La Jolla, California, United States

Site Status ACTIVE_NOT_RECRUITING

USC/Norris Comprehensive Cancer Center

Los Angeles, California, United States

Site Status RECRUITING

Stanford Cancer Center

Palo Alto, California, United States

Site Status RECRUITING

University of California, Davis

Sacramento, California, United States

Site Status RECRUITING

Yale University

New Haven, Connecticut, United States

Site Status RECRUITING

University of Miami - Miller School of Medicine

Miami, Florida, United States

Site Status RECRUITING

Emory University

Atlanta, Georgia, United States

Site Status ACTIVE_NOT_RECRUITING

Massachusetts General Hospital

Boston, Massachusetts, United States

Site Status ACTIVE_NOT_RECRUITING

Duke University Medical Center

Durham, North Carolina, United States

Site Status RECRUITING

Cleveland Clinic - Taussig Cancer Center

Cleveland, Ohio, United States

Site Status ACTIVE_NOT_RECRUITING

The Ohio State University Wexner Medical Center

Columbus, Ohio, United States

Site Status RECRUITING

MD Anderson Cancer Center

Huston, Texas, United States

Site Status RECRUITING

Border Medical Oncology

Albury, New South Wales, Australia

Site Status ACTIVE_NOT_RECRUITING

Royal Brisbane and Women's Hospital

Herston, Queensland, Australia

Site Status ACTIVE_NOT_RECRUITING

Townsville University Hospital

Townsville, Queensland, Australia

Site Status ACTIVE_NOT_RECRUITING

St Vincent's Hospital Melbourne

Fitzroy, Victoria, Australia

Site Status ACTIVE_NOT_RECRUITING

Sir Charles Gairdner Hospital

Nedlands, Western Australia, Australia

Site Status ACTIVE_NOT_RECRUITING

Universitätsklinikum Leipzig

Leipzig, Saxony, Germany

Site Status ACTIVE_NOT_RECRUITING

Charité Universitätsmedizin Berlin

Berlin, State of Berlin, Germany

Site Status ACTIVE_NOT_RECRUITING

Auckland City Hospital

Grafton, Auckland, New Zealand

Site Status ACTIVE_NOT_RECRUITING

Seoul National University Hospital

Seoul, Seoul, South Korea

Site Status COMPLETED

Asan Medical Center

Seoul, Seoul, South Korea

Site Status ACTIVE_NOT_RECRUITING

Samsung Medical Center

Seoul, Seoul, South Korea

Site Status ACTIVE_NOT_RECRUITING

Kyungpook National University Hospital

Daegu, , South Korea

Site Status ACTIVE_NOT_RECRUITING

Pusan National University Hospital

Pusan, , South Korea

Site Status ACTIVE_NOT_RECRUITING

Seoul National University Bundang Hospital

Seongnam, , South Korea

Site Status ACTIVE_NOT_RECRUITING

Hospital Universitario Vall d'Hebron

Barcelona, Barcelona, Spain

Site Status ACTIVE_NOT_RECRUITING

Hospital Quirón Madrid

Pozuelo de Alarcón, Madrid, Spain

Site Status ACTIVE_NOT_RECRUITING

Hospital Universitario Central de Asturias

Oviedo, Principality of Asturias, Spain

Site Status ACTIVE_NOT_RECRUITING

Hospital Clinico Universitario de Valencia

Valencia, Valencia, Spain

Site Status ACTIVE_NOT_RECRUITING

Hospital Universitari i Politècnic La Fe

Valencia, Valencia, Spain

Site Status ACTIVE_NOT_RECRUITING

Countries

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United States Australia Germany New Zealand South Korea Spain

Central Contacts

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Rafael Bejar, MD, PhD

Role: CONTACT

858-401-6852

Facility Contacts

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Pankit Vachhani, MD

Role: primary

Deepa Jeyakumar, MD

Role: primary

Eric Tam, MD

Role: primary

Gabriel Mannis, MD

Role: primary

Brian Jonas

Role: primary

(916) 703-9151

Nikolai Podoltsev, MD

Role: primary

Justin Watts, MD

Role: primary

Harry Erba, MD

Role: primary

Uma Borate, MD

Role: primary

Naval Daver, Dr

Role: primary

713-792-6477

References

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Sonowal H, Rice WG, Bejar R, Byun JY, Jung SH, Sinha R, Howell SB. Preclinical Development of Tuspetinib for the Treatment of Acute Myeloid Leukemia. Cancer Res Commun. 2025 Jan 1;5(1):74-83. doi: 10.1158/2767-9764.CRC-24-0258.

Reference Type DERIVED
PMID: 39665627 (View on PubMed)

Other Identifiers

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2023-503244-14-00

Identifier Type: CTIS

Identifier Source: secondary_id

2022-002826-29

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

HM-FLTI-101

Identifier Type: -

Identifier Source: org_study_id

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