Pharmacokinetics Variability of Posaconazole (PCZ) and Its Glucuronide Metabolite During Induction and Consolidation Treatments in Patients With Acute Myeloid Leukemia (AML): a Covariate Analysis With the Tablets Formulation and Evaluation of the Potential Risk of Hepatotoxicity

NCT ID: NCT03796533

Last Updated: 2022-02-24

Basic Information

• Recruitment Status: UNKNOWN
• Clinical Phase: NA
• Total Enrollment: 120 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2018-12-10
• Study Completion Date: 2023-04-10

Brief Summary

Among its authorized indications, posaconazole (PCZ) is prescribed for prophylaxis in onco-hematology patients at high risk of invasive fungal infections. This azole antifungal has a low bioavailability. The enteric-coated tablets form available since mid-2015 has significantly improved its pharmacokinetic profile compared to the oral suspension form initially used. According to the recommendations of The European Conference on Infections in Leukemia (ECIL-6), the minimum serum concentration desirable for prophylaxis is 0.7 mg/L. This concentration threshold was difficult to achieve in onco-hematology patients treated with oral suspension.

The investigators retrospectively collected and analyzed 201 results of residual PCZ serum concentrations from 91 onco-hematology patients on Noxafil® tablets prophylaxis. The median concentration of PCZ was 1.08 mg/L. In this study, the pharmacokinetics of tablet-PCZ showed significant inter-individual variability. Thus, while 25% of the concentrations remained below the recommended threshold of 0.7 mg/L (25% percentile = 0.69 mg/L), exposure to PCZ was greater than 2.63 mg/L in 10% of cases. This level of exposure, however, did not have obvious hepatic repercussions. Nevertheless, further studies involving larger cohorts are needed to clarify a hypothetical relationship between serum PCZ concentration and the occurrence of hepatic toxicity.

In addition, the investigators found significant intra-individual variability in PCZ exposure (CV = 48.8%), especially in leukemic patients. This variability is probably related to a modification during the treatment of the physio-pathological conditions of the patient likely to impact the pharmacokinetics of PCZ (absorption, distribution, metabolism, etc.) as well as the effect of possible pharmacokinetic drug interactions.

The metabolism of PCZ is mediated primarily by the uridine diphosphate (UDP)-glucuronosyltransferase 1A4 (UGT1A4) pathway. Although hepatic metabolism of PCZ is low compared with other azoles (such as itraconazole or voriconazole), differences in the metabolic capacity of UGT1A4 may alter PCZ exposure. A previous study of the oral suspension form had shown that low concentrations of PCZ were associated with a high ratio of PCZ-glucuronide / PCZ concentrations. Two genetic variants of the gene encoding UGT1A4 are associated with a decrease in the metabolic clearance of glucuronide drugs via UGT1A4. A recent study suggests less exposure to PCZ in the presence of UGT1A4 polymorphism.

The main objective of the investigator's project is to study prospectively in a homogeneous population of patients treated by intensive chemotherapy for acute myeloid leukemia (induction and consolidation) the pharmacokinetics of PCZ administered in its tablet formulation, and in particular:

• Clinical and biological tolerance of high concentrations of PCZ
• The influence of clinical and demographic covariates on PCZ and PCZ-glucuronide ratio
• The influence of genetic variants of UGT1A4 on PCZ metabolism (PCZ-glucuronide / PCZ ratio).

Conditions

Leukemia, Myeloid, Acute

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: HEALTH_SERVICES_RESEARCH
• Blinding Strategy: NONE

Study Groups

Posaconazole pharmacokinetics

Patients with AML over the age of 18 years treated with intensive chemotherapy in induction and consolidation whose was under antifungal prophylaxis by PCZ formulation tablets.

Group Type: OTHER

Posaconazole

Intervention Type: DRUG

• PCZ will be used as recommended in tablet formulation at an initial dosage of 300 mg twice a day on the first day and then once a day at a dose of 300 mg the following days.
• PCZ prophylaxis will be started on the same day as the start of chemotherapy and will be continued until the end of aplasia.
• The dosage of the PCZ can be adjusted according to the results of the PCZ assay. It may be interrupted if the transaminase level is greater than 3 times higher than normal (3N) or on medical decision.

Interventions

Posaconazole

• PCZ will be used as recommended in tablet formulation at an initial dosage of 300 mg twice a day on the first day and then once a day at a dose of 300 mg the following days.
• PCZ prophylaxis will be started on the same day as the start of chemotherapy and will be continued until the end of aplasia.
• The dosage of the PCZ can be adjusted according to the results of the PCZ assay. It may be interrupted if the transaminase level is greater than 3 times higher than normal (3N) or on medical decision.

Intervention Type: DRUG

Other Intervention Names

PCZ

Eligibility Criteria

Inclusion Criteria

1. Patient aged 18 or over

2. Patients with AML de novo or secondary to myelodysplastic syndrome or therapy-related AML except acute myeloid leukemia (AML3)

3. Patient hospitalized for the treatment of leukemia (induction chemotherapy or consolidation)

4. General state retained (ECOG performance scale ≤ 3)

5. alanine aminotransferase aspartate transaminase (ASAT) and alanine aminotransferase (ALAT) ≤ 2.5 times the upper limit of normal (ULN), total bilirubin ≤ 2 times the ULN, creatinine <150 μmol / L unless these biological abnormalities are related to leukemia

6. Patients affiliated or beneficiaries of a social security scheme (Social Security or Universal Medical Coverage)

7. Having read and understood the information sheet and signed the informed consent

Exclusion Criteria

1. Patients with acute promyelocytic leukemia (AML3)

2. History of uncontrolled cancer for at least two years

3. Patient included in another clinical study that may interfere with the objectives of this study

4. Treatment with antifungal other than posaconazole

5. Severe uncontrolled infection at the time of inclusion

6. Positive serology for HIV 1 or 2 or human T-cell lymphoma virus (HTLV 1) or 2, or active viral infection with hepatitis B virus (HBV) or hepatitis C virus (HCV)

7. Pregnant woman (beta positive HCG) or breastfeeding

8. A woman of childbearing potential who can not justify the use of effective contraception during treatment with Noxafil®

9. Patient incapacitated, under guardianship, curators or safeguard of justice

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Hospices Civils de Lyon

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Locations

Centre Hospitalier Lyon Sud, Hematology department

Pierre-Bénite, , France

Site Status: RECRUITING

Countries

France

Central Contacts

Sophie Ducastelle-lepretre

Role: CONTACT

Sophie.ducastelle-lepretre@chu-lyon.fr

04 78 86 22 36 ext. +33

Mohamed EL HAMRI

Role: CONTACT

mohamed.el-hamri@chu-lyon.fr

04 78 86 22 20 ext. +33

Facility Contacts

Sophie DUCASTELLE-LEPRETRE, MD

Role: primary

Sophie.ducastelle-lepretre@chu-lyon.fr

04 78 86 22 36 ext. +33

Mohamed EL HAMRI, MD

Role: backup

mohamed.el-hamri@chu-lyon.fr

04 78 86 22 20 ext. +33

References

Parant F, Gagnieu MC, Di-Pilla L, Deloire A, Joassard A, Millet A, Barthelemy D, Payen L, Ducastelle-Lepretre S. Impact of UGT1A4 Polymorphisms on the Posaconazole Serum Trough Concentrations in Patients With Acute Myeloid Leukemia Receiving Delayed-Release Tablets. Ther Drug Monit. 2025 Oct 1;47(5):609-618. doi: 10.1097/FTD.0000000000001319. Epub 2025 Mar 4.

Reference Type: DERIVED

PMID: 40044655 (View on PubMed)

Other Identifiers

2018-003488-67

Identifier Type: OTHER

Identifier Source: secondary_id

69HCL18_0429

Identifier Type: -

Identifier Source: org_study_id