The Role of Pyroptosis in Chronic Venous Disease

NCT ID: NCT03744858

Last Updated: 2018-11-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

400 participants

Study Classification

OBSERVATIONAL

Study Start Date

2018-11-12

Study Completion Date

2020-11-12

Brief Summary

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Enrollment criteria and clinical data collection: following the principles of medical ethics, the development of inclusion and exclusion criteria. Selecting 200 cases of chronic venous disease (CVD) according to the Comprehensive Classification System for Chronic Venous Disorders (CEAP) divided into 6 Clinical stages (C1-C6) (Group A). Selecting 200 healthy participants without CVD (C0) as controls (Group B). Blood samples will be collected from both groups. Markers of pyroptosis (NETs, Caspase-1 and Cytokines) will be evaluated between the two groups and between the subgroups, according to clinical stage, in group A.

Detailed Description

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Pyroptosis is a pro-inflammatory form of regulated cell death and is dependent on networks of extracellular fibers (NET,s), on the enzymatic activity of inflammatory proteases that belong to the family of cysteine-dependent aspartate-specific proteases (caspase), and cytokines. The role of inflammation has been previously demonstrated in chronic venous disease (CVD), which is a condition that affects up to 80% of adult population in western countries.

According to the Comprehensive Classification System for Chronic Venous Disorders (CEAP) divided into 6 Clinical stages:

C0: no visible or palpable signs of venous disease. C1: telangiectasies or reticular veins. C2: varicose veins. C3: edema. C4a: pigmentation and eczema. C4b: lipodermatosclerosis and atrophie blanche. C5: healed venous ulcer. C6: active venous ulcer.

The aim of this study is to evaluate the role of pyroptosis in the onset and progression of CVD.

The Investigators will select 200 participants with chronic venous disease (CVD) according to Clinical stage of CEAP (C1-C6 patients) \[Group A\] . The investigators will also select 200 healthy participants without CVD (C0) as controls \[Group B\].

Peripheral vein blood samples will be collected from both groups. Markers of pyroptosis (NETs, Caspase-1 and Cytokines) will be evaluated between the two groups and between the subgroups of group A, according to clinical stage.

Conditions

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Inflammation Veins Complication Varicose Veins Varicose Ulcer

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Group A - Participants with CVD

Patients with chronic venous disease (CVD) will undergo peripheral blood draw. Markers of pyroptosis (NETs, Caspase-1 and Cytokines) will be evaluated in blood samples.

Peripheral blood draw

Intervention Type PROCEDURE

Peripheral vein blood draw will be performed to all participants in order to collect blood samples to be evaluated.

Group B - Healthy Participants

Voluntary healthy subjects will undergo peripheral blood draw. Markers of pyroptosis (NETs, Caspase-1 and Cytokines) will be evaluated in blood samples.

Peripheral blood draw

Intervention Type PROCEDURE

Peripheral vein blood draw will be performed to all participants in order to collect blood samples to be evaluated.

Interventions

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Peripheral blood draw

Peripheral vein blood draw will be performed to all participants in order to collect blood samples to be evaluated.

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Chronic Venous Disease (C1-C6 CEAP classification )

Exclusion Criteria

* Peripheral artery disease
* Malignant Tumors
* Severe cardiovascular and cerebrovascular disease
* Liver and kidney failure
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Catanzaro

OTHER

Sponsor Role lead

Responsible Party

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Prof. Raffaele Serra, MD, Ph.D.

Clinical Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Raffaele Serra, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

University Magna Graecia of Catanzaro

Locations

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CIFL- Interuniversity Center of Phlebolymphology

Catanzaro, , Italy

Site Status

Countries

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Italy

Other Identifiers

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ER.CZ.2018.06

Identifier Type: -

Identifier Source: org_study_id

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