The Sublimated Mare Milk Supplement's Effect on Gut Mucosal Lining After Antibiotics

NCT ID: NCT03657836

Last Updated: 2021-02-08

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

12 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-08-01

Study Completion Date

2020-12-01

Brief Summary

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This clinical trial studies the effect of sublimated mare milk supplement on gut microbiome of patients with acute bronchitis.

Detailed Description

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Food has a significant effect on the health of the body, the relationship between diet and health actualizes the importance of expanding the research of functional products. In mare's milk contains about 40 biological components necessary for the human body: amino acids, fats, enzymes (lysozyme, amylase), microelements (calcium, sodium, potassium, phosphorus, iron, magnesium, copper, iodine, sulfur, cobalt, zinc, bromine) and vitamins (A, C, B1, B2, B6, B12, E, H, PP, beta-carotene, folic acid) in optimally balanced proportions.

A high percentage of nutrients, including vitamins, amino acids, contribute to immunomodulation, increasing the adaptogenic properties of the body. Valiev A demonstrated the effect of the essential fatty acids of mare's milk on immunocompetent cells and non-specific resistance after 6 weeks from the beginning of inclusion in the ration of mare's milk.

Secretory IgA is the main immunoglobulin of mare's milk. The homology of human secretory IgA and mare's was previously demonstrated by cross-reactions using human anti-IgA antiserum.

The mare's milk has a powerful detoxification effect, the mare's microflora neutralizes the effect of mutagens, possesses, replenishes with the necessary complex of nutrients and removes toxins from the body. The milk has a certain degree of antimicrobial effect in relation to opportunistic and pathogenic fungi, bacteria and viruses due to its own microflora.

The mare's milk is rich in active substances, natural enzymes that help regulate intestinal flora, limiting the growth of unwanted bacteria and increasing the growth of bifidobacteria and lactic acid bacteria.

In addition, the composition of mare's milk provided immunoglobulins A, M and G, which act as a marker of those microorganisms that can be pathogenic, which facilitates the protection task.

The high antimicrobial activity of mare's milk is associated with the lysozyme contained in it, immunoglobulins, lactoperoxidase and lactoferrin. Getting into the digestive tract lysozyme has a powerful normalizing effect on the composition of the microbial flora of the mouth and intestines. Lysozyme of breast milk is 100 times more active than lysozyme of cow's milk. It not only inhibits the growth of pathogenic flora, but also promotes the growth of bifidoflora in the intestines of infants.

In the framework of the project, methods for the restoration of the mucos layer of the intestine after antibiotic therapy will be developed. In order to develop the algorithm, preliminary studies will be made of changes in the structure of the intestinal microbiota after antibiotic therapy. Recruiting of patients with infectious / non-infectious pathology is supposed, clinical and laboratory examination, biomaterial sampling during antibiotic therapy, 1-5 days and 1-60 days after completion will be performed. Along with the study of microflora, local immunity studies will be carried out. To study the fundamental foundations, experimental studies will be carried out on laboratory animals. On the basis of the knowledge obtained, an algorithm for the restoration of the mucinous layer of the intestine after antibiotic therapy will be published in the form of methodological recommendations.

Conditions

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Acute Bronchitis

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Dietary supplement and antibiotics

Participants will take a supplement of 1 sachet (20 mg) dissolved in 200 ml of warm water (36-37 °C) once a day for 60 days accompanied with the standard antibiotic therapy (cefuroxime and ceftriaxone) up to 7 days.

Group Type EXPERIMENTAL

Sublimated mare milk supplement

Intervention Type DIETARY_SUPPLEMENT

The dietary supplement consisting of sublimated mare milk packed in single-dose sachet.

Standard antibiotic therapy

Intervention Type DRUG

Patients will be given standard therapy antibiotics cefuroxime and ceftriaxone for up to 7 days period.

Antibiotics only

Participants will take the prescribed antibiotic therapy (cefuroxime and ceftriaxone) up to 7 days.

Group Type OTHER

Standard antibiotic therapy

Intervention Type DRUG

Patients will be given standard therapy antibiotics cefuroxime and ceftriaxone for up to 7 days period.

Interventions

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Sublimated mare milk supplement

The dietary supplement consisting of sublimated mare milk packed in single-dose sachet.

Intervention Type DIETARY_SUPPLEMENT

Standard antibiotic therapy

Patients will be given standard therapy antibiotics cefuroxime and ceftriaxone for up to 7 days period.

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* Patients of both sexes aged 4-5 years
* The voluntary informed consent of the parent to participate in the study
* Established diagnosis of acute upper respiratory tract disease with prescription of antibacterial therapy
* The duration of symptoms of acute upper respiratory tract disease no more than 72 hours
* Lack of prescription of antibacterial drugs in the last 3 months

Exclusion Criteria

* Severe forms of acute infections
* The presence in the history of taking probiotics and antibiotics for three months before admission to hospital
* Presence in the anamnesis of chronic diseases of the digestive tract, and also of any surgeries on the digestive tract
* The presence of severe concomitant diseases of the kidneys, liver, cardiovascular, respiratory and other body systems, cancer, mental and decompensated endocrine diseases, tuberculosis, HIV infection
* Patient involvement in other clinical trials within the last 3 months
* Lack of willingness of the patient to cooperate with the doctor-researcher
Minimum Eligible Age

4 Years

Maximum Eligible Age

5 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Eurasia Invest Ltd.

INDUSTRY

Sponsor Role collaborator

Ministry of Education and Science, Republic of Kazakhstan

OTHER_GOV

Sponsor Role collaborator

Nazarbayev University Medical Center

OTHER

Sponsor Role lead

Responsible Party

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Almagul Kushugulova

Head of the Laboratory of Human Microbiome and Longevity

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Almagul Kushugulova, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

National Laboratory Astana

Locations

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University Medical Center

Astana, , Kazakhstan

Site Status

Countries

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Kazakhstan

References

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Valiev AG. [Features of secondary immune response and status of nonspecific resistance of the rat, fed rations with mare's milk, rich in essential fatty acids]. Vopr Pitan. 2001;70(5):10-3. Russian.

Reference Type BACKGROUND
PMID: 11715690 (View on PubMed)

Pahud JJ, Mach JP. Equine secretory IgA and secretory component. Int Arch Allergy Appl Immunol. 1972;42(2):175-86. doi: 10.1159/000230604. No abstract available.

Reference Type BACKGROUND
PMID: 4622991 (View on PubMed)

Foekel C, Schubert R, Kaatz M, Schmidt I, Bauer A, Hipler UC, Vogelsang H, Rabe K, Jahreis G. Dietetic effects of oral intervention with mare's milk on the Severity Scoring of Atopic Dermatitis, on faecal microbiota and on immunological parameters in patients with atopic dermatitis. Int J Food Sci Nutr. 2009;60 Suppl 7:41-52. doi: 10.1080/09637480802249082. Epub 2009 May 21.

Reference Type BACKGROUND
PMID: 19462320 (View on PubMed)

Wulijideligen, Asahina T, Hara K, Arakawa K, Nakano H, Miyamoto T. Production of bacteriocin by Leuconostoc mesenteroides 406 isolated from Mongolian fermented mare's milk, airag. Anim Sci J. 2012 Oct;83(10):704-11. doi: 10.1111/j.1740-0929.2012.01010.x. Epub 2012 Mar 13.

Reference Type BACKGROUND
PMID: 23035710 (View on PubMed)

Hancock JT, Salisbury V, Ovejero-Boglione MC, Cherry R, Hoare C, Eisenthal R, Harrison R. Antimicrobial properties of milk: dependence on presence of xanthine oxidase and nitrite. Antimicrob Agents Chemother. 2002 Oct;46(10):3308-10. doi: 10.1128/AAC.46.10.3308-3310.2002.

Reference Type BACKGROUND
PMID: 12234868 (View on PubMed)

Palmer AC. Nutritionally mediated programming of the developing immune system. Adv Nutr. 2011 Sep;2(5):377-95. doi: 10.3945/an.111.000570. Epub 2011 Sep 6.

Reference Type BACKGROUND
PMID: 22332080 (View on PubMed)

ADIYAR NURGAZIYEV, YERMEK AITENOV et al. Effect of mare's milk prebiotic supplementation on the gut microbiome and the immune system following antibiotic therapy // B IOD I V E R S I T A S.- Volume 21, Number 11, November 2020 Pages: 5065-5071 DOI: 10.13057/biodiv/d211110

Reference Type RESULT

SAMAT KOZHAKHMETOV, DMITRIY BABENKO. The combination of mare's milk and grape polyphenol extract for treatment of dysbiosis induced by dextran sulfate sodium // B IOD I V E R S I T A S Volume 21, Number 5, May 2020 Pages: 2275-2280 DOI: 10.13057/biodiv/d210558

Reference Type RESULT

Almagul Kushugulova, Samat Kozhakhmetov. Mare's milk as a prospective functional product //Functional Foods in Health and Disease. = 2018; 8(11): 537-543

Reference Type RESULT

Other Identifiers

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AP05135073

Identifier Type: -

Identifier Source: org_study_id

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