Therapeutic Vaccine Plus PD-1 Knockout in Prostate Cancer Treatment

NCT ID: NCT03525652

Last Updated: 2018-05-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE1/PHASE2

Total Enrollment

30 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-02-22

Study Completion Date

2021-08-30

Brief Summary

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This study is to evaluate the safety and efficacy of a therapeutic vaccine in combination with PD-1 knockout T cells in the treatment of advanced prostate cancer.

Detailed Description

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This is a phase 1/2 clinical study investigating the safety and efficacy of a therapeutic vaccine in combination with PD-1 knockout T cells in the treatment of advanced prostate cancer. The therapeutic vaccine is a customized product involving ex vivo treatment of the patient's peripheral blood mononuclear cells with a recombinant fusion protein (PAP-GM-CSF) to activate the expression of the antigen that would activate the immune function to kill cancer cells. The PD-1 knockout engineered T cells are also prepared using patient's T cells in which PD-1 gene will be knocked out using CRISPR Cas9 technology. The therapeutic vaccine and PD-1 knockout T cells will be infused back to the patient in 3 times with a 2-week interval.

Conditions

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Prostate Cancer

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Participants

Study Groups

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Therapeutic vaccine

Therapeutic vaccine will be prepared ex vivo using the peripheral mononuclear cells from the patients and the vaccine (as maturated dendritic cells) will be infused back to the patients in 3 times with a 2-week interval.

Group Type ACTIVE_COMPARATOR

Therapeutic vaccine

Intervention Type BIOLOGICAL

The therapeutic vaccine will be custom prepared ex vivo using the peripheral mononuclear cells from the patient and the vaccine which presented as maturated dendritic cells will be infused back to the patients in 3 times.

Therapeutic vaccine plus PD-1 knockout

Therapeutic vaccine and PD-1 knockout T cells will be prepared ex vivo using the white cells from the patients and the vaccine (as maturated dendritic cells) and maturated PD-1 knockout T cells will be infused back to the patients in 3 times.

Group Type EXPERIMENTAL

Therapeutic vaccine

Intervention Type BIOLOGICAL

The therapeutic vaccine will be custom prepared ex vivo using the peripheral mononuclear cells from the patient and the vaccine which presented as maturated dendritic cells will be infused back to the patients in 3 times.

PD-1 Knockout T Cells

Intervention Type BIOLOGICAL

PD-1 knockout T cells will be custom prepared ex vivo using the white blood cells from the patient and the maturated PD-1 knockout T cells will be infused back to the patients in 3 times.

PD-1 knockout T cells

PD-1 knockout T cells will be prepared ex vivo using the white cells from the patients and the maturated PD-1 knockout T cells will be infused back to the patients in 3 times.

Group Type ACTIVE_COMPARATOR

PD-1 Knockout T Cells

Intervention Type BIOLOGICAL

PD-1 knockout T cells will be custom prepared ex vivo using the white blood cells from the patient and the maturated PD-1 knockout T cells will be infused back to the patients in 3 times.

Interventions

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Therapeutic vaccine

The therapeutic vaccine will be custom prepared ex vivo using the peripheral mononuclear cells from the patient and the vaccine which presented as maturated dendritic cells will be infused back to the patients in 3 times.

Intervention Type BIOLOGICAL

PD-1 Knockout T Cells

PD-1 knockout T cells will be custom prepared ex vivo using the white blood cells from the patient and the maturated PD-1 knockout T cells will be infused back to the patients in 3 times.

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* • Histologically confirmed prostate cancer (stage IV, according to NCCN Clinical Practice Guidelines in Oncology: Prostate Cancer, Version 2.2017)

* Evidence of metastasis in the soft tissue and/or bone.
* Progressive androgen independent castrate resistant prostate cancer.
* Serum PSA ≥ 5.0 ng/mL
* Estimated life expectancy ≥ 6 months.
* Castrate level of testosterone (\< 50 ng/dL) achieved via medical or surgical castration.
* Adequate hematologic, renal and liver function.

Exclusion Criteria

* • Presence of known lung, liver, or brain metastases, malignant pleural effusions, or malignant ascites.

* Presence of moderate to severe pain treating with opioid analgesics within 21 days prior to registration.
* ECOG score ≥ 2.
* Any other systemic therapy for prostate cancer (except for medical castration).
* Participation in previous study using Provenge (Sipuleucel-T) or similar product.
* Known pathologic long-bone fractures, imminent pathologic long-bone fracture (cortical erosion on radiography \> 50%) or spinal cord compression.
* Known malignancies other than prostate cancer requiring active treatment within 6 months.
* A requirement for systemic immunosuppressive therapy for any reason.
* A history of allergic reactions attributed to compounds of similar chemical or biologic composition to this product or granulocyte-macrophage colony-stimulating factor.
* Any infection requiring parenteral antibiotic therapy or causing fever (temp \> 100.5°F or \> 38.1°C) within 1 week prior to registration.
* Any medical intervention or other condition which, in the opinion of the Principal Investigator could compromise adherence with study requirements or otherwise compromise the study's objectives.
* Treatment with any of the following medications or interventions within 28 days of registration:

Systemic use of corticosteroids, External beam radiation therapy or surgery, Use of non-steroidal antiandrogens Dietary and herbal supplements, as well as alternative treatments that have evidence of hormonal and/or anticancer properties (e.g., prostate cancer (PC) -SPES or PC-SPEC) and saw palmetto, Megestrol acetate (Megace®), diethylstilbesterol (DES), or cyproterone acetate, ++Ketoconazole, 5-alpha-reductase inhibitors, Treatment with any other investigational product Treatment with chemotherapy High dose calcitriol \[1,25(OH)2Vitamin D\] (i.e., \> 0.5 mcg/day). Initiation or discontinuation of bisphosphonate therapy
Minimum Eligible Age

18 Years

Maximum Eligible Age

70 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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Guangzhou Anjie Biomedical Technology Co., Ltd.

INDUSTRY

Sponsor Role collaborator

University of Technology, Sydney

OTHER

Sponsor Role collaborator

The First Affiliated Hospital of Guangdong Pharmaceutical University

OTHER

Sponsor Role lead

Responsible Party

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Size Chen

Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Size Chen, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

The First Affiliated Hospital of Guangdong Pharmaceutical University

Locations

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First Affiliated Hospital of Guangdong Pharmaceutical University

Guangzhou, Guangdong, China

Site Status RECRUITING

Professor Size Chen

Guangzhou, Guangdong, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Size Chen, MD, PhD

Role: CONTACT

+8613710956393

Zhizhou Huang, MSc

Role: CONTACT

+8613268258980

Facility Contacts

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Guobiao Huang

Role: primary

86-20-39352064

Size Chen, MD,PhD

Role: primary

+8613720956393

Zhizhou Huang, MSc

Role: backup

+8613268258980

Other Identifiers

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2018-007

Identifier Type: -

Identifier Source: org_study_id

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