Investigation of Dietary Nitrate Effects in Hypertension-induced Target Organ Damage

NCT ID: NCT03088514

Last Updated: 2024-02-20

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE2
• Total Enrollment: 149 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2017-03-23
• Study Completion Date: 2023-11-15

Brief Summary

This study aims to determine whether dietary inorganic nitrate (in beetroot juice) is able to reduce overall thickening of the heart (left ventricular hypertrophy or LVH) and stiffness of the arteries when given to patients with persistently raised blood pressure (hypertension). Half the patients will receive the beetroot juice containing inorganic nitrate and half will receive beetroot juice from which the inorganic nitrate has been removed. The volunteers will take the juice every day for 4 months.

Detailed Description

In hypertension persistent raised blood pressure (BP) is associated with endothelial dysfunction, arterial stiffness and left ventricular (LV) remodeling that are key phenomena associated with the pathogenesis and complications of hypertension.

One of the main substances that the healthy endothelium produces that is responsible for maintaining the patency of blood vessels is nitric oxide (NO). In hypertension, one of the key pathogenic effects is the dysfunction of the endothelium characterized by a decrease in ability to generate nitric oxide (NO). Previous studies have shown that dietary inorganic nitrate supplementation lowers blood pressure (Kapil et al. 2015), however, whether this approach might also improve endothelial function and LV remodeling is unknown. The effects of inorganic nitrate are due to its conversion in the body to inorganic nitrite and thereafter to NO.

This study will assess the effects of dietary inorganic nitrate on LVH using cardiac magnetic resonance imaging (NITRATE-LVH arm). In addition, the effects of dietary inorganic nitrate on central aortic blood pressure, arterial stiffness using pulse wave velocity and endothelial function using flow mediated dilatation will be evaluated (NITRATE-CBP arm).

Conditions

Hypertension

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: QUADRUPLE

Study Groups

NITRATE-LVH intervention

70ml of beetroot juice (approximately 6-8 mmol of inorganic nitrate) once a day for 4 months

Group Type: EXPERIMENTAL

Beetroot juice

Intervention Type: DIETARY_SUPPLEMENT

Beetroot juice (70ml daily) with or without inorganic nitrate

NITRATE-LVH placebo

70ml of beetroot juice (no inorganic nitrate) once a day for 4 months

Group Type: PLACEBO_COMPARATOR

Beetroot juice

Intervention Type: DIETARY_SUPPLEMENT

Beetroot juice (70ml daily) with or without inorganic nitrate

NITRATE-CBP intervention

70ml of beetroot juice (approximately 6-8 mmol of inorganic nitrate) once a day for 4 month

Group Type: EXPERIMENTAL

Beetroot juice

Intervention Type: DIETARY_SUPPLEMENT

Beetroot juice (70ml daily) with or without inorganic nitrate

NITRATE-CBP placebo

70ml of beetroot juice (no inorganic nitrate) once a day for 4 months

Group Type: PLACEBO_COMPARATOR

Beetroot juice

Intervention Type: DIETARY_SUPPLEMENT

Beetroot juice (70ml daily) with or without inorganic nitrate

Interventions

Beetroot juice

Beetroot juice (70ml daily) with or without inorganic nitrate

Intervention Type: DIETARY_SUPPLEMENT

Eligibility Criteria

Inclusion Criteria

1. Patients will be enrolled following an informed consent. The subject will be able to understand and comply with protocol requirements, instructions and protocol restrictions.

2. Aged 18-80 years.

3. The study subjects will be hypertensives with evidence of difficulty treating to target BP (daytime ABPM 135-170/85-105 mmHg) on 1 or more antihypertensive agents, with insufficient efficacy or intolerance of medications.

4. For NITRATE LVH, echocardiographic evidence of LV hypertrophy (LV mass indexed to body surface area (BSA); males >115g/m2; females >95 g/m2).

5. Patients will have been established on an antihypertensive treatment regime for at least 1 month by the time of participation in the study and will not require changes in pharmacological intervention for the duration of the trial.

Exclusion Criteria

Unless specified, a subject will not be eligible for inclusion in this study if any of the following criteria apply:

1. History of chronic viral hepatitis (including presence of hepatitis B surface antigen or hepatitis C antibody), or other chronic hepatic disorders.

2. History of increased liver function tests (ALT, AST) due to acute or chronic liver conditions, 3x above the upper limit of normal or bilirubin 1.5x above the upper limit of normal at screening.

3. Renal impairment with creatinine clearance (eGFR) of <50 ml/min at screening.

4. Patients with diabetes mellitus, defined by previous history of diabetes or HbA1c >6.5% (>48 mmol/mol) at screening.

5. Subjects with LDLc, >7.5 mmol/l. TG level >10mmol/l.

6. History of heart failure defined as NYHA class II - IV or those with known LV dysfunction (EF<40%) regardless of symptomatic status

7. History of malignancy within the past 5 years, other than non-melanoma skin cancer.

8. Current life-threatening condition other than vascular disease (e.g. very severe chronic airways disease, HIV positive, life-threatening arrhythmias) that may prevent a subject from completing the study.

9. Use of an investigational device or investigational drug within 30 days or 5 half-lives (whichever is longer) preceding the first dose of study medication.

10. Subjects who will commence or who are likely to commence regular treatment with non-steroidal anti-inflammatory drugs (NSAIDs) (other than aspirin), from screening until study completion.

11. Any non-stable dosing of ongoing medication regimens throughout the study trial.

12. Drug abuse within the past 6 months.

13. The subject has a three-month prior history of regular alcohol consumption exceeding an average weekly intake of > 28 units (or an average daily intake of greater than 3 units) for males, or an average weekly intake of > 21 units (or an average daily intake of greater than 2 units) for females. 1 unit is equivalent to a half-pint (284mL) of beer/lager; 25mL measure of spirits or 125mL of wine.

14. Any other subject whom the Investigator deems unsuitable for the study (e.g. due to other medical reasons, laboratory abnormalities, expected study medication noncompliance, or subject's unwillingness to comply with all study-related study procedures).

15. Subjects with rheumatoid arthritis, connective tissue disorders and other conditions known to be associated with chronic inflammation (e.g. Inflammatory Bowel Disease).

16. Subjects with any acute infection, or recent systemic (oral or IV) antibiotics within 1 month of screening, or significant trauma (burns, fractures).

17. Subjects who have donated more than 500 mL of blood within 56 days prior to the study medication administration.

18. Self reported use of anti-microbial mouthwash or tongue scrapes.

19. Concomitant xanthine oxidase inhibitors (such as allopurinol).

20. Known history of significant claustrophobia, previous intolerance of CMR imaging or known (or suspected) incompatible metallic implant.

21. Pregnancy.

22. Allergy to gadolinium-based contrast agents used for CMR.

23. Patients with known LVH caused by another established pathology diagnosed prior to or at screening e.g. severe aortic stenosis, hypertrophic cardiomyopathy, amyloidosis and Fabry's disease.

• For criteria 18, patients can enter the trial if they discontinue the use of anti-microbial mouthwash for the duration of the clinical trial.
• nCriteria 20 and 22 do not apply to participants who will not have a CMR scan in the NITRATE-CBP arm

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 80 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Barts & The London NHS Trust

OTHER

Sponsor Role: collaborator

Queen Mary University of London

OTHER

Sponsor Role: lead

Responsible Party

Amrita Ahluwalia

Professor of Vascular Pharmacology

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Amrita Ahulwalia, BSc PhD

Role: PRINCIPAL_INVESTIGATOR

Queen Mary University of London

Locations

Queen Mary University of London

London, , United Kingdom

Countries

United Kingdom

References

Kapil V, Khambata RS, Robertson A, Caulfield MJ, Ahluwalia A. Dietary nitrate provides sustained blood pressure lowering in hypertensive patients: a randomized, phase 2, double-blind, placebo-controlled study. Hypertension. 2015 Feb;65(2):320-7. doi: 10.1161/HYPERTENSIONAHA.114.04675. Epub 2014 Nov 24.

Reference Type: BACKGROUND

PMID: 25421976 (View on PubMed)

Lau CWZ, Hamers AJP, Rathod KS, Shabbir A, Cooper J, Primus CP, Davies C, Mathur A, Moon JC, Kapil V, Ahluwalia A. Randomised, double-blind, placebo-controlled clinical trial investigating the effects of inorganic nitrate in hypertension-induced target organ damage: protocol of the NITRATE-TOD study in the UK. BMJ Open. 2020 Jan 21;10(1):e034399. doi: 10.1136/bmjopen-2019-034399.

Reference Type: DERIVED

PMID: 31969369 (View on PubMed)

Other Identifiers

10/H0703/98

Identifier Type: -

Identifier Source: org_study_id