Antioxidant Use in Diabetes to Reduce Oxidative Stress

NCT ID: NCT03056014

Last Updated: 2022-03-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

TERMINATED

Clinical Phase

EARLY_PHASE1

Total Enrollment

8 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-11-01

Study Completion Date

2020-03-12

Brief Summary

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Dietary supplementation with antioxidant vitamins, such as Vitamin C and Vitamin E, reduces malformation rates in embryos of diabetic animals. However, human trials exploring the benefits of these antioxidant vitamins have produced unsatisfactory results in trials designed to alleviating diabetic retinopathy, cardiovascular disease, and preeclampsia in pregnancies. The investigators hypothesize that more potent, and better-targeted antioxidants, such as N-acetylcysteine (NAC) and Polyunsaturated Fatty Acids(PUFA), will be successful in preventing birth defects in the offspring of women with diabetes.

Detailed Description

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Specific Aim 1. Recruit non pregnant women with T1DM and investigate the efficacy of dietary NAC on ameliorating oxidative stress Study design. Diabetic patients will be provided with NAC or placebo for 14 days, while receiving usual clinical care. The oxidative stress status will be assessed by measuring biomarkers in blood samples pre and post intervention. In addition to a placebo control group, three treatment groups including Group 1 (NAC 600 mg/day), Group 2 (NAC 1200 mg/day), and Group 3 (NAC 1800 mg/day) will be studied. The choice of dosage of NAC is based on published studies, which show effectiveness of NAC in 600 or 1200 mg day in alleviating oxidative stress in diabetic patients, both in men and women, without adverse side effects. The investigators will use the supplement company TwinLab for our study. The university of Maryland Pharmacy department will analyze the NAC for purity prior to starting the study. At day 7, participants will be called via phone assess for symptoms and side effects from medications. All participants will be called. At the end of 14 days, the patients will return to the CDE with a survey asking about compliance with medication and any side effects. They will also bring the pill bottle so that study personnel can do a pill count. At this time blood will be draw for the biomarker levels to look for changes in oxidative stress.

Specific Aim 2. To investigate effect of PUFAs on ameliorating oxidative stress in diabetic non-pregnant women.

Study design: The investigators will recruit a new group of Non-pregnant women with T1DM will be enrolled and randomly assigned to placebo or one of three treatment groups. Study volunteers will be divided into 1 of 3 groups. PUFA; Group 1 (1000 mg/day) or Group 2 (2000 mg/day) or Group 3(placebo). The treatment regimens, sample collection, biomarker assessment, side effect monitoring and statistical analysis will be performed as described in SA 1. The investigators will perform an analysis of the oxidative stress biomarkers as described in SA1. The investigators will use TwinLab as our commercial supplier of PUFA for our trial. There fish oil supplements have been involved in greater than 40 published trials. The fish oil supplement will be analyzed by the University of Maryland pharmacy department prior to starting the study to analyze for purity.

Specific Aim 3: To investigate the potential secondary benefit of NAC/PUFA on kidney function and lipid profile. Urine and serum samples will be collected on all enrolled subjects at day 0 and Day 14 to monitor for improvement in microalbumin in the urine and lipid profile in the serum. Previous studies have shown improvements in LDL with supplementation of NAC. The investigators will look at how various dosages effect the improvement in microalbumin and lipid profile.

Conditions

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Ameliorating Oxidative Stress in Type 1 Diabetes

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Participants
Double blind

Study Groups

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N-acetylcysteine 600 mg

Group Type ACTIVE_COMPARATOR

N-acetyl cysteine

Intervention Type DRUG

giving varying doses of NAC in order to determine which reduces oxidative stress.

N-acetylcysteine 1200 mg

Group Type ACTIVE_COMPARATOR

N-acetyl cysteine

Intervention Type DRUG

giving varying doses of NAC in order to determine which reduces oxidative stress.

placebo

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type DIETARY_SUPPLEMENT

L-alanine placebo pill to determine if effect is supplement related or random effect.

PUFA 1000 mg

Group Type ACTIVE_COMPARATOR

omega 6 Fish oil ( PUFA)

Intervention Type DIETARY_SUPPLEMENT

giving varying doses of PUFA in order to determine which reduces oxidative stress.

PUFA 2000 mg

Group Type ACTIVE_COMPARATOR

omega 6 Fish oil ( PUFA)

Intervention Type DIETARY_SUPPLEMENT

giving varying doses of PUFA in order to determine which reduces oxidative stress.

Placebo

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type DIETARY_SUPPLEMENT

L-alanine placebo pill to determine if effect is supplement related or random effect.

Interventions

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N-acetyl cysteine

giving varying doses of NAC in order to determine which reduces oxidative stress.

Intervention Type DRUG

omega 6 Fish oil ( PUFA)

giving varying doses of PUFA in order to determine which reduces oxidative stress.

Intervention Type DIETARY_SUPPLEMENT

Placebo

L-alanine placebo pill to determine if effect is supplement related or random effect.

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* hemoglobin a1c \<10
* type 1 diabetes

Exclusion Criteria

* pregnancy
* BMI \> 40
* greater than 1 alcoholic beverages per week
* any tobacco use
* prescribed nitroglycerin, HIV protease inhibits, corticosteroids, cephalosporins, or blood thinners
* vascular complications(history of coronary artery disease, cerebral vascular accident, transient ischemic attack, claudication).
Minimum Eligible Age

18 Years

Maximum Eligible Age

44 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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University of Maryland, Baltimore

OTHER

Sponsor Role lead

Responsible Party

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Sarah Crimmins

Assistant Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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University of Maryland, Baltimore

Baltimore, Maryland, United States

Site Status

Countries

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United States

Other Identifiers

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HP-00067782

Identifier Type: -

Identifier Source: org_study_id

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