Durvalumab and Endocrine Therapy in ER+/Her2- Breast Cancer After CD8+ Infiltration Effective Immune-Attractant Exposure

NCT ID: NCT02997995

Last Updated: 2020-09-30

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE2
• Total Enrollment: 61 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2017-02-15
• Study Completion Date: 2020-08-28

Brief Summary

This is an open-label, multicentric, international, phase II trial testing aromatase inhibitors in combination with durvalumab in patients with CD8+ T cell infiltration (>10% CD8+ T cells in the tumor). The trial includes two sequences: The first part of the treatment will consist in 4-6 weeks treatment with immune-attractants; in the second part, CD8+ patients will receive 6 months of durvalumab combined with exemestane.

Detailed Description

The study is conducted in 2 parts:

Part 1: lymphocyte attraction. After the screening phase, the patient will receive immune-attractant combined with exemestane for six weeks.

As immune-attractants are added over the course of the study, they will appear as subsequent appendices in the full protocol.

Up to 4 cohorts may be tested sequentially in this design until up to 240 evaluable patients have been treated.

The first cohort of patients will receive tremelimumab (3 mg/kg, single infusion) combined with exemestane (25 mg daily). In each cohort, an interim analysis will be performed after 30 patients in order to potentially stop the cohort (if less than 25% of patients present >10% CD8+ cells in the tumor after 3 weeks). If all 4 cohorts are closed and the target number of 56 patients for part 2 has not been reached, additional patients will be recruited and treated with the best performing immune-attractant treatment based on the part I results. From the moment 56 patients are included in part 2, no more patients will be entered in part 1.

After three weeks (+/- 3 days), a tumor biopsy will be done. Patients who present >10% CD8+ cells in the tumor after 3 weeks and remain eligible will be included in the second part of the trial (patients who do not present CD8+ T cells on the 3-week biopsy will be treated at the investigator's choice).

Part 2: lymphocyte activation (anti-PD1 treatment) Four to six weeks after immune-attractant start, patients having >10% CD8+ cells in the tumor will receive durvalumab 1500 mg Q4W (equivalent to 20 mg/kg Q4W) IV, combined with exemestane (25 mg daily), for six months.

Part 2 will include two steps. In the first step, we will include 23 patients. If 2 or more pathological complete responses are observed in these 23 patients, the part 2 will move to step 2. 33 additional patients will be included in the step 2.

Conditions

Breast Cancer; Estrogen Receptor Positive Tumor; Menopause; Hormone Antagonist

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Immune-attractant/lymphocyte activation

After the screening phase, the patient will receive immune-attractant combined with exemestane for six weeks. After three weeks (+/- 3 days), a tumor biopsy will be done. Patients who present \>10% CD8+ cells in the tumor after 3 weeks and remain eligible will be included in the second part of the trial i.e. lymphocyte activation. In this second part, patients will receive durvalumab 1500 mg Q4W (equivalent to 20 mg/kg Q4W) IV, combined with exemestane (25 mg daily), for six months. The pathological response will be checked by surgery.

Group Type: EXPERIMENTAL

Immune-attractant

Intervention Type: DRUG

The first cohort patients will receive tremelimumab (3 mg/kg, single infusion) as immune-attractants combined with exemestane (25 mg daily).

Durvalumab

Intervention Type: DRUG

Durvalumab (lymphocyte activation) will be administrated at a dose of 1500 mg Q4W (equivalent to 20 mg/kg Q4W) IV, combined with exemestane (25 mg daily), for six months

Biopsy

Intervention Type: PROCEDURE

After three weeks (+/- 3 days) of immune-attractants, a tumor biopsy will be done. Patients who present \>10% CD8+ cells in the tumor after 3 weeks will receive the Durvalumab

Interventions

Immune-attractant

The first cohort patients will receive tremelimumab (3 mg/kg, single infusion) as immune-attractants combined with exemestane (25 mg daily).

Intervention Type: DRUG

Durvalumab

Durvalumab (lymphocyte activation) will be administrated at a dose of 1500 mg Q4W (equivalent to 20 mg/kg Q4W) IV, combined with exemestane (25 mg daily), for six months

Intervention Type: DRUG

Biopsy

After three weeks (+/- 3 days) of immune-attractants, a tumor biopsy will be done. Patients who present \>10% CD8+ cells in the tumor after 3 weeks will receive the Durvalumab

Intervention Type: PROCEDURE

Other Intervention Names

Tremelimumab; MEDI4736

Eligibility Criteria

Inclusion Criteria

1. Age ≥18 years post-menopausal according to one of the following criteria:

• Age >60 years
• Or Bilateral ovariectomy
• Or Age ≤60, with an uterus and presenting an amenorrhea of more than 12 months and FSH and estradiol in the postmenopausal range
• Or Age ≤60, without an uterus and FSH and estradiol in the postmenopausal range

2. Histologically proven invasive breast cancer eligible to neoadjuvant endocrine therapy according to multidisciplinary tumor board.

Note: Multicentric/multifocal tumors are allowed if all share the same characteristics

3. cT2-T4, any N; cT2 are eligible only if the clinical tumor size is >3 cm

4. Non metastatic, M0 (according to clinical staging)

5. Luminal A patients ER-positive by immunohistochemistry (IHC) according to the following criteria (local assessment): Grade I or II AND ER-positive (≥60%) AND Ki67 <20%

6. Her2-negative by IHC (score 0 or 1+) and/or fluorescent in situ hybridization (FISH)/chromogenic in situ hybridization (CISH) negative according to local assessment

7. CD8+ T Cell infiltration defined as >10% cells stained with anti-CD8 monoclonal antibody by IHC at the 3-week biopsy (applicable for inclusion in part 2 only)

8. Available tumor samples from baseline biopsy

9. World Health Organization (WHO)/Eastern Cooperative Oncology Group (ECOG) performance status of 0 or 1 at enrolment

10. Adequate organ and marrow function as defined below:

• Hemoglobin ≥9.0 g/dL
• Absolute neutrophil count ≥1.5 × 10⁹/L
• Platelet count ≥100 × 10⁹/L
• Serum bilirubin ≤1.5 × upper limit of normal (ULN). This will not apply to patients with confirmed Gilbert's syndrome, who will be allowed in consultation with their physician
• Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) ≤2.5 × ULN
• Adequate renal function as determined by CKD-EPI formula (using actual body weight)

11. Willingness and ability to comply with scheduled visits, treatment plan, laboratory tests, and other trial procedures

12. Written informed consent obtained prior to performing any protocol-related procedures, including screening evaluations

Exclusion Criteria

1. Inflammatory breast cancer

2. No prior exposure to immune-mediated therapy including, but not limited to, other anti-CTLA-4, anti-PD-1, anti-PD-L1, and anti-programmed cell death ligand 2 (anti-PD-L2) antibodies, excluding therapeutic anticancer vaccines

3. Any concurrent chemotherapy, investigational product (IP), biologic therapy for cancer treatment

4. Previous Radiotherapy treatment to more than 30% of the bone marrow;

5. Major surgical procedure (as defined by the Investigator) within 28 days prior to the first dose

6. History of allogenic organ transplantation

7. Active or prior documented autoimmune or inflammatory disorders (including inflammatory bowel disease [eg, colitis or Crohn's disease], diverticulitis with the exception of diverticulosis, celiac disease or other serious gastrointestinal chronic conditions associated with diarrhea), systemic lupus erythematosus, Sarcoidosis syndrome, or Wegener syndrome (granulomatosis with polyangiitis), Graves' disease, rheumatoid arthritis, hypophysitis, uveitis, etc within the past 3 years prior to the start of treatment. The following are exceptions to this criterion:

• Patients with vitiligo or alopecia
• Patients with hypothyroidism (eg, following Hashimoto syndrome) stable on hormone replacement or psoriasis not requiring systemic treatment

8. Any condition that, in the opinion of the Investigator, would interfere with the evaluation of investigational product or interpretation of patient safety or study results, including ongoing or active infection, symptomatic congestive heart failure, uncontrolled hypertension, unstable angina pectoris, cardiac arrhythmia, interstitial lung disease, or psychiatric illness/social situations that would limit compliance with study requirement, substantially increase risk of incurring adverse events from investigational products, or compromise the ability of the patient to give written informed consent

9. Mean QT interval corrected for heart rate using Fridericia's formula (QTcF) ≥470 ms

10. History of active primary immunodeficiency

11. Known history of active tuberculosis

12. Active infection including hepatitis B, hepatitis C, or human immunodeficiency virus (HIV)

13. Current or prior use of immunosuppressive medication within 14 days before the first dose. The following are exceptions to this criterion:

• Intranasal, inhaled, topical steroids, or local steroid injections (eg, intra-articular injection)
• Systemic corticosteroids at physiologic doses not exceeding 10 mg/day of prednisone or its equivalent
• Steroids as premedication for hypersensitivity reactions (eg, CT scan premedication)

14. Receipt of live, attenuated vaccine within 30 days prior to the first dose of IP.

Note: Patients, if enrolled, should not receive live vaccine during the study and up to 30 days after the last dose of IP

15. Known allergy or hypersensitivity to any medicinal product used in the trial or any excipient

• Minimum Eligible Age: 18 Years
• Eligible Sex: FEMALE
• Accepts Healthy Volunteers: No

Sponsors

Breast International Group

OTHER

Sponsor Role: collaborator

UNICANCER

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Fabrice Andre, Prof

Role: PRINCIPAL_INVESTIGATOR

Gustave Roussy, Cancer Campus, Grand Paris

Locations

Centre Hospitalier cote Basque

Bayonne, , France

Institut Bergonié

Bordeaux, , France

Centre François Baclesse

Caen, , France

Centre Hospitalier de Cahors

Cahors, , France

Centre Hôspitalier de Cholet

Cholet, , France

Centre George François Leclerc

Dijon, , France

Institut Daniel Hollard Groupe Hôspitalier

Grenoble, , France

Centre Oscar Lambret

Lille, , France

CHU Limoges

Limoges, , France

Centre Hospitalier Bretagne Sud

Lorient, , France

Centre Léon Bérard

Lyon, , France

Institut Paoli Calmettes

Marseille, , France

Institut Curie Site Paris

Paris, , France

Hôpital Saint Louis APHP

Paris, , France

Centre Hospitalier Perpignan

Perpignan, , France

Institut Jean Godinot

Reims, , France

Institut Curie Hôpital René Huguenin

Saint-Cloud, , France

Centre Paul Strauss

Strasbourg, , France

Institut Claudius Regaud

Toulouse, , France

CHU Bretonneau - Centre Henry Kaplan

Tours, , France

Gustave Roussy

Villejuif, , France

ICO Badalona

Badalona, , Spain

Hospital Clinic Barcelona

Barcelona, , Spain

HU Vall Hebron

Barcelona, , Spain

HU Arnau de Vilanova

Lleida, , Spain

CIO Clara Campal

Madrid, , Spain

HU Ramon y Cajal

Madrid, , Spain

Hospital Clínico Universitario de Valencia

Valencia, , Spain

Sahlgrenska University Hospital

Gothenburg, , Sweden

Countries

France; Spain; Sweden

Other Identifiers

UCBG-105

Identifier Type: OTHER

Identifier Source: secondary_id

BIG 16-01

Identifier Type: OTHER

Identifier Source: secondary_id

2016-000764-42

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

UC-0140/1606

Identifier Type: -

Identifier Source: org_study_id