Glycan Attachment Specificity, Toward ROtavirus Vaccine IMprovement GASTROVIMc (Clinical Investigation)

NCT ID: NCT02902445

Last Updated: 2022-04-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

611 participants

Study Classification

OBSERVATIONAL

Study Start Date

2017-02-28

Study Completion Date

2020-10-15

Brief Summary

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The GASTROVIM research explores the links between individual genetic susceptibility, genetic variability of rotavirus strains and effectiveness of immunization with the rotavirus vaccination: a clinical investigation to assess glycan attachment specificity, toward rotavirus vaccine improvement.

Detailed Description

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The work will be carried out in France and in a tropical area of population with diverse geographical origins: the Guyana populated with Native Americans, people of European descent, people of Asian (Hmong) and a large source population African.

The first aim of the project will be to characterize the specificity of the VP8 \* HBGA of RotaTeq and Rotarix vaccines in order to ensure their binding characteristics glycans are similar to those of recent P8 clinical strains circulating in France and were maintained in the culture passages.

The second objective will be to validate the impact of recently described polymorphisms HBGAs on susceptibility to infection by RVA through GASTROVIMc prospective clinical research.

The third objective will be to determine the relative role of recognition and HBGAs ganglioside in the initial attachment and viral entry.

The expression of HBGAs and ganglioside by permissive cell lines in culture human stem RVA be manipulated to define the role of glycans in attachment and infection.

Conditions

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Gastroenteritis Acute Gastroenteritis

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Case group

250 children from Nantes University Hospital and 150 children from Guyana Hospital admitted in paediatric emergency unit for diagnosed rotavirus acute gastroenteritis: rotavirus rapid screen test and oral swab for polymorphism exploration

rotavirus rapid screen test

Intervention Type BIOLOGICAL

According to routine care

polymorphism exploration

Intervention Type GENETIC

from an oral swab collected after parents consent

Control group

250 children from Nantes University Hospital and 150 children from Guyana Hospital admitted in paediatric emergency unit without signs of infection and / or digestive clinical picture evocative of gastroenteritis.

Paired with a case upon ethnicity or origin if impossible to match the ethnicity of the case.

oral swab for polymorphism exploration

polymorphism exploration

Intervention Type GENETIC

from an oral swab collected after parents consent

Interventions

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rotavirus rapid screen test

According to routine care

Intervention Type BIOLOGICAL

polymorphism exploration

from an oral swab collected after parents consent

Intervention Type GENETIC

Eligibility Criteria

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Inclusion Criteria

* 0 to 16.
* giving consent to participate if applicable (\>6yo)
* Parents informed and written consent obtained
* admitted in paediatric emergency for wether an acute gastroenteritis (case group) or anything else than an infection or gastroenteritis (control group)

Exclusion Criteria

* case group: rotavirus rapid screen test negative; viral gastroenteritis other than rotavirus; bacterial gastroenteritis
* control group: Family and / or patient unable to full informed consent to research; Infectious Pathology context in general.
Maximum Eligible Age

16 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Nantes University Hospital

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Christèle GRAS-LEGUEN, MD PhD

Role: PRINCIPAL_INVESTIGATOR

Nantes University Hospital

Locations

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Nantes University Hospital

Nantes, Loire-Atlantique, France

Site Status

Countries

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France

Other Identifiers

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RC15_0329

Identifier Type: -

Identifier Source: org_study_id

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