Influence of Vaginal Lactoferrin on Amniotic Fluid Cytokines to Avoid Pregnancy Complications

NCT ID: NCT02843984

Last Updated: 2016-07-26

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

60 participants

Study Classification

INTERVENTIONAL

Study Start Date

2011-03-31

Study Completion Date

2012-03-31

Brief Summary

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Objective: To evaluate the influence of vaginal Lactoferrin administration on amniotic fluid concentration of 47 cytokines, chemokines and growth factors.

Detailed Description

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60 women undergoing genetic amniocentesis will be enrolled at the Obstetric Unit, University of Ferrara. The selected patients will be randomly assigned to receive 300 mg of vaginal Lactoferrin to obtain 3 groups: A, 20 untreated patients; B and C (20 patients each one) respectively treated 4 and 12 h before amniocentesis. Cytokines, chemokines and growth factors concentrations will be quantified by a magnetic bead Luminex multiplex immunoassays panel technology.

Amniotic fluid samples will be obtained by transabdominal amniocentesis and the sample not required for clinical purposes will be centrifuged to remove particulate material and the supernatants will be aliquoted and stored at -80°C until assay.

Conditions

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Pregnancy

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

NONE

Study Groups

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A - untreated

The patients will be not treated with vaginal lactoferrin

Group Type NO_INTERVENTION

No interventions assigned to this group

B - 4 hrs treatment

The patients will be administered with a single dose of 300 mg vaginal lactoferrin 4 hours prior to mid-trimester genetic amniocentesis.

Group Type ACTIVE_COMPARATOR

Lactoferrin

Intervention Type DRUG

C - 12 hrs treatment

The patients will be administered with a single dose of 300 mg vaginal lactoferrin 12 hours prior to mid-trimester genetic amniocentesis.

Group Type ACTIVE_COMPARATOR

Lactoferrin

Intervention Type DRUG

Interventions

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Lactoferrin

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* singleton physiological pregnancy;
* maternal age as the only indication to foetal karyotyping

Exclusion Criteria

* assumption of drugs interfering with the immune system;
* previous miscarriages;
* pregnancy at risk for maternal or foetal disease;
* lactose intolerance.
Minimum Eligible Age

25 Years

Maximum Eligible Age

45 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Università degli Studi di Ferrara

OTHER

Sponsor Role lead

Responsible Party

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Alessandro Trentini

PhD

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Obstetric Unit, University of Ferrara

Cona, Ferrara, Italy

Site Status

Countries

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Italy

Other Identifiers

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PRUa1GR-2013-00000220-B

Identifier Type: -

Identifier Source: org_study_id

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