Serum Sphingolipidomic Analyses in Healthy, Diabetic and Prediabetic Subjects

NCT ID: NCT02826759

Last Updated: 2016-07-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

200 participants

Study Classification

OBSERVATIONAL

Study Start Date

2016-07-31

Study Completion Date

2016-11-30

Brief Summary

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This study is designed to compare the serum sphingolipidomic analyses in healthy, pre-diabetic and diabetic subjects. age, sex and BMI are matched among these three groups. As ceramide, sphingosine, sphingosine-1-phosphate and sphinganine are involved in inflammation, immunity and cancer, investigators proposed a hypothesis that sphingosine-1-phosphate and other sphingolipids may be associated with the progress of type 2 diabetes. sphingolipids may be a biomarker for diabetes.

Detailed Description

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The first purpose of this study is to determine whether serum sphingolipid metabolites associate significantly with the weight among type 2 diabetes. 60 patients with diagnosed type 2 diabetes will be recruited. The serum concentrations of sphingolipid metabolites of 20 type 2 diabetic patients with obesity will be compared to age- and sex-matched series of 40 type 2 patients without obesity.

The second purpose of this study is to determine whether serum sphingolipid metabolites associate significantly with the process and severity of type 2 diabetes. The serum concentrations of sphingolipid metabolites of 20 subjects with type 2 diabetes will be compared to age-, sex- and BMI-matched series of healthy and pre-diabetic subjects. Investigators speculate that the serum concentration of sphingolipid metabolites are positively related with the progression of diabetes. In order to discover why serum sphingolipid metabolites correlates with the progression of diabetes, detailed information on HbA1c, duration of diabetes history and insulin resistance defined by homeostatic model assessment (HOMA-IR) will be analysed. These three parameters may affect the serum concentrations of sphingolipid metabolites.

Conditions

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Diabetes Mellitus, Type 2 Prediabetic State

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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serum sphingolipid metabolites in healthy subjects

Healthy subjects are classified according to BMI into three subgroups: healthy normal weight subjects, healthy overweight subjects and healthy subjects with obesity. Age and sex are matched among three subgroups. serum sphingolipid metabolites including sphingosine-1-phosphate will be compared.

No interventions assigned to this group

serum sphingolipid metabolites in diabetic subjects

Diagnosed diabetic patients are divided into three subgroups: diabetic patients with normal weight, overweight and obesity. age and sex are matched.

diabetes

Intervention Type OTHER

the exposure of interests are obesity, diabetes and insulin-resistance

serum sphingolipid metabolites in the progression of diabetes

serum sphingolipid metabolites are compared among three age-, sex- and body mass index-matched subgroups: healthy subjects, subjects with pre-diabetes, subjects with diabetes

diabetes

Intervention Type OTHER

the exposure of interests are obesity, diabetes and insulin-resistance

serum sphingolipid metabolites and HbA1c

diabetic subjects are divided by HbA1c level. the cut-offs are 7% and 9%. serum sphingolipid metabolites will be tested among diabetic patients with HbA1c \<7%, 7%-9% and \>9%

diabetes

Intervention Type OTHER

the exposure of interests are obesity, diabetes and insulin-resistance

serum sphingolipid metabolites in insulin-resistant subjects

comparison of serum sphingolipid metabolites in newly diagnosed insulin-resistant subjects and age-, sex- and BMI-matched healthy controls.

diabetes

Intervention Type OTHER

the exposure of interests are obesity, diabetes and insulin-resistance

Interventions

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diabetes

the exposure of interests are obesity, diabetes and insulin-resistance

Intervention Type OTHER

Other Intervention Names

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insulin-resistance

Eligibility Criteria

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Inclusion Criteria

* clinical diagnosis of diabetes mellitus, type 2
* clinical diagnosis of prediabetic status
* older than 18 and younger than 90 years-old
* clinical diagnosis of insulin-resistance
* clinical diagnosis of newly onset of diabetes mellitus, type 2
* those who are willing to participate in the trial and sign the consent form

Exclusion Criteria

* any cardiovascular disease (myocardial infarction, heart failure, cerebrovascular accident)
* missing information of BMI
* sever liver, kidney dysfunction
* sever pancreatitis or those who received pancreatectomy
* on medications of hormone, immunosuppressive therapy or drugs that may affect the bioactivity or concentration of sphingolipids (e.g. asprin )
* patients on pregnancy
* sever systematic diseases including carcinoma, mental disorder, sever anemia et al.
Minimum Eligible Age

18 Years

Maximum Eligible Age

90 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Chinese Medical Association

NETWORK

Sponsor Role collaborator

First Affiliated Hospital Xi'an Jiaotong University

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jing Sui

Role: STUDY_DIRECTOR

First Affiliated Hospital Xi'an Jiaotong University

Locations

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The First Affiliated Hospital of Xi'an Jiaotong University

Xi'an, Shaanxi, China

Site Status

Countries

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China

Central Contacts

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Jing Sui

Role: CONTACT

0086-18991989230

Facility Contacts

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Bingyin Shi

Role: primary

0086-13700298366

jing Sui

Role: backup

0086-18991989230

References

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Sui J, He M, Wang Y, Zhao X, He Y, Shi B. Sphingolipid metabolism in type 2 diabetes and associated cardiovascular complications. Exp Ther Med. 2019 Nov;18(5):3603-3614. doi: 10.3892/etm.2019.7981. Epub 2019 Sep 6.

Reference Type DERIVED
PMID: 31602237 (View on PubMed)

Other Identifiers

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13040470432

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

2013YK20

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

KYLLSL201312701

Identifier Type: -

Identifier Source: org_study_id

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