A Study of the Safety and Efficacy of EBV Specific T-cell Lines

NCT ID: NCT02580539

Last Updated: 2025-05-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

12 participants

Study Classification

INTERVENTIONAL

Study Start Date

2015-11-30

Study Completion Date

2025-05-31

Brief Summary

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This study evaluates the safety and efficacy of EBV-specific T-cell lines to treat patients suffering from high EBV viral titers not responding to standard of care therapies and to treat EBV-related lymphoma. The study will recruit 6 patients to receive autologous T cells or a T cell line derived from the patient's allogeneic donor (in the case of stem cell transplant recipients), and 6 patients to receive a T-cell line prepared from a matched or partially matched related donor.

Detailed Description

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Epstein-Barr virus (EBV) is a member of the herpes virus family and infects up to 95% of individuals over their lifetime. Most initial infections occur in childhood and after a brief flu-like illness, the virus enters a phase of latency.

Patients who receive a bone marrow transplant or an organ transplant take medications drugs that weaken their immune systems. In these contexts, the virus can "reactivate" and cause very serious problems, such as lymphoma. For unknown reasons, people with a normal immune system can also develop lymphoma due to EBV.

The purpose of this study is to test the safety and efficacy of immune cells (T lymphocytes) that are specifically "taught" to recognize the virus-infected cells and to eliminate them. This "education" occurs is done over during a 2 weeks period (approximately), in the research laboratory. The cells are then transfused into the patient.

Conditions

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Epstein-Barr Virus Infections Post-Transplant Lymphoproliferative Disorder Lymphoma

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Autologous or allogenic (stem cell donor) T cells

Subjects receive an autologous anti-EBV T-cell line or a T-cell line derived from the patient's allogeneic (stem cell transplant) donor.

Group Type EXPERIMENTAL

Group A

Intervention Type BIOLOGICAL

Peptide-stimulated T cells 2 x 10\^7/m\^2

Allogeneic "third party" T cells

Subjects receive a T-cell line from a matched or partially matched related donor.

Group Type EXPERIMENTAL

Group B

Intervention Type BIOLOGICAL

Peptide-stimulated T cells per dose-escalation protocol

Interventions

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Group A

Peptide-stimulated T cells 2 x 10\^7/m\^2

Intervention Type BIOLOGICAL

Group B

Peptide-stimulated T cells per dose-escalation protocol

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* Capacity to provide informed consent
* Age ≥ 18 years old
* Confirmed treatment-refractory EBV reactivation or EBV-related lymphoma
* ECOG of 2 or less

Exclusion Criteria

* Medical condition requiring a corticosteroid dose greater than Prednisone 0.5mg/kg/day (or equivalent) at the time of the infusion.
* Patient has received T-cell depleting antibodies or stem cell transplantation in the 28 days prior to proposed date of anti-EBV T-cell line infusion
* Patient has received a solid organ transplant in the 3 months prior to proposed date of anti-EBV T-cell line infusion.
* Pregnant or nursing females
* Life expectancy of less than 3 months due to a condition unrelated to the EBV- related disease.
* Active uncontrolled GVHD
* Active uncontrolled SOT rejection episode

DONOR ELIGIBILITY: An allogeneic donor must be a first-degree relative with at least 3/6 HLA compatibility, have consented to donate peripheral blood mononuclear cells, and fulfill the same criteria for stem cell donation according to the hospital's standard operating procedure.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Dr. Jean-Sebastien Delisle, MD, PhD

OTHER

Sponsor Role lead

Responsible Party

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Dr. Jean-Sebastien Delisle, MD, PhD

Clinician-Scientist, Hematopoietic Cell Transplantation Program

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Jean-Sebastien Delisle, MD,PhD

Role: PRINCIPAL_INVESTIGATOR

Maisonneuve-Rosemont Hospital

Locations

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Hôpital Maisonneuve-Rosemont

Montreal, Quebec, Canada

Site Status

Countries

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Canada

Related Links

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Other Identifiers

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CER15020

Identifier Type: -

Identifier Source: org_study_id

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