Extended Clomiphene Citrate Regimen in Women With Polycystic Ovary Syndrome

NCT ID: NCT02381184

Last Updated: 2016-08-23

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE2/PHASE3
• Total Enrollment: 160 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2014-06-30
• Study Completion Date: 2016-07-31

Brief Summary

One hundred and thirty six anovulatory women with CC-resistant PCOS were scheduled randomly into two equal groups. Group A (n=68); received CC (100 mg/day from cycle day 3 for 10 days) for up to six cycles. Group B (n=68) underwent LOD and followed up for 6 months. The primary outcome was the ovulation rate in each group; secondary outcomes were midcycle endometrial thickness and serum estradiol, midluteal serum progesterone, and the rates of clinical pregnancy and abortion.

Detailed Description

This prospective randomized trial included 136 anovulatory women with CC-resistant PCOS among those attending the Gynecology Outpatient Clinics of, Benha University Hospital, and private practice settings, Alkalubia, Egypt from August 2012 to August 2014. The study protocol was approved by the Local Ethics Committee and written informed consents were obtained before the study commenced. Diagnosis of PCOS was based on the Rotterdam criteria (18), whereby diagnosis of PCOS require the presence of two of three criteria after the exclusion of other etiologies, i.e., oligomenorrhea and/or anovulation, clinical and/or biochemical signs of hyperandrogenism, and/or polycystic ovaries on ultrasound. Inclusion criteria: 1- Age: between 18-35 years. 2- Period of infertility > 2 years. 3- Serum level of FSH <10 U/L in the early follicular phase. 4- All women were CC-resistant PCOS, as they failed to ovulate with a dose of CC of 150 mg/day for 5 days per cycle for at least three consecutive cycles. 5- All women had patent Fallopian tubes proved by hysterosalpingography or laparoscopy and their partners satisfied the normal parameters of semen analysis according to the modified WHO criteria (19). Exclusion criteria: 1- Infertility due to causes other than CC- resistant PCOS or due to combined factors. 2- Body mass index (BMI) ≥35 Kg/m². 3- The use of metformin, gonadotropins, hormonal contraception or diet regimen within the last 6 months. 4- Women with congenital adrenal hyperplasia, hyperprolactinaemia or abnormal thyroid function. 5- Hypersensitivity or contraindications to Letrozole or clomiphene treatment. 6- Previous LOD. The study was started two months after last CC treatment cycle to eliminate any post-treatment effect of CC.

At admission, a thorough evaluation of all participants was carried out via history taking and physical examination, which included anthropometric measurements. A baseline transvaginal ultrasound examination was done using 7-10 MHZ probe (Voluson 730 PRO V, GE Healthcare, USA) to measure endometrial thickness and exclude any pelvic pathology before treatment. Basal hormonal assays were performed on day 3 of the spontaneous or progestin induced cycle and included measurement of the serum concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone.

Randomization was done according to a computer generated random numerical table into two equal groups. Treatment allocation was concealed by using sequentially numbered opaque sealed envelopes. Envelopes were opened consecutively by a third person (study nurse), who assigned patients to the extended Clomiphene Citrate group (Group A) or LOD group (Group B). Once allocated, the treatment was revealed to both the investigator and the patient. Group A (n =68) assigned to receive100 mg of clomiphene citrate (Clomid®; Hoechst Marion Russel, Cairo, Egypt) daily starting on day 3 of spontaneous or progestin induced cycle for 10 days. Group B (n =68) underwent LOD. Laparoscopy was performed under general anesthesia, using three-puncture technique. Each ovary was cauterized perpendicular to its antimesenteric border at four points, each for 4 seconds at 40 W with a mixed current, using a monopolar electrosurgical needle (Karl Storz, ND, Germany). Each puncture was about 4 mm in diameter and 6-8 mm in depth. The ovary was cooled by irrigation with normal saline solution. Any, intraoperative or postoperative complication was reported. The follow-up was started from the next cycle after ovarian drilling up to 6 months.

Starting from cycle day 9, ultrasound scans were repeated daily to monitor follicle growth (number and mean diameter). Ultrasound examination was done by the same doctor to avoid interobserver variability and he was blinded to the treatment groups. When the optimal follicle size was reached (i.e. mean follicular diameter ≥ 18 mm), HCG 10,000 IU (Epifasi, EPICO, Egypt) was given intramuscular to trigger ovulation. No HCG was given and protected sexual intercourse was advised, if there were ≥ 3 follicles with a diameter ≥16mm to avoid the risk of ovarian hyperstimulation syndrome and multiple gestations. Intrauterine insemination was performed 36 - 40 hours after HCG injection using soft tip catheter.

Serum E2 and the endometrial thickness were measured on the day of HCG administration. The endometrial thickness was measured in a sagittal plane in the fundus of the uterus (point of maximal thickness) from the echogenic interface at the junction of the endometrium and myometrium Ovulation was confirmed when midluteal (day 21of the cycle) serum progesterone was ≥ 5 ng/mL. Serum β-hCG was determined 2 weeks after HCG injection for diagnosis of biochemical pregnancy. Sonographic evidence of an intrauterine gestational sac at 6 weeks gestation was considered an evident of a clinical pregnancy.

Treatment continued for up to six months. Patients were counseled for further lines of treatment if there was no ovulation as confirmed by midluteal serum progesterone level less than 5 ng /ml within 3 months.

The primary outcome was the ovulation rate in each group. Secondary outcomes were the endometrial thickness and serum estradiol (pg/ml), on the day of the hCG injection, midluteal serum progesterone (ng/ml) and the rates of clinical pregnancy and first trimester spontaneous abortion. The ovulation rate was calculated as the percentage of ovulatory cycles per total observed cycles. The cumulative pregnancy rate was defined as the number of pregnant patients to the total number of patients. The abortion rate was defined as the percentage of miscarriage during the first 12 weeks of gestation per total pregnancies, while the biochemical miscarriage was considered if pregnancy loss occurred in any confirmed biochemical pregnancy without ultrasound evidence of intrauterine pregnancy.

Conditions

Infertility

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Clomiphene Citrate group (Group A)

Group A (n =68) assigned to receive100 mg of clomiphene citrate (Clomid®; Hoechst Marion Russel, Cairo, Egypt) daily starting on day 3 of spontaneous or progestin induced cycle for 10 days.

Group Type: ACTIVE_COMPARATOR

clomiphene citrate

Intervention Type: DRUG

receive100 mg of clomiphene citrate (Clomid®; Hoechst Marion Russel, Cairo, Egypt) daily starting on day 3 of spontaneous or progestin induced cycle for 10 days.

laparoscopic ovarian drilling (LOD) Group B

Group B (n =68) underwent LOD. Laparoscopy was performed under general anesthesia, using three-puncture technique. Each ovary was cauterized perpendicular to its antimesenteric border at four points, each for 4 seconds at 40 W with a mixed current, using a monopolar electrosurgical needle (Karl Storz, ND, Germany). Each puncture was about 4 mm in diameter and 6-8 mm in depth. The ovary was cooled by irrigation with normal saline solution. Any, intraoperative or postoperative complication was reported. The follow-up was started from the next cycle after ovarian drilling up to 6 months

Group Type: ACTIVE_COMPARATOR

laparoscopic ovarian drilling (LOD)

Intervention Type: PROCEDURE

Laparoscopy was performed under general anesthesia, using three-puncture technique

Interventions

clomiphene citrate

receive100 mg of clomiphene citrate (Clomid®; Hoechst Marion Russel, Cairo, Egypt) daily starting on day 3 of spontaneous or progestin induced cycle for 10 days.

Intervention Type: DRUG

laparoscopic ovarian drilling (LOD)

Laparoscopy was performed under general anesthesia, using three-puncture technique

Intervention Type: PROCEDURE

Other Intervention Names

Clomid

Eligibility Criteria

Inclusion Criteria

• Age: between 18-35 years
• Period of infertility > 2 years
• Serum level of FSH <10 U/L in the early follicular phase
• All women were CC-resistant PCOS, as they failed to ovulate with a dose of CC of 150 mg/day for 5 days per cycle for at least three consecutive cycles
• All women had patent Fallopian tubes proved by hysterosalpingography or laparoscopy and their partners satisfied the normal parameters of semen analysis according to the modified WHO criteria

Exclusion Criteria

• Infertility due to causes other than CC- resistant PCOS or due to combined factors
• Body mass index (BMI) ≥35 Kg/m²
• The use of metformin, gonadotropins, hormonal contraception or diet regimen within the last 6 months
• Women with congenital adrenal hyperplasia, hyperprolactinaemia or abnormal thyroid function
• Hypersensitivity or contraindications to Letrozole or clomiphene treatment
• Previous LOD

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 35 Years
• Eligible Sex: FEMALE
• Accepts Healthy Volunteers: No

Sponsors

Benha University

OTHER

Sponsor Role: lead

Responsible Party

khalid abd aziz mohamed

principal investigator

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

ahmed morad

Role: PRINCIPAL_INVESTIGATOR

assistant professor

khalid mohamed

Role: STUDY_CHAIR

lecturer

Locations

Benha Faculty of Medicine

Banhā, El Qaluobia, Egypt

Countries

Egypt

Other Identifiers

khalid-walid

Identifier Type: -

Identifier Source: org_study_id