Cytokine Profile in Children With Celiac Disease

NCT ID: NCT02244047

Last Updated: 2014-09-18

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

66 participants

Study Classification

INTERVENTIONAL

Study Start Date

2013-10-31

Study Completion Date

2014-06-30

Brief Summary

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Celiac disease (CD) is an immune-mediated systemic disease that is elicited by consumption of gluten and related prolamines in genetically susceptible individuals. Not only genetic but also environmental factors play an important role in CD pathogenesis. CD patients have imbalance in the gut microbiota, they have reduced number of Bididobacterium species in feces and biopsies.

Up till now, only effective treatment for CD is life long adherence to gluten free diet. If gluten free diet is not strict that leads over the years to complications of disease, such as autoimmune diseases, psychiatric diseases, osteoporosis etc. That may be caused by continuous recirculation of activated immune cells between the inflamed organ and the periphery. To avoid complications of disease in long term the investigators want to test specific probiotic bacteria from Bifidobacteria genus, that has has been in vitro studies recognized as anti-inflammatory.

Hypothesis

1. Children with celiac disease on gluten free diet have a higher level of pro-inflammatory cytokine (TNF-alpha) and anti-inflammatory cytokine (IL-10) in comparison with healthy controls.
2. 3 months after daily probiotic consumption TNF-alpha level decrease and IL-10 level increase.

In the investigators research will be selected 70 children, age from 2 to 18 years, divided in different groups:

1. Group: 25 children with celiac disease on GFD for at least 3 months and will receive probiotic for 3 months.
2. Group: 25 children with celiac disease on GFD for at least 3 months and will receive placebo for 3 months.
3. Group: 20 healthy children

Detailed Description

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Conditions

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Celiac Disease

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

SINGLE

Participants

Study Groups

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Bifidobacterium breve

Bifidobacterium breve BR03 and B632 powder containing 10/9 CFU daily dosage in a period of 3 months

Group Type ACTIVE_COMPARATOR

Bifidobacterium breve

Intervention Type DRUG

Placebo

Placebo in the same powder packages as Bifidobacterium breve

Group Type ACTIVE_COMPARATOR

Placebo (for Bifidobacterium breve)

Intervention Type DRUG

Interventions

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Bifidobacterium breve

Intervention Type DRUG

Placebo (for Bifidobacterium breve)

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* celiac disease on gluten free diet

Exclusion Criteria

* acute or chronic diseases,
* permanent use of medication and
* ingestion of antibiotics at least one month prior to study
Minimum Eligible Age

1 Year

Maximum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Slovenian Research Agency

OTHER

Sponsor Role collaborator

University Medical Centre Maribor

OTHER

Sponsor Role lead

Responsible Party

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Martina Klemenak

medical doctor, resident at Pediatric department

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Martina Klemenak, md

Role: PRINCIPAL_INVESTIGATOR

University Medical Centre Maribor

References

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Klemenak M, Dolinsek J, Langerholc T, Di Gioia D, Micetic-Turk D. Administration of Bifidobacterium breve Decreases the Production of TNF-alpha in Children with Celiac Disease. Dig Dis Sci. 2015 Nov;60(11):3386-92. doi: 10.1007/s10620-015-3769-7. Epub 2015 Jul 2.

Reference Type DERIVED
PMID: 26134988 (View on PubMed)

Other Identifiers

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CEL-BIF

Identifier Type: -

Identifier Source: org_study_id

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