Establishment of NAFLD Cohort and Development of Fibrosis Markers

NCT ID: NCT02206841

Last Updated: 2025-05-15

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

1000 participants

Study Classification

OBSERVATIONAL

Study Start Date

2014-01-01

Study Completion Date

2030-12-31

Brief Summary

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This study is designed for establishment of non-alcoholic fatty liver disease patients cohort to development of markers to predict histologic progression of liver fibrosis.

Detailed Description

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* Acoustic radiation force impulse elastography and transient elastography
* Liver tissue (frozen tissue, paraffin block)
* Whole blood, Serum
* Fat amount ratio CT (Visceral adipose tissue, Subcutaneous adipose tissue amount)
* Body composition analyzer (InBody scale):Total fat/muscle mass and appendicular skeletal muscle mass
* Pulmonary function test with post-bronchodilator response and DLCo
* EKG, EchoCG, Heart CT (Coronary calcium score), and Pusle wave velocity (AI index, arterial stiffness)
* Brain MRI or CT
* Upper esophagogastroscopy and colonoscopy
* Berlin score questionnaire and Polysomnography

Conditions

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Fibrosis of Liver

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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NAFLD

Patients with suspected nonalcoholic fatty liver disease will be screened. Of all patients fulfilling inclusion criteria, baseline characteristics will be obtained. In addition, laboratory, radiologic evaluations such as ARFI, SWE, and transient elastography will be performed. A diagnostic liver biopsy will be performed for analysis of steatosis and fibrosis. Parts of the remaining liver tissue will be stored by frozen tissue and paraffin block for future study. Several serum and plasma samples are collected of patients and stored for future analysis such as targeted SNP arrays, super-enhancer RNA (eRNA) expression, whole exome sequencing, RNA chip sequencing, RNA microarray, genomic DNA, metabolomics, fecal microbiome, metabolite, metagenome/metatranscriptome analyses.

Liver biopsy

Intervention Type PROCEDURE

Percutaneously liver biopsy will be performed for evaluate steatosis and fibrosis.

ARFI

Intervention Type DEVICE

Acoustic radiation force impulse (ARFI) imaging will be performed for evaluate fibrosis of liver.

SWE

Intervention Type DEVICE

Supersonic shear wave elastography (SWE) will be performed for evaluate fibrosis of liver.

Transient elastography

Intervention Type DEVICE

Transient elastography will be performed for evaluate fibrosis of liver.

Interventions

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Liver biopsy

Percutaneously liver biopsy will be performed for evaluate steatosis and fibrosis.

Intervention Type PROCEDURE

ARFI

Acoustic radiation force impulse (ARFI) imaging will be performed for evaluate fibrosis of liver.

Intervention Type DEVICE

SWE

Supersonic shear wave elastography (SWE) will be performed for evaluate fibrosis of liver.

Intervention Type DEVICE

Transient elastography

Transient elastography will be performed for evaluate fibrosis of liver.

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

* Patients with histologically confirmed fatty liver disease
* Patients with radiologically confirmed fatty liver disease

Exclusion Criteria

* History of significant alcohol consumption
* Viral hepatitis
* Autoimmune hepatitis
* Metabolic diseases (e.g. hemochromatosis, M. Wilson, alpha 1-antitrypsin deficiency)
* Hepatotoxic medication (e.g. amiodarone)
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Seoul National University Boramae Hospital

OTHER

Sponsor Role lead

Responsible Party

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Won Kim

M.D, PhD

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Won Kim, Professor

Role: PRINCIPAL_INVESTIGATOR

SMG-SNU Boramae Medical Center

Locations

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Seoul Metropolitan Government Seoul National University

Seoul, , South Korea

Site Status RECRUITING

Countries

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South Korea

Central Contacts

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Won Kim, MD,PhD

Role: CONTACT

8228702233

Saekyung Joo, MD

Role: CONTACT

821089619285

Facility Contacts

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Won Kim, MD, PhD

Role: primary

8228702233

References

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Koo BK, Joo SK, Kim D, Bae JM, Park JH, Kim JH, Kim W. Additive effects of PNPLA3 and TM6SF2 on the histological severity of non-alcoholic fatty liver disease. J Gastroenterol Hepatol. 2018 Jun;33(6):1277-1285. doi: 10.1111/jgh.14056. Epub 2018 Feb 26.

Reference Type RESULT
PMID: 29193269 (View on PubMed)

Koo BK, Um SH, Seo DS, Joo SK, Bae JM, Park JH, Chang MS, Kim JH, Lee J, Jeong WI, Kim W. Growth differentiation factor 15 predicts advanced fibrosis in biopsy-proven non-alcoholic fatty liver disease. Liver Int. 2018 Apr;38(4):695-705. doi: 10.1111/liv.13587. Epub 2017 Sep 30.

Reference Type RESULT
PMID: 28898507 (View on PubMed)

Joo SK, Kim W, Kim D, Kim JH, Oh S, Lee KL, Chang MS, Jung YJ, So YH, Lee MS, Bae JM, Kim BG. Steatosis severity affects the diagnostic performances of noninvasive fibrosis tests in nonalcoholic fatty liver disease. Liver Int. 2018 Feb;38(2):331-341. doi: 10.1111/liv.13549. Epub 2017 Sep 5.

Reference Type RESULT
PMID: 28796410 (View on PubMed)

Kim D, Kim W, Joo SK, Bae JM, Kim JH, Ahmed A. Subclinical Hypothyroidism and Low-Normal Thyroid Function Are Associated With Nonalcoholic Steatohepatitis and Fibrosis. Clin Gastroenterol Hepatol. 2018 Jan;16(1):123-131.e1. doi: 10.1016/j.cgh.2017.08.014. Epub 2017 Aug 18.

Reference Type RESULT
PMID: 28823829 (View on PubMed)

Kim JY, Park KJ, Hwang JY, Kim GH, Lee D, Lee YJ, Song EH, Yoo MG, Kim BJ, Suh YH, Roh GS, Gao B, Kim W, Kim WH. Activating transcription factor 3 is a target molecule linking hepatic steatosis to impaired glucose homeostasis. J Hepatol. 2017 Aug;67(2):349-359. doi: 10.1016/j.jhep.2017.03.023. Epub 2017 Mar 30.

Reference Type RESULT
PMID: 28365312 (View on PubMed)

Koo BK, Kim D, Joo SK, Kim JH, Chang MS, Kim BG, Lee KL, Kim W. Sarcopenia is an independent risk factor for non-alcoholic steatohepatitis and significant fibrosis. J Hepatol. 2017 Jan;66(1):123-131. doi: 10.1016/j.jhep.2016.08.019. Epub 2016 Sep 4.

Reference Type RESULT
PMID: 27599824 (View on PubMed)

Lee MS, Bae JM, Joo SK, Woo H, Lee DH, Jung YJ, Kim BG, Lee KL, Kim W. Prospective comparison among transient elastography, supersonic shear imaging, and ARFI imaging for predicting fibrosis in nonalcoholic fatty liver disease. PLoS One. 2017 Nov 27;12(11):e0188321. doi: 10.1371/journal.pone.0188321. eCollection 2017.

Reference Type RESULT
PMID: 29176844 (View on PubMed)

Lee DH, Sung SU, Lee YK, Lim IH, Jang H, Joo SK, Park JH, Chang MS, So YH, Kim W; Innovative Target Exploration of NAFLD (ITEN) Consortium. A sequential approach using the age-adjusted fibrosis-4 index and vibration-controlled transient elastography to detect advanced fibrosis in Korean patients with non-alcoholic fatty liver disease. Aliment Pharmacol Ther. 2022 Apr;55(8):994-1007. doi: 10.1111/apt.16766. Epub 2022 Jan 9.

Reference Type DERIVED
PMID: 35005800 (View on PubMed)

Kim D, Kim W, Joo SK, Han J, Kim JH, Harrison SA, Younossi ZM, Ahmed A. Association between body size-metabolic phenotype and nonalcoholic steatohepatitis and significant fibrosis. J Gastroenterol. 2020 Mar;55(3):330-341. doi: 10.1007/s00535-019-01628-z. Epub 2019 Sep 18.

Reference Type DERIVED
PMID: 31535207 (View on PubMed)

Kim D, Kim W, Joo SK, Kim JH, Harrison SA, Younossi ZM, Ahmed A. Predictors of nonalcoholic steatohepatitis and significant fibrosis in non-obese nonalcoholic fatty liver disease. Liver Int. 2019 Feb;39(2):332-341. doi: 10.1111/liv.13983. Epub 2018 Oct 27.

Reference Type DERIVED
PMID: 30298568 (View on PubMed)

Other Identifiers

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NAFLD_cohort

Identifier Type: -

Identifier Source: org_study_id

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